The datafile contains data associated with analytical chemistry, fish gene expression, in vitro bioassays, chemical prioritization, and Attagene data for surface water monitoring and caged fish studies on the South Platte River (Colorado, USA) in 2018 and 2019. This dataset is associated with the following publication: Cavallin, J., J. Beihoffer, B. Blackwell, A. Cole, D. Ekman, R. Hofer, A. Jastrow, J. Kinsey, K. Keteles, E. Maloney, J. Parman, D. Winkelman, and D. Villeneuve. Effects-based monitoring of bioactive compounds associated with municipal wastewater treatment plant effluent discharge to the South Platte River, Colorado, USA. ENVIRONMENTAL POLLUTION. Elsevier Science Ltd, New York, NY, USA, 289: 117928, (2021).

The present study highlights the utility of bioeffects-based monitoring in conjunction with analytical chemical measurements of surface waters on the Colorado River associated with a historically bioactive wastewater treatment plant effluent. Concurrent with chemical monitoring and in vitro bioactivity measurements, in situ caged fish systems were employed to evaluate the potential bioavailability of predicted biologically-active contaminants associated with ER, GR, and PPAR-associated activities. The present study compares the effects of a wastewater treatment plant facility upgrade on bioactive contaminant loading. This dataset is associated with the following publication: Cavallin, J., W. Battaglin, J. Beihoffer, B. Blackwell, P. Bradley, A. Cole, D. Ekman, R. Hofer, J. Kinsey, K. Keteles, D. Winkelman, and D. Villeneuve. Effects-Based Monitoring of Bioactive Chemicals Discharged to the Colorado River Before and After a Municipal Wastewater Treatment Plant Replacement. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 55(2): 974-984, (2021).

The present study highlights the utility of bioeffects-based monitoring in conjunction with analytical chemical measurements of surface waters on the Colorado River associated with a historically bioactive wastewater treatment plant effluent. Concurrent with chemical monitoring and in vitro bioactivity measurements, in situ caged fish systems were employed to evaluate the potential bioavailability of predicted biologically-active contaminants associated with ER, GR, and PPAR-associated activities. The present study compares the effects of a wastewater treatment plant facility upgrade on bioactive contaminant loading. This dataset is associated with the following publication: Cavallin, J., W. Battaglin, J. Beihoffer, B. Blackwell, P. Bradley, A. Cole, D. Ekman, R. Hofer, J. Kinsey, K. Keteles, D. Winkelman, and D. Villeneuve. Effects-Based Monitoring of Bioactive Chemicals Discharged to the Colorado River Before and After a Municipal Wastewater Treatment Plant Replacement. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 55(2): 974-984, (2021).

Water-borne contaminants were monitored in 69 tributaries of the Laurentian Great Lakes in 2010 and 2014 using semipermeable membrane devices (SPMDs), and polar organic chemical integrative samplers (POCIS). Analyses included 185 chemicals (143 detected) including PAHs, legacy and current-use pesticides, fire retardants, pharmaceuticals, fragrances, and others. Hazard quotients were calculated by dividing detected concentrations by biological effect concentrations reported in the ECOTOX Knowledgebase (Toxicity quotients, TQs) or ToxCast database (Exposure Activity Ratios, EARs). This dataset is associated with the following publication: Alvarez, D., S. Corsi, L. De Cicco, D. Villeneuve, and A. Baldwin. Identifying chemicals and mixtures of potential biological concern detected in passive samplers from Great Lakes tributaries using high-throughput data and biological pathways. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA,

Water-borne contaminants were monitored in 69 tributaries of the Laurentian Great Lakes in 2010 and 2014 using semipermeable membrane devices (SPMDs), and polar organic chemical integrative samplers (POCIS). Analyses included 185 chemicals (143 detected) including PAHs, legacy and current-use pesticides, fire retardants, pharmaceuticals, fragrances, and others. Hazard quotients were calculated by dividing detected concentrations by biological effect concentrations reported in the ECOTOX Knowledgebase (Toxicity quotients, TQs) or ToxCast database (Exposure Activity Ratios, EARs). This dataset is associated with the following publication: Alvarez, D., S. Corsi, L. De Cicco, D. Villeneuve, and A. Baldwin. Identifying chemicals and mixtures of potential biological concern detected in passive samplers from Great Lakes tributaries using high-throughput data and biological pathways. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA,

The occurrence of chemicals of emerging concern (CECs) and potential effects to aquatic biota in select tributaries of the St. Croix River in Minnesota and Wisconsin were measured. In 2011, treated wastewater effluent samples were collected from 22 sites in the St. Croix River Basin to determine total estrogenic activity. In 2012, wastewater effluent was collected at five select locations based on total estrogenicity and analyzed for CECs. In addition, surface water, bottom sediment, resident fish, and resident crayfish were collected upstream and downstream from effluent discharges. Water samples were analyzed for CECs (wastewater indicator compounds, steroid hormones, sterols, and pharmaceuticals). Bottom sediment samples were analyzed for a subset of CECs (wastewater indicator compounds, steroid hormones, and sterols). Fish and crayfish tissues were analyzed for select pharmaceuticals, antimicrobial compounds, plastic components, and alkylphenols. Multiple biological endpoints were assessed in resident and caged fish to assess biological effects of exposure to CECs.

The occurrence of chemicals of emerging concern (CECs) and potential effects to aquatic biota in select tributaries of the St. Croix River in Minnesota and Wisconsin were measured. In 2011, treated wastewater effluent samples were collected from 22 sites in the St. Croix River Basin to determine total estrogenic activity. In 2012, wastewater effluent was collected at five select locations based on total estrogenicity and analyzed for CECs. In addition, surface water, bottom sediment, resident fish, and resident crayfish were collected upstream and downstream from effluent discharges. Water samples were analyzed for CECs (wastewater indicator compounds, steroid hormones, sterols, and pharmaceuticals). Bottom sediment samples were analyzed for a subset of CECs (wastewater indicator compounds, steroid hormones, and sterols). Fish and crayfish tissues were analyzed for select pharmaceuticals, antimicrobial compounds, plastic components, and alkylphenols. Multiple biological endpoints were assessed in resident and caged fish to assess biological effects of exposure to CECs.

We examined whether contaminants present in surface waters could be prioritized for further assessment by linking the presence of specific chemicals to gene expression changes in exposed fish. Fathead minnows were deployed in cages for 2, 4, or 8 days at three locations near two different waste water treatment plant discharge sites in the Saint Louis Bay, Duluth, MN and one upstream control site. The biological impact of 51 chemicals detected in the surface water was determined using biochemical endpoints, exposure activity ratios for biological and estrogenic responses; known chemical:gene interactions from biological pathways and knowledge bases, and analysis of the co-variance of ovary gene expression with surface water chemistry. Thirty-two chemicals were significantly linked by co-variance with expressed genes. No estrogenic impact on biochemical endpoints was observed in male or female minnows. However, bisphenol A was identified by chemical:gene co-variation as the most impactful chemical across the exposure sites. This was consistent with identification of estrogenic effects on gene expression, high exposure activity ratios across all test sites, and historical analysis of the area. Overall, this approach appears useful in examining the impacts of complex mixtures on fish and offers a potential route in linking chemical exposure to adverse outcomes that reduce population sustainability. This dataset is associated with the following publication: Perkins, E., T. Habib, B. Escalon, J. Cavallin, L. Thomas, M. Weberg, M. Hughes, K. Jensen, M. Kahl, D. Villeneuve, G. Ankley, and N. Garcia-Reyero. Prioritization of contaminants of emerging concern in wastewater treatment plant discharges using chemical: Gene interactions in caged fish. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 51(15): 8701-8712, (2017).

Concentrations of 127 organic chemicals measured in water samples collected from five locations in proximity to two municipal wastewater treatment plants in the St. Croix River basin, MN and WI, USA are included. Additionally, gene expression in the livers of fathead minnows exposed in situ to the site water for 12 d is included. Gene expression was analyzed by oligonucleotide microarray and raw data are accessible through the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO), accession number GSE81263. Additional analyses performed on those data, including construction of knowledge assembly models from comparison of the detected chemicals data with associated genes from the comparative toxicogenomics database, pathway and gene ontology enrichment analyses performed on the gene expression data, and richness and concordance statistics from a Reverse Causal Reasoning-based statistical approach are included. This dataset is associated with the following publication: Schroeder , A., D. Martinović-Weigelt, G. Ankley , K. Lee, N. Garcia-Reyero, E. Perkins, H. Schoenfuss, and D. Villeneuve. Prior knowledge-based approach for associating contaminants with biological effects: A case study in the St. Croix river basin, MN, WI, USA.. ENVIRONMENTAL POLLUTION. Elsevier Science Ltd, New York, NY, USA, 221: 427-436, (2017).

Concentrations of 127 organic chemicals measured in water samples collected from five locations in proximity to two municipal wastewater treatment plants in the St. Croix River basin, MN and WI, USA are included. Additionally, gene expression in the livers of fathead minnows exposed in situ to the site water for 12 d is included. Gene expression was analyzed by oligonucleotide microarray and raw data are accessible through the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO), accession number GSE81263. Additional analyses performed on those data, including construction of knowledge assembly models from comparison of the detected chemicals data with associated genes from the comparative toxicogenomics database, pathway and gene ontology enrichment analyses performed on the gene expression data, and richness and concordance statistics from a Reverse Causal Reasoning-based statistical approach are included. This dataset is associated with the following publication: Schroeder , A., D. Martinović-Weigelt, G. Ankley , K. Lee, N. Garcia-Reyero, E. Perkins, H. Schoenfuss, and D. Villeneuve. Prior knowledge-based approach for associating contaminants with biological effects: A case study in the St. Croix river basin, MN, WI, USA.. ENVIRONMENTAL POLLUTION. Elsevier Science Ltd, New York, NY, USA, 221: 427-436, (2017).