Data represent response variables from a series of mesocosm experiments to assess how estuarine macrophyte communities respond to nitrogen loading under two different thermal regimes. This dataset is associated with the following publication: Kaldy, J., C. Brown, W. Nelson, and M. Frazier. Macrophyte Community Response to Nitrogen Loading and Thermal Stressors in Rapidly Flushed Mesocosm Systems. JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY. Elsevier Science Ltd, New York, NY, USA, 497: 107-119, (2017).

fecal coliform data from the Oregon Dept. of Agriculture from 01/2006-12/2015 collected in Tillamook Bay, OR. This dataset is not publicly accessible because: This data is Oregon Dept. of Agriculture data and not EPA-generated data. It can be accessed through the following means: independent variables used in the model are available on public websites - summarized in table 1 of the paper and associated text. Format: This is Oregon Department of Agriculture data. It is not available to provide a link or file. This dataset is associated with the following publication: Zimmer-Faust, A., C. Brown, and A. Manderson. Statistical models of fecal coliform levels in Pacific Northwest estuaries for improved shellfish harvest area closure decision making. MARINE POLLUTION BULLETIN. Elsevier Science Ltd, New York, NY, USA, 137: 360-369, (2018).

These data have been collected as part of a cooperative project between the U.S. Geological Survey (USGS) and the U.S Environmental Protection Agency. This project began in 2016 and consisted of a mesocosm experiment quantifying short term responses by a transplanted natural diatom dominant algae community exposed to gradients of nitrogen, phosphorous, and a mixture of both nitrogen and phosphorus. The data consist of water quality parameters such as temperature and pH as well as aqueous nutrient concentrations and algae responses, including chlorophyll a concentrations, algae dry mass, algae ash-free dry mass, and intra-cellular phosphorus and nitrogen in algae. Algae growth rates were estimated from these data and are meant to be used with water-quality models; the results of this investigation can be found in the manuscript associated with this data release (Schmidt and others 2019).

These data have been collected as part of a cooperative project between the U.S. Geological Survey (USGS) and the U.S Environmental Protection Agency. This project began in 2016 and consisted of a mesocosm experiment quantifying short term responses by a transplanted natural diatom dominant algae community exposed to gradients of nitrogen, phosphorous, and a mixture of both nitrogen and phosphorus. The data consist of water quality parameters such as temperature and pH as well as aqueous nutrient concentrations and algae responses, including chlorophyll a concentrations, algae dry mass, algae ash-free dry mass, and intra-cellular phosphorus and nitrogen in algae. Algae growth rates were estimated from these data and are meant to be used with water-quality models; the results of this investigation can be found in the manuscript associated with this data release (Schmidt and others 2019).

Data used to generate statistical analysis in the manuscript "Carbon limitation in response to nutrient loading in an eelgrass mesocosm: influence of water residence time on eutrophication expression" by Kaldy, Brown and Pacella. This dataset is associated with the following publication: Kaldy, J., C. Brown, and S. Pacella. Carbon limitation in response to nutrient loading in an eelgrass mesocosm: influence of water residence time.. MARINE ECOLOGY PROGRESS SERIES. Inter-Research, Luhe, GERMANY, 689: 1-17, (2022).

An interdisciplinary and multiagency study was undertaken to study harmful algal bloom (HAB) dynamics on the Caloosahatchee River, which drains to the west from Lake Okeechobee into the Gulf of Mexico. Algae and cyanobacteria play crucial roles in aquatic ecosystems, but under favorable environmental conditions certain taxa may experience population growth booms resulting in HABs. Four independent mesocosm experiments were conducted in September 2019, June 2020, September 2020, and February 2021 to capture natural temporal variability in water quality and phytoplankton assemblages. The experimental installation consisted of three floating metal cradles each containing four fiberglass chambers filled with native river water. The twelve chambers were assigned to one of four treatments: phosphate (P), nitrate (N), ammonium (A), or control (C) – each with 3 replicates. The P, N, and A treatment chambers were enriched by applying a concentrated dosing solution of either dibasic dodecahydrate sodium phosphate, anhydrous sodium nitrate, or liquid ammonium hydroxide respectively to elevate concentrations above ambient levels. The mesocosms were treated and sampled over a 72-hour period (Time 0, Time 24, Time 48, Time 72). This dataset contains phytoplankton absolute and relative abundance measures at the genus level as natural units (NU/mL), cell densities (cells/mL), and biovolumes (um3/mL).

An interdisciplinary and multiagency study was undertaken to study harmful algal bloom (HAB) dynamics on the Caloosahatchee River, which drains to the west from Lake Okeechobee into the Gulf of Mexico. Algae and cyanobacteria play crucial roles in aquatic ecosystems, but under favorable environmental conditions certain taxa may experience population growth booms resulting in HABs. Four independent mesocosm experiments were conducted in September 2019, June 2020, September 2020, and February 2021 to capture natural temporal variability in water quality and phytoplankton assemblages. The experimental installation consisted of three floating metal cradles each containing four fiberglass chambers filled with native river water. The twelve chambers were assigned to one of four treatments: phosphate (P), nitrate (N), ammonium (A), or control (C) – each with 3 replicates. The P, N, and A treatment chambers were enriched by applying a concentrated dosing solution of either dibasic dodecahydrate sodium phosphate, anhydrous sodium nitrate, or liquid ammonium hydroxide respectively to elevate concentrations above ambient levels. The mesocosms were treated and sampled over a 72-hour period (Time 0, Time 24, Time 48, Time 72). This dataset contains phytoplankton absolute and relative abundance measures at the genus level as natural units (NU/mL), cell densities (cells/mL), and biovolumes (um3/mL).

To address how phytoplankton in the Great Lakes respond to macro- and micronutrients, we conducted a bottle incubation enrichment experiment using water collected from blooming (Maumee Bay and Fox River) and non-blooming sites (Detroit River and Ford River) in Lakes Erie and Michigan, respectively, during late summer. Surface water from these locations was collected and taken to Kent State University either via overnight shipping (Lake Michigan sites) or driven directly after collection (Lake Erie sites). Chlorophyll a (an index of overall biomass), community composition and toxicity were all measured as responses to treatments of labile inorganic nitrogen (N), phosphorus (P) and a mixture of micronutrients (chemical symbols: Fe, Mn, Mo, Ni, Zn).

The data describe greenhouse gas (GHG) emissions, dissolved GHG concentrations, and mesocosm conditions (vegetation, hydrologic phases, ambient conditions) of a controlled, laboratory study.