Data includes quantification of microcystin by ELISA and quantification of gene copy number for Microcystis aeruginosa. In addition the genomic sequences associated with glucose addition to lake water are shown. This dataset is not publicly accessible because: public link too long. It can be accessed through the following means: https://gcc02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fsra%2FPRJNA786865&data=04%7C01%7CLinz.David%40epa.gov%7Cf41cc9e7fe7449eb4b1308d9bbc99a0f%7C88b378b367484867acf976aacbeca6a7%7C0%7C0%7C637747296829394696%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=F%2FUVwqnPj4pzWRiccZKHXh2jcxZ9bWxx1MD1Ux%2B1TBM%3D&reserved=0. Format: https://gcc02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fsra%2FPRJNA786865&data=04%7C01%7CLinz.David%40epa.gov%7Cf41cc9e7fe7449eb4b1308d9bbc99a0f%7C88b378b367484867acf976aacbeca6a7%7C0%7C0%7C637747296829394696%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=F%2FUVwqnPj4pzWRiccZKHXh2jcxZ9bWxx1MD1Ux%2B1TBM%3D&reserved=0. This dataset is associated with the following publication: Vesper, S., N. Sienkiewicz, I. Struewing, D. Linz, and J. Lu. Prophylactic Addition of Glucose Suppresses Cyanobacterial Abundance in Lake Water. Life. MDPI AG, Basel, SWITZERLAND, 12(3): 385, (2022).

Data shows that prophylactic addition of glucose to Harsha Lake water samples could inhibit cyanobacteria growth based on metagenomic sequencing data used to examine differences in the composition of bacterial communities between Treated and Control containers. The sequencing data shows that the addition of glucose to a container receiving weekly additions of Lake water suppressed the cyanobacterial populations during the entire summer bloom season. This dataset is associated with the following publication: Linz, D., I. Struewing, N. Sienkiewicz, A.D. Steinman, C.G. Partridge, K. McIntosh, J. Lu, and S. Vesper. Periodic Addition of Glucose Suppressed Cyanobacterial Abundance in Additive Lake Water Samples during the Entire Bloom Season. Journal of Water Resource and Protection. Scientific Research Publishing, Inc., Irvine, CA, USA, 16: 140-15, (2024).

(1) qPCR and RT-qPCR for cyanotoxin producing genes, and (2) some water quality parameters. This dataset is associated with the following publication: Duan, X., C. Zhang, I. Struewing, X. Li, H. Allen, and J. Lu. Cyanotoxin-encoding genes as powerful predictors of cyanotoxin production during harmful cyanobacterial blooms in an inland freshwater lake: Evaluating a novel early-warning system. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 830: 154568, (2022).

(1) qPCR and RT-qPCR for cyanotoxin producing genes, and (2) some water quality parameters. This dataset is associated with the following publication: Duan, X., C. Zhang, I. Struewing, X. Li, H. Allen, and J. Lu. Cyanotoxin-encoding genes as powerful predictors of cyanotoxin production during harmful cyanobacterial blooms in an inland freshwater lake: Evaluating a novel early-warning system. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 830: 154568, (2022).

Analysis of Microcystis aeruginosa physiology by spectral flow cytometry: Impact of chemical and light exposure. new technique to observe photosynthesis to monitor the health or cyanobacteria and their reaction to hydrogen peroxide. This dataset is not publicly accessible because: The public would require specialized software in order to view and interpret files. It can be accessed through the following means: Email zucker.robert@epa.gov. Format: The data in the paper is acquired in proprietary format from the manufacturer of the equipment. This data presented in the paper can be read only by using the software from the manufacturer which is quite costly and not feasible to purchase unless the scientist owns the equipment. This dataset is associated with the following publication: Brentjens, E., E. Beall, and R. Zucker. Analysis of Microcystis aeruginosa physiology by spectral flow cytometry: Impact of chemical and light exposure. PLOS Water. Public Library of Science, San Francisco, CA, USA, 2(10): e0000177, (2023).

With increasing concerns about freshwater cyanobacteria blooms, there is a need to identify which waterbodies are at risk for developing these blooms, especially those that produce cyanotoxins. To address this concern, we developed spatial statistical models using the US National Lakes Assessment, a survey with over 3,000 spring and summer observations of cyanobacteria abundance and microcystin concentration in lakes across the conterminous US. We combined these observations with other nationally available data to model which lake and watershed factors best explain the presence of harmful cyanobacterial blooms. We then used these models to estimate the cyanobacteria abundance and probability of microcystin detection in 124,500 lakes across the CONUS. This dataset includes the compiled data used to generate the models and the dataset used to generate prediction for a much larger population of lakes. The data package includes two tabular data files, two tabular metadata files, and one methods document.

Data from NLA 2012 was used to assess biovolume results for cyanobacteria (phytoplankton) in relation to both landscape and in lake factors. Citation information for this dataset can be found in the EDG's Metadata Reference Information section and Data.gov's References section.

Data from NLA 2012 was used to assess biovolume results for cyanobacteria (phytoplankton) in relation to both landscape and in lake factors. Citation information for this dataset can be found in the EDG's Metadata Reference Information section and Data.gov's References section.

The datasets include: 1. the measurements of glucose concentrations in each mesocosm, 2. the sonde measurements in each of the mesocosms at the beginning and end of the experiment, 3.the quantities of the cyanobacteria measured by FlowCam at each week of the experiment, 4. the microcystin concentrations in each mesocosm for the first 4 weeks of the experiment, 5. the concentration of total nitrogen and phosphorous in the mesocosms at the beginning and end of the experiment. This dataset is associated with the following publication: Gastaldo, C., and S. Vesper. HABS-BLOCKS© Inhibited Microcystis and Planktothrix and Reduced Microcystin Concentrations in a Lake Water Mesocosm Study. Microorganisms. MDPI, Basel, SWITZERLAND, 13(5): 1074, (2025).