Data for "Estrogenic Activity of Perfluoro Carboxylic and Sulfonic Acids in Rainbow Trout Estrogen Receptor Binding and Liver Slice Vtg mRNA Expression Assays. Mark A. Tapper, Jeffrey S. Denny, Barbara R. Sheedy, Ben Johnson, and Richard C. Kolanczyk. Applied In Vitro Toxicology 2023 9:1, 13-22". This dataset is associated with the following publication: Tapper, M., J. Denny, B. Sheedy, B. Johnson, and R. Kolanczyk. Estrogenic Activity of Perfluoro Carboxylic and Sulfonic Acids in Rainbow Trout Estrogen Receptor Binding and Liver Slice Vtg mRNA Expression Assays. Applied In Vitro Toxicology. Mary Ann Liebert, Inc., Larchmont, NY, USA, 9(1): 13-22, (2023).

This dataset is from screening 94 diverse chemicals for estrogen receptor (ER) activation in a competitive rainbow trout ER binding assay and a trout liver slice vitellogenin mRNA expression assay. This dataset is associated with the following publication: Hornung, M., M. Tapper, J. Denny, B. Sheedy, R. Erickson, T. Sulerud, R. Kolanczyk, and P. Schmieder. Avoiding false positives and optimizing identification of true negatives in estrogen receptor binding and agonist/antagonist assays. Applied In vitro Toxicology. Mary Ann Liebert, Inc., Larchmont, NY, USA, 3(2): 163-181, (2017).

This dataset is from screening 94 diverse chemicals for estrogen receptor (ER) activation in a competitive rainbow trout ER binding assay and a trout liver slice vitellogenin mRNA expression assay. This dataset is associated with the following publication: Hornung, M., M. Tapper, J. Denny, B. Sheedy, R. Erickson, T. Sulerud, R. Kolanczyk, and P. Schmieder. Avoiding false positives and optimizing identification of true negatives in estrogen receptor binding and agonist/antagonist assays. Applied In vitro Toxicology. Mary Ann Liebert, Inc., Larchmont, NY, USA, 3(2): 163-181, (2017).

This ScienceHub entry was developed for the published paper: Consoer et al., 2016, Toxicokinetics of perfluorooctane sulfonate in rainow trout (Oncorhynchus mykiss), Environ. Toxicol. Chem. 35:717-727. Individual rainbow trout were exposed to PFOS by bolus injection (elimination studies) or by adding PFOS to incoming water (branchial uptake studies). The trout were fitted with indwelling catheters and urinary cannulae to permit periodic collection of blood and urine. Additional sampling was conducted to evaluate PFOS uptake from and elimination to respired water. Data obtained from each fish was evaluated using a clearance-volume pharmacokinetic model. Modeled kinetic parameters were then averaged to develop summary statistics which were used as a basis for interpreting modeled results and making comparisons to a previous study of rainbow trout exposed to perfluorooctanoate (PFOA; Consoer et al., 2014, Aquat. Toxicol. 156:65-73). The results of this study, combined with that of the previous PFOA study, suggest that PFOA is a substrate for renal transporters in fish while glomerular filtration alone may be sufficient to explain the observed renal elimination of PFOS. These findings demonstrate that models developed to predict the bioaccumulation of perfluoroalkyl acids by fish must account for differences in renal clearance of individual compounds. This dataset is associated with the following publication: Consoer, D., A. Hoffman , P. Fitzsimmons , P. Kosian , and J. Nichols. Toxicokinetics of perfluorooctane sulfonate in rainbow trout (Oncorhynchus mykiss). ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 35(3): 717-727, (2016).

The dataset includes data from six experiments in which adult male fathead minnows were exposed to five concentrations of a per- or polyfluorinated alkyl substance (PFAS) and a 17beta-estradiol positive control for 96 hours. Meta data on each fish exposed including treatment, replicate, sex, whole body wet weight, and gonad weights are included. Endpoints measured include nuptial tubercle scores, gonadosomatic index, and hepatic expression of four estrogen regulated mRNA transcripts: vitellogenin, estrogen receptor alpha, apolipoprotein eb, and insulin-like growth factor 1. The data set also includes water quality data collected over the course of each exposure, and measured concentrations of the test chemicals in water and plasma. The PFAS tested include: Perfluorooctanoic acid; 1H,1H,8H,8H-Perfluorooctane-1,8-diol; 1H,1H,10H,10H-Perfluorodecane-1,10-diol; 1H,1H,8H,8H-Perfluoro-3,6-dioxaoctane-1,8-diol; and Perfluoro-2-methyl-3-oxahexanoic acid. This dataset is associated with the following publication: Villeneuve, D., B. Blackwell, J. Cavallin, J. Collins, J. Hoang, R. Hofer, K. Houck, K. Jensen, M. Kahl, R. Kutsi, A. Opseth, K. Santana Rodriguez, C. Schaupp, E. Stacy, and G. Ankley. Verification of In Vivo Estrogenic Activity for Four Per- and Polyfluoroalkyl Substances (PFAS) Identified as Estrogen Receptor Agonists via New Approach Methodologies. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 57(9): 3794-3803, (2023).

Data set to support the manuscript "Increased endocrine activity of xenobiotic chemicals as mediated by metabolic activation". Contains analytical measurement of metabolites, binding potential to the estrogen receptor, and gene activation to vitellogenin induction in the rainbow trout assays. Chemicals of interest in this study were phenolphthalein, phenolphthalin, and 4,4’-methylenedianiline. This dataset is associated with the following publication: Kolanczyk, R., J. Denny, B. Sheedy, V. Olson, J. Serrano, and M. Tapper. Increased Endocrine Activity of Xenobiotic Chemicals as Mediated by Metabolic Activation.. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 42(12): 2747-2757, (2023).

Files contain summary reports of chemical and Mass Spectrometry raw and analyzed data used as basis for plot generation and text discussions in the Cyclic phenone metabolism manuscript. File dataset includes a Definition Xcel file listing the supporting files in set. Specifically, the dataset includes individual chemical slice exposure Xcel data summaries for the model cyclic phenones DPK, CBP and CPK as well as well as hepatocyte cytosol exposure data to the same chemicals. Each file contains data organized in labelled tabs that includes General Experimental information, Quantitative and qualitative methods used to process/analyze the raw data for parent and chemical metabolites, chemical mass balances and conclusions. Power point files with the summarized data were presented in the manuscript's Supplemental data. This dataset is associated with the following publication: Serrano, J., M. Tapper, R. Kolanczyk, B. Sheedy, T. Lahren, D. Hammermeister, J. Denny, M. Hornung, A. Kubatova, P. Kosian, J. Voelker, and P. Schmieder. Metabolism of cyclic phenones in rainbow trout in vitro assays. XENOBIOTICA. Taylor & Francis, Inc., Philadelphia, PA, USA, 50(2): 115-131, (2019).

Files contain summary reports of chemical and Mass Spectrometry raw and analyzed data used as basis for plot generation and text discussions in the Cyclic phenone metabolism manuscript. File dataset includes a Definition Xcel file listing the supporting files in set. Specifically, the dataset includes individual chemical slice exposure Xcel data summaries for the model cyclic phenones DPK, CBP and CPK as well as well as hepatocyte cytosol exposure data to the same chemicals. Each file contains data organized in labelled tabs that includes General Experimental information, Quantitative and qualitative methods used to process/analyze the raw data for parent and chemical metabolites, chemical mass balances and conclusions. Power point files with the summarized data were presented in the manuscript's Supplemental data. This dataset is associated with the following publication: Serrano, J., M. Tapper, R. Kolanczyk, B. Sheedy, T. Lahren, D. Hammermeister, J. Denny, M. Hornung, A. Kubatova, P. Kosian, J. Voelker, and P. Schmieder. Metabolism of cyclic phenones in rainbow trout in vitro assays. XENOBIOTICA. Taylor & Francis, Inc., Philadelphia, PA, USA, 50(2): 115-131, (2019).

Microarray datasets used in the analysis. This dataset is associated with the following publication: Rosen, M., K. Das, J. Rooney, B. Abbott, C. Lau, and C. Corton. PPARα-independent transcriptional targets of perfluoroalkyl acids revealed by transcript profiling. TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 387: 95-107, (2017).