Sediment was dosed with six highly hydrophobic chemicals with estimated log Kows ranging up to 18.3. Sediment bioaccumulation tests with Lumbriculus variegatus were performed with the dosed sediment. The attached dataset contains the concentrations in the organisms from the uptake and elimination portions of the test. and supporting data, e.g. lipid contents and weight change for the organisms. This dataset is associated with the following publication: Burkhard, L., T. Lahren, T. Highland, R. Hockett, D. Mount, and T. Norberg-King. Bioaccumulation of highly hydrophobic chemicals by Lumbriculus variegatus. ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY. Springer, New York, NY, USA, 76(1): 129-141, (2019).

Results from sediment bioaccumulation tests with Lumbriculus variegatus with evaluating the effects of organism loading density. This dataset is associated with the following publication: Burkhard , L., D. Hubin-Barrows, N. Billa, T. Highland , R. Hockett , D. Mount , and T. Norberg-King. Sediment Bioaccumulation Test with Lumbriculus variegatus: Effects of Organism Loading. ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY. Springer, New York, NY, USA, 71(7): 70-77, (2016).

- Concentration of TBPH and TBMEHP in sediment - Weigh and lipid contents of Lumbriculus variegatus over time - Concentrations in Lumbriculus variegatus of TBPH and TBMEPH, and L. variegatus lipid contents - Chemical Concentrations in Method Blanks. This dataset is associated with the following publication: Burkhard, L., T. Lahren, T. Highland, R. Hockett, D. Mount, and T. Norberg-King. Bioaccumulation of bis-(2-ethylhexyl)-3,4,5,6-tetrabromophthalate and mono-(2-ethylhexyl)-3,4,5,6-tetrabromophthalate by Lumbriculus variegatus. ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY. Springer, New York, NY, USA, 80: 579–586, (2021).

This paper describes the results of an international ring trial (round robin study) of in vitro methods used to measure chemical biotransformation in fish. Researchers at six laboratories in the U.S. and Europe evaluated six test chemicals (cyclohexyl salicylate, fenthion, 4-n-nonylphenol, deltamethrin, methoxychlor, and pyrene) using two in vitro systems (liver S9 fraction and isolated hepatocytes) derived from rainbow trout livers. This information was then evaluated to quantitatively characterize assay reliability (repeatability and reproducibility) and evaluate potential user bias associated with the methods. Additional calculations were performed using established models to estimate in vivo levels of hepatic clearance and predict the effects on chemical biotransformation on chemical bioaccumulation. The Science Hub dataset includes all information presented in the paper as figures and tables including, as appropriate, individual values used to calculate reported means and standard deviations. Also included are all datasets provided as Supplementary Data. This dataset is associated with the following publication: Nichols, J., K. Fay, M.J. Bernhard, I. Bischof, J. Davis, M. Halder, J. Hu, K. Johanning, H. Laue, D. Nabb, C. Schlechtriem, H. Segner, J. Swintek, J. Weeks, and M. Embry. Reliability of In Vitro methods used to measure intrinsic clearance of hydrophobic organic chemicals by rainbow trout: Results of an international ring trial. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 164(2): 563-575, (2018).

This paper describes the results of an international ring trial (round robin study) of in vitro methods used to measure chemical biotransformation in fish. Researchers at six laboratories in the U.S. and Europe evaluated six test chemicals (cyclohexyl salicylate, fenthion, 4-n-nonylphenol, deltamethrin, methoxychlor, and pyrene) using two in vitro systems (liver S9 fraction and isolated hepatocytes) derived from rainbow trout livers. This information was then evaluated to quantitatively characterize assay reliability (repeatability and reproducibility) and evaluate potential user bias associated with the methods. Additional calculations were performed using established models to estimate in vivo levels of hepatic clearance and predict the effects on chemical biotransformation on chemical bioaccumulation. The Science Hub dataset includes all information presented in the paper as figures and tables including, as appropriate, individual values used to calculate reported means and standard deviations. Also included are all datasets provided as Supplementary Data. This dataset is associated with the following publication: Nichols, J., K. Fay, M.J. Bernhard, I. Bischof, J. Davis, M. Halder, J. Hu, K. Johanning, H. Laue, D. Nabb, C. Schlechtriem, H. Segner, J. Swintek, J. Weeks, and M. Embry. Reliability of In Vitro methods used to measure intrinsic clearance of hydrophobic organic chemicals by rainbow trout: Results of an international ring trial. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 164(2): 563-575, (2018).

The data consists of the responses (survival, growth, and/or reproduction) of test organisms were determined in six concentrations of toxicants in 7-day toxicity tests or in four different feeding rates in 7-day feeding experiments. Specifically we evaluated the sensitivity of 2 mussel species (Villosa constricta and Lampsilis siliquoidea) and P. promelas and C. dubia using effluents in 7-d exposures. We then refined the method by determining the best feeding rate of algal mixture for 1-, 2-, and 3-wk-old L. siliquoidea in a 7-d feeding experiment, and using derived optimal feeding rates to assess the sensitivity of the 3 ages of juveniles in a 7-d NaCl test. Finally, we conducted an interlaboratory study among 13 laboratories to evaluate the performance of a 7-d NaCl test with L. siliquoidea.

The data release includes data from four studies: (1) toxicity of a permitted effluent, which entered the Deep Fork River (DFR), Oklahoma, USA, to a unionid mussel (Lampsilis siliquoidea) and to 2 standard test species (cladoceran Ceriodaphnia dubia; and fathead minnow Pimephales promelas) in short-term 7-d effluent tests; (2) relative sensitivities of the 3 species to potassium (K), an elevated major ion in the effluent, using 7-d toxicity tests with KCl spiked into a DFR upstream reference water; (3) potential influences of background water characteristics on the acute K toxicity to the mussel (96-h exposures) and cladoceran (48-h exposure) in 4 reconstituted waters that mimicked the hardness and ionic composition ranges of the DFR; and (4) potential influence of temperature on acute K toxicity to the mussel. Water quality, survival, growth, and reproduction endpoints are reported.

Detailed data and analysis supporting manuscript Dietary uptake of highly hydrophobic chemicals by rainbow trout. This dataset is associated with the following publication: Burkhard, L., T. Lahren, K. Hanson, A. Kasparek, and D. Mount. Dietary Uptake of Highly Hydrophobic Chemicals by Rainbow Trout (Oncorhynchus Mykiss). ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY. Springer, New York, NY, USA, 85(4): 390-403, (2023).

This data collection consists of raw luminescence data, measured as relative luminesence units (RLUs), from the tropical bioluminsencent bacterium Vibrio azureus exposed to the reference toxicants, copper and zinc. V. azureus cells were exposed to various different concentrations of both toxicants over acute (15, 30 min) and chronic (>24 h) exposure periods. In addition, luminescence data from the industry standard bacterial species, Aliivibrio fischeri, is included to benchmark the new bacterial toxicity assay. For full methodological and analytical details please refer to the full research publication, Luter et al. (in review) its supplementary materials and the associated GitHub repository (https://github.com/open-AIMS/Lum31-tox-assays.git).