This data documents the results of sampling for the white-nose syndrome fungus, Pseudogymnoascus destructans (Pd) at the USGS National Wildlife Health Center between 2013-2020. Data are reported on the county level. Locations are accurate to county only. We used data collected at winter locations only (hibernaculum) for this data set.

This data documents the results of sampling for the white-nose syndrome fungus, Pseudogymnoascus destructans (Pd) at the USGS National Wildlife Health Center between 2013-2020. Data are reported on the county level. Locations are accurate to county only. We used data collected at winter locations only (hibernaculum) for this data set.

This data documents the results of sampling for the white-nose syndrome fungus, Pseudogymnoascus destructans (Pd) at the USGS National Wildlife Health Center between 2007-2022. Data are reported on the county level. Locations are accurate to county only. We used data collected at winter locations only (hibernaculum) for this data set.

This data represents the number of positive and negative Pd (Pseudogymnoascus destructans) detections by county over the sampling period 2008-2012. Pd is the fungus that is the causative agent of white-nose syndrome.

This data represents the number of positive and negative Pd (Pseudogymnoascus destructans) detections by county over the sampling period 2008-2012. Pd is the fungus that is the causative agent of white-nose syndrome.

This dataset includes data used to summarize trends and identify best-fit models to explain patterns in presence-absence and abundance of Pseudogymnoascus destructans (Pd) in environmental substrates and on bats within six bat hibernacula at different stages of white-nose syndrome (WNS). Data relating to environmental substrates include: dates and relative spatial locations of samples collected within study hibernacula, presence and quantity of Pd in samples based on qPCR analysis, and daily temperature parameters at each sample location on the days samples were collected. Data relating to bats include: dates and relative spatial locations of hibernating bats that were sampled, species, sex, weight(g), forearm length(mm), body mass index (weight/forearm), proportion of the wing with visible fungus or fluorescence characteristic of WNS under hand-held UVA light and presence and quantity of Pd in wing-skin swab samples based on qPCR analysis. Measures of time since first detection of WNS at each study hibernaculum are also included in the dataset.

These data were collected as part of a voluntary initiative to create a White-Nose Syndrome Diagnostic Laboratory Network among laboratories participating in research and surveillance for Pseudogymonascus destructans (Pd) - the fungal pathogen causing White-Nose Syndrome in bats. Pd_qPCR_InterlaboratoryLODdata.xlsx is raw qPCR data from multiple laboratories running serial dilutions of Pd gBlock in known concentrations for the collectively used Muller (2013) Pd qPCR assay. Pd_qPCR_InterlaboratoryResults_LOD.xlsx contains the data output for each laboratory from running a generic LOD/LOQ calculator script. the generic LOD/LOQ calculator script is available at:https://github.com/cmerkes/qPCR_LOD_Calc. Pd_qPCR_InterlaboratoryPTResults_PanelData.xlsx contains the raw qPCR data from multiple laboratories running blinded samples spiked with known concentrations of Pd conidia. Each sample was extracted once and run in triplicate using the Muller (2013) assay. Pd_qPCR_InterlaboratoryPTResults_PanelResults contains the results of the blinded samples in each laboratory panel as both qPCR Ct values per replicate, and final overall sample result according to the WNS Case Definition. Pd_qPCR_InterlaboratoryPTResults_Standards.xlsx contains the results of the standard curves run by each laboratory in conjunction with the blinded sample panel.

These data were collected as part of a voluntary initiative to create a White-Nose Syndrome Diagnostic Laboratory Network among laboratories participating in research and surveillance for Pseudogymonascus destructans (Pd) - the fungal pathogen causing White-Nose Syndrome in bats. Pd_qPCR_InterlaboratoryLODdata.xlsx is raw qPCR data from multiple laboratories running serial dilutions of Pd gBlock in known concentrations for the collectively used Muller (2013) Pd qPCR assay. Pd_qPCR_InterlaboratoryResults_LOD.xlsx contains the data output for each laboratory from running a generic LOD/LOQ calculator script. the generic LOD/LOQ calculator script is available at:https://github.com/cmerkes/qPCR_LOD_Calc. Pd_qPCR_InterlaboratoryPTResults_PanelData.xlsx contains the raw qPCR data from multiple laboratories running blinded samples spiked with known concentrations of Pd conidia. Each sample was extracted once and run in triplicate using the Muller (2013) assay. Pd_qPCR_InterlaboratoryPTResults_PanelResults contains the results of the blinded samples in each laboratory panel as both qPCR Ct values per replicate, and final overall sample result according to the WNS Case Definition. Pd_qPCR_InterlaboratoryPTResults_Standards.xlsx contains the results of the standard curves run by each laboratory in conjunction with the blinded sample panel.

This work is part of an experimental trial investigating the effects of microclimate conditions of temperature and humidity on a fungal pathogen, Pseudogymnoascus destructans (Pd), that causes white-nose syndrome (WNS) disease in hibernating bats. As part of the trial, tri-colored bats (Perimyotis subflavus) were exposed to Pseudogymnoascus destructans (Pd) and allowed to hibernate in chambers with a variety of temperature and humidity conditions. Bats were euthanized after 83 days. A portion of the wing was rolled around dental wax dowels, fixed in 10% neutral buffered formalin, processed and stained with periodic acid-Schiff, and assessed by light microscopy for evidence of fungal infection. Three types of cutaneous infection were described histologically, including characteristic WNS cupping erosions, neutrophilic pustules with fungal hyphae, and fungal hyphae in the stratum corneum with dermal necrosis. Bats with any of these three conditions were scored as WNS-positive by histology. Only 11% (10/95) of bats scored as positive by histology. Of the 10 bats scored as positive, 3 bats had cupping erosions containing fungal hyphae and 7 bats had either neutrophilic pustules containing fungal hyphae, dermal necrosis associated with intra-epidermal fungal hyphae, or both. Overall, lack of infection and disease outcomes in this experiment limited our ability to make robust conclusions about the influence of microclimates on the development of WNS in bats.

This work is part of an experimental trial investigating the effects of microclimate conditions of temperature and humidity on a fungal pathogen, Pseudogymnoascus destructans (Pd), that causes white-nose syndrome (WNS) disease in hibernating bats. As part of the trial, tri-colored bats (Perimyotis subflavus) were exposed to Pseudogymnoascus destructans (Pd) and allowed to hibernate in chambers with a variety of temperature and humidity conditions. Bats were euthanized after 83 days. A portion of the wing was rolled around dental wax dowels, fixed in 10% neutral buffered formalin, processed and stained with periodic acid-Schiff, and assessed by light microscopy for evidence of fungal infection. Three types of cutaneous infection were described histologically, including characteristic WNS cupping erosions, neutrophilic pustules with fungal hyphae, and fungal hyphae in the stratum corneum with dermal necrosis. Bats with any of these three conditions were scored as WNS-positive by histology. Only 11% (10/95) of bats scored as positive by histology. Of the 10 bats scored as positive, 3 bats had cupping erosions containing fungal hyphae and 7 bats had either neutrophilic pustules containing fungal hyphae, dermal necrosis associated with intra-epidermal fungal hyphae, or both. Overall, lack of infection and disease outcomes in this experiment limited our ability to make robust conclusions about the influence of microclimates on the development of WNS in bats.