The Southeast Fisheries Science Center Mississippi Laboratories conducts standardized fisheries independent resource surveys in the Gulf of Mexico, South Atlantic, and U.S. Caribbean to provide abundance and distribution information to support regional and international stock assessments. The reef fish survey is conducted primarily on the outer continental shelf of the Gulf of Mexico along topographic features (e.g. reefs, banks and ledges) between Brownsville, TX to the Dry Tortugas, FL. A two-stage sampling design is used with the first stage or primary sampling units being blocks 10 minutes of latitude by 10 minutes of longitude and the second stage being randomly selected sites within the blocks. The first-stage units are selected by stratified random sampling, with stratum boundaries defined by geographic region (4 regions: South Florida, Northeast Gulf, Louisiana-Texas Shelf, and South Texas), and by reef habitat area (Blocks < 20 km² reef, Blocks > 20 km² reef). Sampling is conducted using a video camera array, vertical line gear and chevron traps with approximately 400 video cameras, 400 vertical line and 100 traps conducted. The camera array consists of four housings positioned orthogonally and center mounted at a height of 51 cm above the bottom of the array. Each housing contains a pair of black-and-white Videre stereo cameras along with a color mpeg camera. Sampling of reef sites with video cameras occurs only during daylight hours, with the first gear deployment one hour after sunrise and the last gear retrieval one hour prior to sunset. Video arrays soak for 35 minutes. At sites selected for fish sampling, a chevron (or arrow) fish trap or vertical line is used to capture fish for biological samples. The chevron fish trap is constructed with 1.5-inch vinyl-clad mesh. In its greatest dimensions, the trap is 1.76 m in length, 1.52 m in width and 0.61 m in depth. A 0.4 m by 0.29 m blow out panel is placed on one side and kept closed using 7-day magnesium releases. The fish trap soaks for one hour and is baited with squid. The vertical line consists of a mainline with 10 gangions. One 8/0, 11/0 or 15/0 circle hook is attached to each gangion and baited with mackerel (Scomber scombrus). The mainline is soaked for five minutes. Most of the animals captured are measured, weighed, tagged and then released. Those individuals which are moribund or have expired are retained to collect biological data pertaining to the life history of these fishes. Habitat mapping is conducted using the SIMRAD ME70 multibeam echosounder. At each site hydrological data is collected using Conductivity Temperature Depth sensor (CTD).

The Southeast Fisheries Science Center Mississippi Laboratories conducts standardized fisheries independent resource surveys in the Gulf of Mexico, South Atlantic, and U.S. Caribbean to provide abundance and distribution information to support regional and international stock assessments. Environmental profiles are acquired during all surveys and are averaged into one meter depth bins. The data are acquired with Sea-Bird SBE25 and SBE9 profilers equipped with water temperature, conductivity, dissolved oxygen, beam transmittance, and fluorescence sensors. The data are processed with Sea-Bird software using a standard processing protocol.

The Southeast Fisheries Science Center Mississippi Laboratories conducts standardized fisheries independent resource surveys in the Gulf of Mexico, South Atlantic, and U.S. Caribbean to provide abundance and distribution information to support regional and international stock assessments. In 2011, the R/V Gandy conducted a survey of reef fish located on the continental shelf and shelf-edge of the western Gulf of Mexico using bandit reels (vertical lines). Data were collected using using ten gangions, five #11/0 circle hooks and five #8/0 circle hooks. The data provided additional life history information for a red snapper benchmark stock assessment.

The Southeast Fisheries Science Center Mississippi Laboratories conducts standardized fisheries independent resource surveys in the Gulf of Mexico, South Atlantic, and U.S. Caribbean to provide abundance and distribution information to support regional and international stock assessments. Bottom longline gear was set at locations in Mexican and Cuban waters. The objective of the study was to assess the distribution and abundance of coastal and pelagic sharks. Ancillary objectives included collecting biological and environmental data and tagging.

The Commonwealth of the Northern Mariana Islands (CNMI), Division of Fish and Wildlife (DFW) staff conducted shore-based creel surveys which have 2 major sub-surveys; one to estimate participation (fishing effort), and one to provide catch-rate (CPUE), species composition data, and size of fishes. As is the case for all of these shore-based surveys, shore-based means fishing without a powered boat and can include effort such as spearfishing. DFW made early attempts at shore-based creel surveying back in the early years, but many problems existed and there were limited resources available. It is not likely that the older data was converted from the Apple to the PC environment, but this needs to be checked. A new survey design was created and implemented in about 2005 and is continuing. It has mostly focused on the west coast lagoon side of Saipan but recently has been extended to the south and part of the west coast where some shoreline areas are accessible as well. These data are considered confidential.

The Southeast Fisheries Science Center Mississippi Laboratories conducts standardized fisheries independent resource surveys in the Gulf of Mexico, South Atlantic, and U.S. Caribbean to provide abundance and distribution information to support regional and international stock assessments. Deepwater groupers, snappers and shrimp were sampled in the western Gulf of Mexico using a bottom trawl and bottom longline.

This fish and benthic composition database is the result of a multifaceted effort described below. The intent of this work is five fold: 1) To spatially characterize and monitor the distribution, abundance, and size of both reef fishes and macro-invertebrates (conch, lobster, Diadema); 2) To relate this information to in-situ data collected on associated benthic composition parameters; 3) To use this information to establish the knowledge base necessary for enacting management decisions in a spatial setting; 4) To establish the efficacy of those management decisions; and 5) To work with the National Coral Reef Monitoring Program to develop data collection standards and easily implemented methodologies for transference to other agencies and to work toward standardizing data collection throughout the US states and territories. Toward this end, the Center for Coastal Monitoring and Assessment's Biogeography Branch (BB) has been conducting research in Puerto Rico and the US Virgin Islands since 2000 and 2001, respectively. It is critical, with recent changes in management at both locations (e.g. implementation of MPAs) as well as proposed changes (e.g. zoning to manage multiple human uses) that action is taken now to accurately describe and characterize the fish/macro-invertebrate populations in these areas. It is also important that BB work closely with the individuals responsible for recommending and implementing these management strategies. Recognizing this, BB has been collaborating with partners at the University of Puerto Rico, National Park Service, US Geological Survey and the Virgin Islands Department of Planning and Natural Resources. To quantify patterns of spatial distribution and make meaningful interpretations, we must first have knowledge of the underlying variables determining species distribution. The basis for this work therefore, is the nearshore benthic habitats maps (less than 100 ft depth) created by NOAA's Biogeography Program in 2001 and NOS' bathymetry models. Using ArcView GIS software, the digitized habitat maps are stratified to select sampling stations. Sites are randomly selected within these strata to ensure coverage of the entire study region and not just a particular reef or seagrass area. At each site, fish, macro-invertebrates, and benthic composition information is then quantified following standardized protocols. By relating the data collected in the field back to the habitat maps and bathymetric models, BB is able to model and map species level and community level information. These protocols are standardized throughout the US Caribbean to enable quantification and comparison of reef fish abundance and distribution trends between locations. Armed with the knowledge of where "hot spots" of species richness and diversity are likely to occur in the seascape, the BB is in a unique position to answer questions about the efficacy of marine zoning strategies (e.g. placement of no fishing, anchoring, or snorkeling locations), and what locations are most suitable for establishing MPAs. Knowledge of the current status of fish/macro-invertebrate communities coupled with longer term monitoring will enable evaluation of management efficacy, thus it is essential to future management actions.

This fish and benthic composition database is the result of a multifaceted effort described below. The intent of this work is five fold: 1) To spatially characterize and monitor the distribution, abundance, and size of both reef fishes and macro-invertebrate (conch, lobster, Diadema); 2) To relate this information to in-situ data collected on associated benthic composition parameters; 3) To use this information to establish the knowledge base necessary for enacting management decisions in a spatial setting; 4) To establish the efficacy of those management decisions; and 5) To work with the National Coral Reef Monitoring Program to develop data collection standards and easily implemented methodologies for transference to other agencies and to work toward standardizing data collection throughout the US states and territories. Toward this end, the Center for Coastal Monitoring and Assessment's Biogeography Branch (BB) has been conducting research in Puerto Rico and the US Virgin Islands since 2000 and 2001, respectively. It is critical, with recent changes in management at both locations (e.g. implementation of MPAs) as well as proposed changes (e.g. zoning to manage multiple human uses) that action is taken now to accurately describe and characterize the fish/macro-invertebrate populations in these areas. It is also important that BB work closely with the individuals responsible for recommending and implementing these management strategies. Recognizing this, BB has been collaborating with partners at the University of Puerto Rico, National Park Service, US Geological Survey and the Virgin Islands Department of Planning and Natural Resources.To quantify patterns of spatial distribution and make meaningful interpretations, we must first have knowledge of the underlying variables determining species distribution. The basis for this work therefore, is the nearshore benthic habitats maps (less than 100 ft depth) created by NOAA's Biogeography Program in 2001 and NOS' bathymetry models. Using ArcView GIS software, the digitized habitat maps are stratified to select sampling stations. Sites are randomly selected within these strata to ensure coverage of the entire study region and not just a particular reef or seagrass area. At each site, fish, macro-invertebrates, and benthic composition information is then quantified following standardized protocols. By relating the data collected in the field back to the habitat maps and bathymetric models, BB is able to model and map species level and community level information. These protocols are standardized throughout the US Caribbean to enable quantification and comparison of reef fish abundance and distribution trends between locations. Armed with the knowledge of where "hot spots" of species richness and diversity are likely to occur in the seascape, the BB is in a unique position to answer questions about the efficacy of marine zoning strategies (e.g. placement of no fishing, anchoring, or snorkeling locations), and what locations are most suitable for establishing MPAs. Knowledge of the current status of fish/macro-invertebrate communities coupled with longer term monitoring will enable evaluation of management efficacy, thus it is essential to future management actions.

Fishery Independent Survey System (FINSS) is a national system that characterizes NMFS ocean observation activities, stock and ecosystem data collections during fishery-independent surveys conducted by NMFS Science Centers.

The Southeast Fisheries Science Center Mississippi Laboratories conducts standardized fisheries independent resource surveys in the Gulf of Mexico, South Atlantic, and U.S. Caribbean to provide abundance and distribution information to support regional and international stock assessments. In 2010, Congress appropriated supplemental funding to expand these surveys in the Gulf of Mexico in response to the Deepwater Horizon oil spill. Bottom longline stations were conducted throughout the northern Gulf of Mexico during 2011. Sampling occurred on multiple contracted fishing vessels simultaneously in the eastern, central and western Gulf of Mexico during all seasons. Data on species-specific catch rates, body lengths and sex were recorded.