Investigates the microbial communities of plants and Veggie pillow components with culturable and non-culturable methods providing qualitative and quantitative data. This studies also stresses the food safety component of fresh crops grown on the ISS. The VEG-03E technology demonstration was completed within a Veggie unit on the International Space Station (ISS). Microbiological characterization using molecular and culture-based methods was performed on leaves and roots from two harvests of green leaf lettuce, (Lactuca sativa cv. Waldmann’s) and associated rooting pillow components and Veggie chamber surfaces. Culture based enumeration and pathogen screening indicated the leafy greens were safe for consumption. Surface samples of the Veggie facility and plant pillows revealed low counts of bacteria and fungi and are commonly isolated on ISS. Community analysis was completed with 16S rRNA amplicon sequencing. Comparisons between pillow components, and plant tissue types from VEG-03D, E, and F revealed higher diversity in roots and rooting substrate than the leaves and wick. This work provides valuable information for food production-related research on the ISS and the impact of the plant microbiome on this unique closed environment.

Investigates the microbial communities of plants and Veggie pillow components with culturable and non-culturable methods providing qualitative and quantitative data. This studies also stresses the food safety component of fresh crops grown on the ISS. The VEG-03F technology demonstration took place within a Veggie unit on the International Space Station (ISS). Microbiological characterization using molecular methods was performed on leaves and roots from one harvest of red romaine lettuce (Lactuca sativa cv. ‘Outredgeous’) and associated rooting pillow components and Veggie chamber surfaces. Culture based enumeration and pathogen screening indicated the leafy greens were safe for consumption. Surface samples of the Veggie facility and plant pillows revealed low counts of bacteria and fungi and are commonly isolated on ISS. Community analysis was completed with 16S rRNA amplicon sequencing. Comparisons between pillow components, and plant tissue types from VEG-03D, E, and F revealed higher diversity in roots and rooting substrate than the leaves and wick. This work provides valuable information for food production-related research on the ISS and the impact of the plant microbiome on this unique closed environment.

Lettuce crops grown in the Veggie payload aboard ISS, harvested and returned for chemical, microbiological and molecular analysis

The ability to grow safe, fresh food to supplement packaged foods of astronauts in space has been an important goal for NASA. Food crops grown in space experience different environmental conditions than plants grown on Earth (e.g., reduced gravity, elevated radiation levels). To study the effects of space conditions, red romaine lettuce, Lactuca sativa cv ‘Outredgeous,’ plants were grown in Veggie plant growth chambers on the International Space Station (ISS) and compared with ground-grown plants. Multiple plantings were grown on ISS and harvested using either a single, final harvest, or sequential harvests in which several mature leaves were removed from the plants at weekly intervals. Ground controls were grown simultaneously with a 24–72 h delay using ISS environmental data. Food safety of the plants was determined by heterotrophic plate counts for bacteria and fungi, as well as isolate identification using samples taken from the leaves and roots. Molecular characterization was conducted using Next Generation Sequencing (NGS) to provide taxonomic composition and phylogenetic structure of the community. Leaves were also analyzed for elemental composition, as well as levels of phenolics, anthocyanins, and Oxygen Radical Absorbance Capacity (ORAC). Comparison of flight and ground tissues showed some differences in total counts for bacteria and yeast/molds (2.14 – 4.86 log10 CFU/g), while screening for select human pathogens yielded negative results. Bacterial and fungal isolate identification and community characterization indicated variation in the diversity of genera between leaf and root tissue with diversity being higher in root tissue, and included differences in the dominant genera. The only difference between ground and flight experiments was seen in the third experiment, VEG-03A, with significant differences in the genera from leaf tissue. Flight and ground tissue showed differences in Fe, K, Na, P, S, and Zn content and total phenolic levels, but no differences in anthocyanin and ORAC levels. This study indicated that leafy vegetable crops can produce safe, edible, fresh food to supplement to the astronauts’ diet, and provide baseline data for continual operation of the Veggie plant growth units on ISS.

The ability to grow safe, fresh food to supplement packaged foods of astronauts in space has been an important goal for NASA. Food crops grown in space experience different environmental conditions than plants grown on Earth (e.g., reduced gravity, elevated radiation levels). To study the effects of space conditions, red romaine lettuce, Lactuca sativa cv ‘Outredgeous,’ plants were grown in Veggie plant growth chambers on the International Space Station (ISS) and compared with ground-grown plants. Multiple plantings were grown on ISS and harvested using either a single, final harvest, or sequential harvests in which several mature leaves were removed from the plants at weekly intervals. Ground controls were grown simultaneously with a 24–72 h delay using ISS environmental data. Food safety of the plants was determined by heterotrophic plate counts for bacteria and fungi, as well as isolate identification using samples taken from the leaves and roots. Molecular characterization was conducted using Next Generation Sequencing (NGS) to provide taxonomic composition and phylogenetic structure of the community. Leaves were also analyzed for elemental composition, as well as levels of phenolics, anthocyanins, and Oxygen Radical Absorbance Capacity (ORAC). Comparison of flight and ground tissues showed some differences in total counts for bacteria and yeast/molds (2.14 – 4.86 log10 CFU/g), while screening for select human pathogens yielded negative results. Bacterial and fungal isolate identification and community characterization indicated variation in the diversity of genera between leaf and root tissue with diversity being higher in root tissue, and included differences in the dominant genera. The only difference between ground and flight experiments was seen in the third experiment, VEG-03A, with significant differences in the genera from leaf tissue. Flight and ground tissue showed differences in Fe, K, Na, P, S, and Zn content and total phenolic levels, but no differences in anthocyanin and ORAC levels. This study indicated that leafy vegetable crops can produce safe, edible, fresh food to supplement to the astronauts’ diet, and provide baseline data for continual operation of the Veggie plant growth units on ISS.

This dataset holds the Global Inventory Modeling and Mapping Studies-3rd Generation V1.2 (GIMMS-3G+) data for the Normalized Difference Vegetation Index (NDVI). NDVI was based on corrected and calibrated measurements from Advanced Very High Resolution Radiometer (AVHRR) data with a spatial resolution of 0.0833 degree and global coverage for 1982 to 2022. Maximum NDVI values are reported within twice monthly compositing periods (two values per month). The dataset was assembled from different AVHRR sensors and accounts for various deleterious effects, such as calibration loss, orbital drift, and volcanic eruptions. The data are provided in NetCDF format.

The Vegetation/Ecosystem Modeling and Analysis Project (VEMAP) is an ongoing multiinstitutional, international effort addressing the response of biogeography and biogeochemistry to environmental variability in climate and other drivers in both space and time domains. The objectives of VEMAP are the intercomparison of biogeochemistry models and vegetationtype distribution models (biogeography models) and determination of their sensitivity to changing climate, elevated atmospheric carbon dioxide concentrations, and other sources of altered forcing. The vegetation data set includes one variable: vegetation type. Vegetation types are defined physiognomically in terms of dominant lifeform and leaf characteristics (including leaf seasonal duration, shape, and size) and, in the case of grasslands, physiologically with respect to dominance of species with the C3 versus C4 photosynthetic pathway. The physiognomic classification criteria are based on our understanding of vegetation characteristics that influence biogeochemical dynamics (Running et al. 1994). The U.S. distribution of these types is based on a 0.5 degree latitude/longitude gridded map of Kuchler's (1964, 1975) potential natural vegetation provided by the TEM group (D. Kicklighter and A.D. McGuire, personal communication). Kuchler's map is based on current vegetation and historical information and, for purposes of VEMAP Phase I model experiments, is presumed to represent potential vegetation under current climate and atmospheric CO2 concentrations (355 ppm). A complete users guide to the VEMAP Phase I database which includes more information about this data set can be found at ftp://daac.ornl.gov/data/vemap-1/comp/Phase_1_User_Guide.pdf. ORNL DAAC maintains additional information associated with the VEMAP Project. Data Citation: This data set should be cited as follows: Kittel, T. G. F., N. A. Rosenbloom, T. H. Painter, D. S. Schimel, H. H. Fisher, A. Grimsdell, VEMAP Participants, C. Daly, and E. R. Hunt, Jr. 1998. VEMAP Phase I Database, revised. Available on-line from Oak Ridge National Laboratory Distributed Active Archive Center, Oak Ridge, Tennessee, U.S.A.