These data contain the raw quantitative polymerase chain reaction (qPCR) results for all Ceratocystis lukuohia and huliohia testing of environmental DNA (eDNA) collected in the wind column using Passive Environmental Samplers (PES).

These data contain the raw quantitative polymerase chain reaction (qPCR) results for all Ceratocystis lukuohia and huliohia testing of environmental DNA (eDNA) collected in the wind column using Passive Environmental Samplers (PES).

These data contain sample information, locality and quantitative polymerase chain reaction (qPCR) results from extraction and testing of individual tape strips within Passive Environmental Samplers (PES). Samplers were placed at 12 sites on Hawaii Island between 2016 and 2017.

These data contain sample information and locality and quantitative polymerase chain reaction (qPCR) results from extraction and testing of individual tape strips within Passive Environmental Samplers (PES). Samplers were placed at 5 locations on Hawaii Island between 2016 and 2017.

We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard® samplers, but are more economical and require less maintenance than commercial devices. We compared our two new samplers to Rotorod® samplers using Xyleborus spp. boring dust known to contain ROD causing pathogens. The comparison was done in a large outdoor field cage to determine relative effectiveness of the three samplers for capturing windblown boring dust. The dataset contains results of quantitative PCR (qPCR) tests to determine presence of Ceratocystis lukuohia, Ceratocystis huliohia, and Metrosideros polymorpha DNA in the samplers after release of boring dust over the course of twelve trials where position of samplers was rotated.

These data were collected as part of a voluntary initiative to create a White-Nose Syndrome Diagnostic Laboratory Network among laboratories participating in research and surveillance for Pseudogymonascus destructans (Pd) - the fungal pathogen causing White-Nose Syndrome in bats. Pd_qPCR_InterlaboratoryLODdata.xlsx is raw qPCR data from multiple laboratories running serial dilutions of Pd gBlock in known concentrations for the collectively used Muller (2013) Pd qPCR assay. Pd_qPCR_InterlaboratoryResults_LOD.xlsx contains the data output for each laboratory from running a generic LOD/LOQ calculator script. the generic LOD/LOQ calculator script is available at:https://github.com/cmerkes/qPCR_LOD_Calc. Pd_qPCR_InterlaboratoryPTResults_PanelData.xlsx contains the raw qPCR data from multiple laboratories running blinded samples spiked with known concentrations of Pd conidia. Each sample was extracted once and run in triplicate using the Muller (2013) assay. Pd_qPCR_InterlaboratoryPTResults_PanelResults contains the results of the blinded samples in each laboratory panel as both qPCR Ct values per replicate, and final overall sample result according to the WNS Case Definition. Pd_qPCR_InterlaboratoryPTResults_Standards.xlsx contains the results of the standard curves run by each laboratory in conjunction with the blinded sample panel.

The dataset contains qualitative detection and quantitative results of Esox Lucius DNA in environmental (e)DNA water samples collected from Miller Creek, Alaska, and analyzed with quantitative PCR.The dataset contains qualitative detection and quantitative results of Esox Lucius DNA in environmental (e)DNA water samples collected from Miller Creek, Alaska, and analyzed with quantitative PCR.

We placed 23 USGS Passive Environmental Samplers (PES) in a grid that spanned the outbreak and monitored for airborne frass containing Ceratocystis lukuohia and C. huliohia.These data include the latitude and longitude of PES locations.

This dataset contains quantitative results from, and covariate attributes of, environmental DNA water samples collected across multiple rivers in 2023. Samples were analyzed for eDNA of round goby in the Hudson River (NY), westslope cutthroat trout (Oncorhynchus clarkii lewisi) in Cherry Creek (MT), lake trout (Salvelinus namaycush) and Spectaclecase mussels (Cumberlandia monodonta) in Big Piney River (MO), and rainbow trout (O. mykiss) and Western Pearlshell (Margaritifera falcata) in Loggers Creek (ID).

This data shows the quantification cycle at which fluorescence signals crossed a threshold fluorescence for samples analyzed as part of controlled experiments to determine whether contaminating DNA is present in samples under varying experimental conditions.