These data contain sample information and locality and quantitative polymerase chain reaction (qPCR) results from extraction and testing of individual tape strips within Passive Environmental Samplers (PES). Samplers were placed at 5 locations on Hawaii Island between 2016 and 2017.

These data contain sample information, locality and quantitative polymerase chain reaction (qPCR) results from extraction and testing of individual tape strips within Passive Environmental Samplers (PES). Samplers were placed at 12 sites on Hawaii Island between 2016 and 2017.

These data contain sample information, locality and quantitative polymerase chain reaction (qPCR) results from extraction and testing of individual tape strips within Passive Environmental Samplers (PES). Samplers were placed at 12 sites on Hawaii Island between 2016 and 2017.

These data contain the raw quantitative polymerase chain reaction (qPCR) results for all Ceratocystis lukuohia and huliohia testing of ohia trees, soil, and seedlings.

These data contain the raw quantitative polymerase chain reaction (qPCR) results for all Ceratocystis lukuohia and huliohia testing of environmental DNA (eDNA) collected in the wind column using Passive Environmental Samplers (PES).

The data contain the raw quantitative polymerase chain reaction (qPCR) results after pre-amplification by polymerase chain reaction for all Ceracystis lukuohia and huliohia testing of environmental DNA (eDNA) collected in Passive Environmental Samplers (PES).

These data provide geographic coordinates (latitude and longitude) and elevation of Passive Environmental Samplers (PES) that were deployed to detect airborne propagules of the invasive fungi that cause Rapid Ohia Death (Ceratocystis lukuohia and C. huliohia) and Ohia Rust (Austropuccina psidii)

These data include metadata and associated data files associated with the manuscript, "Economical Environmental Sampler Designs for Detecting Airborne Spread of Fungi Responsible for Rapid Ohia Death." These data include a total of 8 datasets used for both controlled and field studies evaluating the use of Active (with battery operated fan) and Passive (dependent on wind) USGS Environmental Samplers on Hawaii Island between 2016-2018. Samplers were operated under controlled laboratory and field conditions with a commercial sampler (Rotorod Model 20) to compare efficacy in capturing synthetic polyethylene spheres (12 - 160 µm in diameter) and also Xyleborus spp. boring dust (frass) known to contain the fungi responsible for Rapid Ohia Death (Ceratocystis lukuohia and C. huliohia). Samplers were also tested at a field site with confirmed ROD mortality to demonstrate their ability to detect Ceratocystis DNA in beetle frass from infected trees and wood chips associated with tree felling under a range of environmental conditions. Data files consists of particle counts, particle measurements, and results of qPCR (quantitative polymerase chain reaction) tests to determine presence or absence of Ceratocystis DNA on tape strips that were exposed in samplers and tape strips that were spiked with known numbers of Ceratocystis spores to determine sensitivity of the methodology.

Bacillus species and B. anthracis presence/absence data were determined in 4,770 soil samples collected across the contiguous United States, in cooperation with the U.S. Environmental Protection Agency (EPA). Polymerase Chain Reaction (PCR) data for Bacillus species and B. anthracis rpoB gene PCR amplicon detection were reported as non-detect (n), low (l), medium (m), and high (h). Results for both pag and lef genes of the pX01 plasmid were reported by the University of South Florida's Center for Biological Defense. This data was recorded as negative or positive for each of the genes and included the following combinations: neg/neg, pos/neg, neg/pos, and pos/pos. Data for the pX02 plasmid were recorded as negative (neg) or positive (pos).

This data release includes metadata and tabular datasets that document (1) Austropuccina, Ceratocystis and Myrtaceae qPCR (quantitative polymerase chain reaction) DNA detections in Passive Environmental Samplers (PES), (2) wind speed, wind gust speed, and wind direction measurements collected at two sites in the Kahuku Unit of Hawai'i Volcanoes National Park (HAVO) where paired PES were located, (3) localities, sites and elevations where PES were located, and (4) Genbank accession numbers for Austropuccinia and Ceratocystis DNA sequences amplified from samples collected in a subset of PES. These raw data were analyzed and reported in the manuscript "Environmental Monitoring for Invasive Fungal Pathogens of ʽŌhiʽa (Metrosideros polymorpha) on the Island of Hawaiʽi".