We placed 23 USGS Passive Environmental Samplers (PES) in a grid that spanned the outbreak and monitored for airborne frass containing Ceratocystis lukuohia and C. huliohia.These data include the latitude and longitude of PES locations.

These data provide geographic coordinates (latitude and longitude) and elevation of Passive Environmental Samplers (PES) that were deployed to detect airborne propagules of the invasive fungi that cause Rapid Ohia Death (Ceratocystis lukuohia and C. huliohia) and Ohia Rust (Austropuccina psidii)

These data contain the raw quantitative polymerase chain reaction (qPCR) results for all Ceratocystis lukuohia and huliohia testing of environmental DNA (eDNA) collected in the wind column using Passive Environmental Samplers (PES).

These data include metadata and associated data files associated with the manuscript, "Economical Environmental Sampler Designs for Detecting Airborne Spread of Fungi Responsible for Rapid Ohia Death." These data include a total of 8 datasets used for both controlled and field studies evaluating the use of Active (with battery operated fan) and Passive (dependent on wind) USGS Environmental Samplers on Hawaii Island between 2016-2018. Samplers were operated under controlled laboratory and field conditions with a commercial sampler (Rotorod Model 20) to compare efficacy in capturing synthetic polyethylene spheres (12 - 160 µm in diameter) and also Xyleborus spp. boring dust (frass) known to contain the fungi responsible for Rapid Ohia Death (Ceratocystis lukuohia and C. huliohia). Samplers were also tested at a field site with confirmed ROD mortality to demonstrate their ability to detect Ceratocystis DNA in beetle frass from infected trees and wood chips associated with tree felling under a range of environmental conditions. Data files consists of particle counts, particle measurements, and results of qPCR (quantitative polymerase chain reaction) tests to determine presence or absence of Ceratocystis DNA on tape strips that were exposed in samplers and tape strips that were spiked with known numbers of Ceratocystis spores to determine sensitivity of the methodology.

This data release includes metadata and tabular datasets that document (1) Ceratocystis qPCR DNA detections in Passive Environmental Samplers (PES), (2) Ceratocystis qPCR DNA detections after pre-amplification, (3) wood and frass particle counts detected in PES (4) location of individual PES within the study area (5) ambrosia beetle gallery counts on felled ʻōhiʻa (6) ambrosia beetle gallery counts on insecticide treated bolts and (7) ambrosia beetles trapped in cross-vein panel traps. Data were used in the analysis of the manuscript "Effectiveness of Rapid ʻŌhiʻa Death management strategies at a focal disease outbreak on Hawaiʻi Island."

These data contain the study site locations and elevation information where bolts (tree stem sections) infected with Ceratocystis lukuohia and/or C. huliohia were collected on Hawai'i Island.

We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard® samplers, but are more economical and require less maintenance than commercial devices. We compared our two new samplers to Rotorod® samplers using Xyleborus spp. boring dust (frass) known to contain fungi responsible for Rapid Ohia Death. The comparison was done in a large outdoor field cage to determine relative effectiveness of the three samplers for capturing windblown boring dust. The dataset contains counts of boring dust particles that were captured by the three types of samples over the course of twelve trials.

This EnviroAtlas dataset includes a number of Discharge Monitoring Report (DMR) metrics summarized by watershed for a given year (https://echo.epa.gov/help/loading-tool/watershed-statistics-help). These metrics include the number of facilities and wastewater discharges located within watersheds according to the Integrated Compliance Information System National Pollutant Discharge Elimination System (ICIS-NPDES), to track the permit compliance and enforcement status of facilities regulated by the NPDES under the Clean Water Act (https://echo.epa.gov/tools/data-downloads/icis-npdes-download-summary). This dataset was produced by the US EPA to support research and online mapping activities related to EnviroAtlas. EnviroAtlas (https://www.epa.gov/enviroatlas) allows the user to interact with a web-based, easy-to-use, mapping application to view and analyze multiple ecosystem services for the contiguous United States. The dataset is available as downloadable data (https://edg.epa.gov/data/Public/ORD/EnviroAtlas) or as an EnviroAtlas map service. Additional descriptive information about each attribute in this dataset can be found in its associated EnviroAtlas Fact Sheet (https://www.epa.gov/enviroatlas/enviroatlas-fact-sheets).

This EnviroAtlas dataset includes a number of Discharge Monitoring Report (DMR) metrics summarized by watershed for a given year (https://echo.epa.gov/help/loading-tool/watershed-statistics-help). These metrics include the number of facilities and wastewater discharges located within watersheds according to the Integrated Compliance Information System National Pollutant Discharge Elimination System (ICIS-NPDES), to track the permit compliance and enforcement status of facilities regulated by the NPDES under the Clean Water Act (https://echo.epa.gov/tools/data-downloads/icis-npdes-download-summary). This dataset was produced by the US EPA to support research and online mapping activities related to EnviroAtlas. EnviroAtlas (https://www.epa.gov/enviroatlas) allows the user to interact with a web-based, easy-to-use, mapping application to view and analyze multiple ecosystem services for the contiguous United States. The dataset is available as downloadable data (https://edg.epa.gov/data/Public/ORD/EnviroAtlas) or as an EnviroAtlas map service. Additional descriptive information about each attribute in this dataset can be found in its associated EnviroAtlas Fact Sheet (https://www.epa.gov/enviroatlas/enviroatlas-fact-sheets).

This EnviroAtlas dataset includes a number of Discharge Monitoring Report (DMR) metrics summarized by watershed for a given year (https://echo.epa.gov/help/loading-tool/watershed-statistics-help). These metrics include the number of facilities and wastewater discharges located within watersheds according to the Integrated Compliance Information System National Pollutant Discharge Elimination System (ICIS-NPDES), to track the permit compliance and enforcement status of facilities regulated by the NPDES under the Clean Water Act (https://echo.epa.gov/tools/data-downloads/icis-npdes-download-summary). This dataset was produced by the US EPA to support research and online mapping activities related to EnviroAtlas. EnviroAtlas (https://www.epa.gov/enviroatlas) allows the user to interact with a web-based, easy-to-use, mapping application to view and analyze multiple ecosystem services for the contiguous United States. The dataset is available as downloadable data (https://edg.epa.gov/data/Public/ORD/EnviroAtlas) or as an EnviroAtlas map service. Additional descriptive information about each attribute in this dataset can be found in its associated EnviroAtlas Fact Sheet (https://www.epa.gov/enviroatlas/enviroatlas-fact-sheets).