The Southeast Fisheries Science Center Mississippi Laboratories conducts standardized fisheries independent resource surveys in the Gulf of Mexico, South Atlantic, and U.S. Caribbean to provide abundance and distribution information to support regional and international stock assessments. The reef fish survey is conducted primarily on the outer continental shelf of the Gulf of Mexico along topographic features (e.g. reefs, banks and ledges) between Brownsville, TX to the Dry Tortugas, FL. A two-stage sampling design is used with the first stage or primary sampling units being blocks 10 minutes of latitude by 10 minutes of longitude and the second stage being randomly selected sites within the blocks. The first-stage units are selected by stratified random sampling, with stratum boundaries defined by geographic region (4 regions: South Florida, Northeast Gulf, Louisiana-Texas Shelf, and South Texas), and by reef habitat area (Blocks < 20 km² reef, Blocks > 20 km² reef). Sampling is conducted using a video camera array, vertical line gear and chevron traps with approximately 400 video cameras, 400 vertical line and 100 traps conducted. The camera array consists of four housings positioned orthogonally and center mounted at a height of 51 cm above the bottom of the array. Each housing contains a pair of black-and-white Videre stereo cameras along with a color mpeg camera. Sampling of reef sites with video cameras occurs only during daylight hours, with the first gear deployment one hour after sunrise and the last gear retrieval one hour prior to sunset. Video arrays soak for 35 minutes. At sites selected for fish sampling, a chevron (or arrow) fish trap or vertical line is used to capture fish for biological samples. The chevron fish trap is constructed with 1.5-inch vinyl-clad mesh. In its greatest dimensions, the trap is 1.76 m in length, 1.52 m in width and 0.61 m in depth. A 0.4 m by 0.29 m blow out panel is placed on one side and kept closed using 7-day magnesium releases. The fish trap soaks for one hour and is baited with squid. The vertical line consists of a mainline with 10 gangions. One 8/0, 11/0 or 15/0 circle hook is attached to each gangion and baited with mackerel (Scomber scombrus). The mainline is soaked for five minutes. Most of the animals captured are measured, weighed, tagged and then released. Those individuals which are moribund or have expired are retained to collect biological data pertaining to the life history of these fishes. Habitat mapping is conducted using the SIMRAD ME70 multibeam echosounder. At each site hydrological data is collected using Conductivity Temperature Depth sensor (CTD).