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Minimum Inhibitory Concentration (MIC) data for third generation cephalosporin resistant E. coli and extended spectrum beta-lactamase producing Enterobacteriaceae from feedlot cattle
,The data presents the antimicrobial susceptibility testing results in three separate files: 1) third generation cephalosporin resistant E. coli isolates obtained on cefotaxime supplemented media; 2) extended spectrum beta-lactamase (ESBL) producing E. coli, and 3) ESBL-producing Klebsiella, Enterobacter and Citrobacter species obtained on chromogenic media. The data was generated as part of a research project that evaluated the impact of tylosin supplementation of feedlot cattle on the dynamics of antimicrobial resistant fecal bacteria. The study was a longitudinal design with periodic sampling of fecal samples from individual animals over the entire feeding period. Two publications from the project, one describing the study design in detail, and the other specifically reporting on these data, are linked to the database.,Resources in this dataset:,
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Agriculturally sourced multidrug-resistant Escherichia coli for use as control strains
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,Global efforts emphasize the need to incorporate human, animal, and environmental components into coordinated and standardized surveillance programs. This requires microbiological methods be standardized, including using standardized positive and negative bacterial control strains. There is a need to provide bacterial control strains that are sourced from and relevant for environmental surveillance efforts.,· Two control strains are available in support of environmental antibiotic resistance surveillance efforts.,· Escherichia coli ARS-C301 is extended-spectrum β-lactamase positive and tetracycline resistant.,· E. coli ARS-C101 is cefotaxime and tetracycline susceptible and serves as a negative control.,· Strains are DNA sequenced, and deposited in publicly available culture collections.,Strain Availability:,ARS-C101 (negative control): WGS is available from NCBI, accession number PRJNA1003888, BioSample B-65681; Isolate is available from the ARS Culture Collection https://nrrl.ncaur.usda.gov/ (Accession number: B-65681); and from ATCC (isolate ID BAA-3340).,ARS-C301 (positive control): WGS is available from NCBI, accession number PRJNA1003888, BioSample SAMN36910816; Isolate is available from the ARS Culture Collection https://nrrl.ncaur.usda.gov/ (Accession number: B-65682); from ATCC (isolate ID BAA-3341); and from Microbiologics as a quantitative pellet, via a custom order (contact industrial microbiology QC products regional sales manager by email requesting a quote for ARS-C301 https://www.microbiologics.com/contact-us).,
Persistence of antibiotic resistance genes in beef cattle backgrounding environment after cessation of operation
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,Datasheet for the abundance of total bacteria, gram positive indicator bacteria, horizontal gene transfer indicator genes and antibiotic resistance genes in the soil measured over 3 years.,,
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