These data contain the raw quantitative polymerase chain reaction (qPCR) results for all Ceratocystis lukuohia and huliohia testing of environmental DNA (eDNA) collected in the wind column using Passive Environmental Samplers (PES).

The data contain the raw quantitative polymerase chain reaction (qPCR) results after pre-amplification by polymerase chain reaction for all Ceracystis lukuohia and huliohia testing of environmental DNA (eDNA) collected in Passive Environmental Samplers (PES).

These data contain sample information and locality and quantitative polymerase chain reaction (qPCR) results from extraction and testing of individual tape strips within Passive Environmental Samplers (PES). Samplers were placed at 5 locations on Hawaii Island between 2016 and 2017.

These data contain the raw quantitative polymerase chain reaction (qPCR) results for all Ceratocystis lukuohia and huliohia testing of environmental DNA (eDNA) collected in the wind column using Passive Environmental Samplers (PES).

We placed 23 USGS Passive Environmental Samplers (PES) in a grid that spanned the outbreak and monitored for airborne frass containing Ceratocystis lukuohia and C. huliohia.These data include the latitude and longitude of PES locations.

Hawaiʹi’s most widespread native tree, ʹōhiʹa lehua (Metrosideros polymorpha), has been dying across large areas of Hawaiʹi Island mainly due to two fungal pathogens (Ceratocystis lukuohia and Ceratocystis huliohia) that cause a disease collectively known as Rapid ʹŌhiʹa Death (ROD). Here we examine patterns of positive detections of C. lukuohia as it has been linked to the larger mortality events across Hawaiʹi Island. Our analysis compares the environmental range of C. lukuohia and its spread over time through the known climatic range and distribution of ʹōhiʹa. This data release consists of two rasters, one containing the projected suitability for C.lukuohia and another consisting of modeled presence/absence across the main Hawaiian Islands under current climatic conditions. This distribution model for C. lukuohia was generated using maxent using methods described in Fortini et. al 2019 (Forest Ecology and Management). Full citation is listed in the larger work section of this XML file.

Rapid ʻŌhiʻa Death is a fatal disease caused by Ceratocystis lukuohia and C. huliohia that are decimating Hawaiʻiʻs keystone trees species, ʻōhiʻa (Metrosideros polymorpha). We developed practical and effective decontamination practices for both cultured C. lukuohia and C. huliohia as well as Ceratocystis spores embedded in ambrosia beetle frass (fine particles of macerated wood or boring dust, beetle parts, and feces). This data release includes data and metadata containing decontamination results of ambrosia beetle (Coleoptera: Scolytinae) frass contaminated with Ceratocystis lukuohia propagules using various laboratory and household disinfectants.

These data contain the study site locations and elevation information where bolts (tree stem sections) infected with Ceratocystis lukuohia and/or C. huliohia were collected on Hawai'i Island.

Hawaiʹi’s most widespread native tree, ʹōhiʹa lehua (Metrosideros polymorpha), has been dying across large areas of Hawaiʹi Island mainly due to two fungal pathogens (Ceratocystis lukuohia and Ceratocystis huliohia) that cause a disease collectively known as Rapid ʹŌhiʹa Death (ROD). Here we examine patterns of positive detections of C. lukuohia as it has been linked to the larger mortality events across Hawaiʹi Island. Our analysis compares the environmental range of C. lukuohia and its spread over time through the known climatic range and distribution of ʹōhiʹa. This data set is a georeferenced raster file, containing the projected suitability for C.lukuohia across the main Hawaiian Islands using climatic variables that varied consistently with C. lukuohia prevalence (Mean annual precipication and minimum temperature of coldest month) at 500 meter resolution. This C. lukuohia habitat suitability was generated using maxent using methods described in Fortini et. al 2019 (Forest Ecology and Management). Full citation is listed in the larger work section of this XML file.

Rapid ʽŌhiʽa Death (ROD) currently threatens ōhiʽa lehua (Metrosideros polymorpha) on Hawaiʽi Island. First identified in Puna in 2014, the disease has now spread island wide. Besides direct sampling of trees, environmental sampling could serve as an easier and broader strategy to detect Ceratocystis spp., the fungi causing ROD. Environmental sampling could also help monitor the effect of felling ROD infected trees. We developed Passive and Active Environmental Samplers and deployed them at a property in Puna, where both C. lukuohia, and C. huliohia had been detected, and where the land owner practiced the management method of felling infected trees. We set up 2 Active Environmental Samplers (modified mosquito traps connected to a battery that uses a fan to continuously draw in air) and 3 Passive Environmental Samplers (uses a vane to move in the direction of the wind without the use of electricity) from July 12th to October 25th, 2016. The Active Samplers contained one slide (1 replicate) each, while the Passive Samplers contained 4 slides (4 replicates) each. Samplers were located in the lawn (2 Active, 1 Passive), next to a rainwater catchment tank (1 Passive), and next to a small shed (1 Passive). The dataset contains a list of sampling weeks and their start and end dates, and quantitative polymerase chain reaction (qPCR) results for individual slides that were collected from Active and Passive Samplers located at the Lawn, Tank and Shed sites. Samples were tested twice for Ceratocystis lukuohia, Ceratocystis huliohia, and Metrosideros polymorpha DNA after extraction with a Machery Nagel Plant II Extraction Kit and again after DNA was concentrated by ethanol precipitation. Positive qPCR test results are presented as quantitation cycle (Cq) in which fluorescence is detected for Ceratocystis lukuohia, Ceratocystis huliohia, and Metrosideros polymorpha DNA for each individual slide and number of replicates that were positive out of three for C. lukuohia and C. huliohia and out of six for M. polymorpha. Lines that are blank in columns for Cq values reflect negative test results. When Cq values have more than one replicate for a test, the reported Cq value represents the average of all positive replicates.