The present study highlights the utility of bioeffects-based monitoring in conjunction with analytical chemical measurements of surface waters on the Colorado River associated with a historically bioactive wastewater treatment plant effluent. Concurrent with chemical monitoring and in vitro bioactivity measurements, in situ caged fish systems were employed to evaluate the potential bioavailability of predicted biologically-active contaminants associated with ER, GR, and PPAR-associated activities. The present study compares the effects of a wastewater treatment plant facility upgrade on bioactive contaminant loading. This dataset is associated with the following publication: Cavallin, J., W. Battaglin, J. Beihoffer, B. Blackwell, P. Bradley, A. Cole, D. Ekman, R. Hofer, J. Kinsey, K. Keteles, D. Winkelman, and D. Villeneuve. Effects-Based Monitoring of Bioactive Chemicals Discharged to the Colorado River Before and After a Municipal Wastewater Treatment Plant Replacement. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 55(2): 974-984, (2021).

The datafile contains data associated with analytical chemistry, fish gene expression, in vitro bioassays, chemical prioritization, and Attagene data for surface water monitoring and caged fish studies on the South Platte River (Colorado, USA) in 2018 and 2019. This dataset is associated with the following publication: Cavallin, J., J. Beihoffer, B. Blackwell, A. Cole, D. Ekman, R. Hofer, A. Jastrow, J. Kinsey, K. Keteles, E. Maloney, J. Parman, D. Winkelman, and D. Villeneuve. Effects-based monitoring of bioactive compounds associated with municipal wastewater treatment plant effluent discharge to the South Platte River, Colorado, USA. ENVIRONMENTAL POLLUTION. Elsevier Science Ltd, New York, NY, USA, 289: 117928, (2021).

The datafile contains data associated with analytical chemistry, fish gene expression, in vitro bioassays, chemical prioritization, and Attagene data for surface water monitoring and caged fish studies on the South Platte River (Colorado, USA) in 2018 and 2019. This dataset is associated with the following publication: Cavallin, J., J. Beihoffer, B. Blackwell, A. Cole, D. Ekman, R. Hofer, A. Jastrow, J. Kinsey, K. Keteles, E. Maloney, J. Parman, D. Winkelman, and D. Villeneuve. Effects-based monitoring of bioactive compounds associated with municipal wastewater treatment plant effluent discharge to the South Platte River, Colorado, USA. ENVIRONMENTAL POLLUTION. Elsevier Science Ltd, New York, NY, USA, 289: 117928, (2021).

Concentrations of 127 organic chemicals measured in water samples collected from five locations in proximity to two municipal wastewater treatment plants in the St. Croix River basin, MN and WI, USA are included. Additionally, gene expression in the livers of fathead minnows exposed in situ to the site water for 12 d is included. Gene expression was analyzed by oligonucleotide microarray and raw data are accessible through the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO), accession number GSE81263. Additional analyses performed on those data, including construction of knowledge assembly models from comparison of the detected chemicals data with associated genes from the comparative toxicogenomics database, pathway and gene ontology enrichment analyses performed on the gene expression data, and richness and concordance statistics from a Reverse Causal Reasoning-based statistical approach are included. This dataset is associated with the following publication: Schroeder , A., D. Martinović-Weigelt, G. Ankley , K. Lee, N. Garcia-Reyero, E. Perkins, H. Schoenfuss, and D. Villeneuve. Prior knowledge-based approach for associating contaminants with biological effects: A case study in the St. Croix river basin, MN, WI, USA.. ENVIRONMENTAL POLLUTION. Elsevier Science Ltd, New York, NY, USA, 221: 427-436, (2017).

Concentrations of 127 organic chemicals measured in water samples collected from five locations in proximity to two municipal wastewater treatment plants in the St. Croix River basin, MN and WI, USA are included. Additionally, gene expression in the livers of fathead minnows exposed in situ to the site water for 12 d is included. Gene expression was analyzed by oligonucleotide microarray and raw data are accessible through the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO), accession number GSE81263. Additional analyses performed on those data, including construction of knowledge assembly models from comparison of the detected chemicals data with associated genes from the comparative toxicogenomics database, pathway and gene ontology enrichment analyses performed on the gene expression data, and richness and concordance statistics from a Reverse Causal Reasoning-based statistical approach are included. This dataset is associated with the following publication: Schroeder , A., D. Martinović-Weigelt, G. Ankley , K. Lee, N. Garcia-Reyero, E. Perkins, H. Schoenfuss, and D. Villeneuve. Prior knowledge-based approach for associating contaminants with biological effects: A case study in the St. Croix river basin, MN, WI, USA.. ENVIRONMENTAL POLLUTION. Elsevier Science Ltd, New York, NY, USA, 221: 427-436, (2017).

Water-borne contaminants were monitored in 69 tributaries of the Laurentian Great Lakes in 2010 and 2014 using semipermeable membrane devices (SPMDs), and polar organic chemical integrative samplers (POCIS). Analyses included 185 chemicals (143 detected) including PAHs, legacy and current-use pesticides, fire retardants, pharmaceuticals, fragrances, and others. Hazard quotients were calculated by dividing detected concentrations by biological effect concentrations reported in the ECOTOX Knowledgebase (Toxicity quotients, TQs) or ToxCast database (Exposure Activity Ratios, EARs). This dataset is associated with the following publication: Alvarez, D., S. Corsi, L. De Cicco, D. Villeneuve, and A. Baldwin. Identifying chemicals and mixtures of potential biological concern detected in passive samplers from Great Lakes tributaries using high-throughput data and biological pathways. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA,

Water-borne contaminants were monitored in 69 tributaries of the Laurentian Great Lakes in 2010 and 2014 using semipermeable membrane devices (SPMDs), and polar organic chemical integrative samplers (POCIS). Analyses included 185 chemicals (143 detected) including PAHs, legacy and current-use pesticides, fire retardants, pharmaceuticals, fragrances, and others. Hazard quotients were calculated by dividing detected concentrations by biological effect concentrations reported in the ECOTOX Knowledgebase (Toxicity quotients, TQs) or ToxCast database (Exposure Activity Ratios, EARs). This dataset is associated with the following publication: Alvarez, D., S. Corsi, L. De Cicco, D. Villeneuve, and A. Baldwin. Identifying chemicals and mixtures of potential biological concern detected in passive samplers from Great Lakes tributaries using high-throughput data and biological pathways. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA,

Water and sediment was collected to assess the occurrence of contaminants of emerging concern (CECs) in National Park waters of the northern Colorado Plateau, USA. CEC presence in water and sediment is reported for 21 sites in eight U.S. national parks in the northern Colorado Plateau region. From 2012 to 2016, at least one PPCP and/or WWI was detected at most sites on over half of sampling visits, indicating that CECs are not uncommon even in isolated areas. Maximum concentrations in this study were generally below available water quality benchmarks, sediment quality guidelines, and concentrations known to induce biological activity in vitro. C occurrence patterns and similarities between continuous and isolated flow locations suggest that direct contamination from individual visitors may also occur. While the data indicate there is little aquatic health risk associated with CECs at our sites, results demonstrate the ubiquity of CECs on the landscape and a continued need for public outreach concerning resource-use ethics and the potential effects of upstream development. This dataset is associated with the following publication: Weissinger, R., B. Blackwell, K. Keteles, W. Battaglin, and P. Bradley. Bioactive contaminants of emerging concern in national park waters of the northern Colorado plateau, USA. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 636: 910-918, (2018).

Water and sediment was collected to assess the occurrence of contaminants of emerging concern (CECs) in National Park waters of the northern Colorado Plateau, USA. CEC presence in water and sediment is reported for 21 sites in eight U.S. national parks in the northern Colorado Plateau region. From 2012 to 2016, at least one PPCP and/or WWI was detected at most sites on over half of sampling visits, indicating that CECs are not uncommon even in isolated areas. Maximum concentrations in this study were generally below available water quality benchmarks, sediment quality guidelines, and concentrations known to induce biological activity in vitro. C occurrence patterns and similarities between continuous and isolated flow locations suggest that direct contamination from individual visitors may also occur. While the data indicate there is little aquatic health risk associated with CECs at our sites, results demonstrate the ubiquity of CECs on the landscape and a continued need for public outreach concerning resource-use ethics and the potential effects of upstream development. This dataset is associated with the following publication: Weissinger, R., B. Blackwell, K. Keteles, W. Battaglin, and P. Bradley. Bioactive contaminants of emerging concern in national park waters of the northern Colorado plateau, USA. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 636: 910-918, (2018).