Molecular detection of Tetracapsuloides bryosalmonae DNA from fish tissues and environmental DNA water samples collected throughout the Inter-mountain West, USA from 2011 to 2019

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This dataset contains molecular diagnostic results of the myxozoan parasite Tetracapsuloides byrosalmonae in fish tissue collected from the inter-mountain West, USA.

Quantitative PCR results for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples

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A probe-based quantitative real-time PCR assay was developed to detect Tetracapsuloides bryosalmonae, which causes Proliferative Kidney Disease in salmonid fish, in kidney tissue and environmental DNA (eDNA) water samples. We present data that was used to determine assay sensitivity, specificity and to define the limits of detection and quantification.

Conventional PCR results for detecting Tetracapsuloides bryosalmonae in fish tissue and water samples

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We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.

Simple sequence repeats (SSRs) of Tetracapsuloides bryosalmonae parasites in kidney tissue collected from various water bodies in Montana, USA from 2016 to 2019

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This dataset contains simple sequence repeat genotypes from Tetracapsuloides bryosalmonae infecting the tissue of fish collected from various locations throughout western and central Montana, USA.

Spatial and temporal survey of waterborne myxozoan parasites in the Lake Sammamish watershed, Washington, from 2019 - 2020

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There is a fundamental knowledge gap on the distribution, prevalence, intensity, and ecology of salmonid myxozoan parasites in the Lake Sammamish watershed, Washington. To address this knowledge gap, we tested water samples for Ceratonova shasta, Parvicapsula minibicornis and Tetracapsuloides bryosalmonae DNA from 84 sites distributed throughout the Lake Sammamish watershed in fall 2019 and 74 sites in spring 2020. Our surveillance identified zones with high waterborne parasite loads and provides a proof of concept for this approach that could be expanded throughout the larger Lake Washington watershed.

Light trap and larval trawl collection of bigheaded carp in the Missouri River Basin, 2016

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In the Lamine, Moreau, and Little Chariton rivers, light traps were used to capture larval bigheaded carps moving into tributaries. Traps were set on a weekly basis in each river, at roughly 1-kilometer intervals up to 7 km upstream. Ichthyoplankton tows were done in locations near the confluence of each tributary with the Missouri River, and within the Missouri River itself. Data includes identification of larval fish, size, and developmental information of subsampled invasive carp species. Flow readings were taken at up to five points across the channel with a Marsh-McBirney flow meter, and water quality was measured at the surface with a YSI ProPlus before retrieval of light traps.

Cytochrome oxidase subunit I (COI) and internally transcribed spacer region I (ITS1) DNA sequences of Tetracapsuloides bryosalmonae parasites in kidney tissue collected from various water bodies in Montana, USA from 2016 to 2019

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This dataset contains DNA sequences of two genomic regions, cytochrome oxidase subunit one and internally transcribed spacer region one, obtained from Tetracapsuloides bryosalmonae infecting the tissue of fish collected from various locations throughout western and central Montana, USA.

Data on the detection of Cumberlandia monodonta using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Big Piney River, Missouri 2020 – 2022 (ver. 2.0, August 2024)

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Data describe a designed environmental DNA (eDNA) survey for the detection of Spectaclecase (Cumberlandia monodonta, also referred to as Margaritifera monodonta) from field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater mussel species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect the presence of Spectaclecase DNA. Samples were collected from sites on the Big Piney River, Missouri from 2020 to 2022.

Data on the detection of Cumberlandia monodonta using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Big Piney River, Missouri 2020 – 2022 (ver. 2.0, August 2024)

공공데이터포털

Data describe a designed environmental DNA (eDNA) survey for the detection of Spectaclecase (Cumberlandia monodonta, also referred to as Margaritifera monodonta) from field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater mussel species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect the presence of Spectaclecase DNA. Samples were collected from sites on the Big Piney River, Missouri from 2020 to 2022.