Data includes quantification of microcystin by ELISA and quantification of gene copy number for Microcystis aeruginosa. In addition the genomic sequences associated with glucose addition to lake water are shown. This dataset is not publicly accessible because: public link too long. It can be accessed through the following means: https://gcc02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fsra%2FPRJNA786865&data=04%7C01%7CLinz.David%40epa.gov%7Cf41cc9e7fe7449eb4b1308d9bbc99a0f%7C88b378b367484867acf976aacbeca6a7%7C0%7C0%7C637747296829394696%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=F%2FUVwqnPj4pzWRiccZKHXh2jcxZ9bWxx1MD1Ux%2B1TBM%3D&reserved=0. Format: https://gcc02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fsra%2FPRJNA786865&data=04%7C01%7CLinz.David%40epa.gov%7Cf41cc9e7fe7449eb4b1308d9bbc99a0f%7C88b378b367484867acf976aacbeca6a7%7C0%7C0%7C637747296829394696%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=F%2FUVwqnPj4pzWRiccZKHXh2jcxZ9bWxx1MD1Ux%2B1TBM%3D&reserved=0. This dataset is associated with the following publication: Vesper, S., N. Sienkiewicz, I. Struewing, D. Linz, and J. Lu. Prophylactic Addition of Glucose Suppresses Cyanobacterial Abundance in Lake Water. Life. MDPI AG, Basel, SWITZERLAND, 12(3): 385, (2022).

Data includes quantification of microcystin by ELISA and quantification of gene copy number for Microcystis aeruginosa. In addition the genomic sequences associated with glucose addition to lake water are shown. This dataset is not publicly accessible because: public link too long. It can be accessed through the following means: https://gcc02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fsra%2FPRJNA786865&data=04%7C01%7CLinz.David%40epa.gov%7Cf41cc9e7fe7449eb4b1308d9bbc99a0f%7C88b378b367484867acf976aacbeca6a7%7C0%7C0%7C637747296829394696%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=F%2FUVwqnPj4pzWRiccZKHXh2jcxZ9bWxx1MD1Ux%2B1TBM%3D&reserved=0. Format: https://gcc02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fsra%2FPRJNA786865&data=04%7C01%7CLinz.David%40epa.gov%7Cf41cc9e7fe7449eb4b1308d9bbc99a0f%7C88b378b367484867acf976aacbeca6a7%7C0%7C0%7C637747296829394696%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=F%2FUVwqnPj4pzWRiccZKHXh2jcxZ9bWxx1MD1Ux%2B1TBM%3D&reserved=0. This dataset is associated with the following publication: Vesper, S., N. Sienkiewicz, I. Struewing, D. Linz, and J. Lu. Prophylactic Addition of Glucose Suppresses Cyanobacterial Abundance in Lake Water. Life. MDPI AG, Basel, SWITZERLAND, 12(3): 385, (2022).

The datasets include: 1. the measurements of glucose concentrations in each mesocosm, 2. the sonde measurements in each of the mesocosms at the beginning and end of the experiment, 3.the quantities of the cyanobacteria measured by FlowCam at each week of the experiment, 4. the microcystin concentrations in each mesocosm for the first 4 weeks of the experiment, 5. the concentration of total nitrogen and phosphorous in the mesocosms at the beginning and end of the experiment. This dataset is associated with the following publication: Gastaldo, C., and S. Vesper. HABS-BLOCKS© Inhibited Microcystis and Planktothrix and Reduced Microcystin Concentrations in a Lake Water Mesocosm Study. Microorganisms. MDPI, Basel, SWITZERLAND, 13(5): 1074, (2025).

Large lakes provide a variety of ecological services to surrounding cities and communities. Many of these services are supported by ecological processes that are threatened by the increasing prevalence of cyanobacterial blooms which occur as aquatic ecosystems experience cultural eutrophication. Over the past 10 years, Lake Erie experienced cyanobacterial blooms of increasing severity and frequency, which have resulted in impaired drinking water for the surrounding communities. Cyanobacterial blooms may impact ecological processes that support other services, but many of these impacts have not been documented. Secondary production (production of primary consumers) is an important process that supports economically important higher trophic levels. Cyanobacterial blooms may influence secondary production because 1) cyanobacteria are a poor quality food resource and 2) cyanotoxins may be harmful to consumers. Over three years at 36 sites across the western basin of Lake Erie, we measured 3 indices of secondary production: growth of a native unionid mussel, the size of young-of-year dreissenid mussels, and the mass of colonizing animals on a Hester-Dendy sampler. These indices were related to models with and without cyanobacterial data to assess whether cyanobacteria are associated with variation in secondary production in the western basin of Lake Erie. The results suggest cyanobacterial abundance alone is only weakly associated with secondary production, but that cyanotoxins have a bigger effect on secondary production. Given recent summer’s high cyanobacteria abundance, this impact on secondary production has the potential to undermine Lake Erie’s ability to support important ecosystem services.

Large lakes provide a variety of ecological services to surrounding cities and communities. Many of these services are supported by ecological processes that are threatened by the increasing prevalence of cyanobacterial blooms which occur as aquatic ecosystems experience cultural eutrophication. Over the past 10 years, Lake Erie experienced cyanobacterial blooms of increasing severity and frequency, which have resulted in impaired drinking water for the surrounding communities. Cyanobacterial blooms may impact ecological processes that support other services, but many of these impacts have not been documented. Secondary production (production of primary consumers) is an important process that supports economically important higher trophic levels. Cyanobacterial blooms may influence secondary production because 1) cyanobacteria are a poor quality food resource and 2) cyanotoxins may be harmful to consumers. Over three years at 36 sites across the western basin of Lake Erie, we measured 3 indices of secondary production: growth of a native unionid mussel, the size of young-of-year dreissenid mussels, and the mass of colonizing animals on a Hester-Dendy sampler. These indices were related to models with and without cyanobacterial data to assess whether cyanobacteria are associated with variation in secondary production in the western basin of Lake Erie. The results suggest cyanobacterial abundance alone is only weakly associated with secondary production, but that cyanotoxins have a bigger effect on secondary production. Given recent summer’s high cyanobacteria abundance, this impact on secondary production has the potential to undermine Lake Erie’s ability to support important ecosystem services.

Understanding the factors causing cyanobacterial harmful algal blooms (cHABs) through a better characterization of the cyanobacterial and associated aquatic microbiome community. Assessing the role of the microbial community in terms of nutrient cycling. Using genomic tools to better predict which blooms have the capacity to become toxic. All data are subjects of a publication containing method details, full QA/QC, interpretations and conclusions. Citation: Crevecoeur, S., Edge, T. A., Watson, L. C., Watson, S. B., Greer, C. W., Ciborowski, J. J. H., Diep, N., Dove, A., Drouillard, K. G., Frenken, T., McKay, R. M., Zastepa, A., & Comte, J. (2023). Spatio-temporal connectivity of the aquatic microbiome associated with cyanobacterial blooms along a Great Lake riverine-lacustrine continuum. Frontiers in microbiology, 14, 1073753. https://doi.org/10.3389/fmicb.2023.1073753; and Crevecoeur, S., Phillips, L., Zastepa, A., Comte, J., Diep, N., Dove, A., Edge, T., Frenken, T., McKay, R. M., & Waston, S. B. (2025). Spatio-Temporal Resolution of Microbial Functions and Taxa Associated With Cyanobacterial Harmful Algae Blooms Along a 500-Km Aquatic Continuum in the Lake Erie Watershed. Environmental microbiology, 27(10), e70183. https://doi.org/10.1111/1462-2920.70183 Genomic sequencing data is hosted online at the National Center for Biotechnology Information (NCBI) and is currently publicly available under Project Accession Number PRJNA877648 (https://www.ncbi.nlm.nih.gov/sra/PRJNA877648).

The data contained in the worksheet provides the quantity data of Cyanobacterial 16S sequences, qPCR and water quality parameters. This dataset is associated with the following publication: Li, H., T. Miller, J. Lu, and R. Goel. Nitrogen fixation contribution to nitrogen cycling during cyanobacterial blooms in Utah Lake. CHEMOSPHERE. Elsevier Science Ltd, New York, NY, USA, 302: 134784, (2022).

The data contained in the worksheet provides the quantity data of Cyanobacterial 16S sequences, qPCR and water quality parameters. This dataset is associated with the following publication: Li, H., T. Miller, J. Lu, and R. Goel. Nitrogen fixation contribution to nitrogen cycling during cyanobacterial blooms in Utah Lake. CHEMOSPHERE. Elsevier Science Ltd, New York, NY, USA, 302: 134784, (2022).

This data release contains cyanotoxin, chlorophyll-a, and pheophytin-a concentration, cyanobacterial genetics, phytoplankton community composition, and multiparameter sonde data collected from 20 sites in five northeastern United States river basins (Penobscot (ME), Santuit (MA), York (VA-WV), Salem (NJ), and Peconic (NY)). Solid Phase Adsorption Toxin Tracking (SPATT) passive samplers were deployed at all sites between August 31 and September 2, 2020, and retrieved after 7 days. Discrete water samples were collected when SPATTs were deployed, and at 2 sites (USGS station IDs 01670257, 0167014792), samples were also collected when the SPATTs were recovered. Sonde data were collected when deploying and retrieving the SPATTs. The SPATT samples were extracted and analyzed for cyanotoxins (anatoxin-a, cylindrospermopsin, microcystin, saxitoxin) by enzyme-linked immunosorbent assay (ELISA) and liquid chromatography tandem mass spectrometry (LC/MS/MS). Sonde data included measurements of water temperature, pH, specific conductance, dissolved oxygen, turbidity, chlorophyll, phycocyanin, and phycoerythrin fluorescence (in relative fluorescence units and micrograms per liter), and nitrate, however some sensors were not available at all locations. Discrete samples were analyzed for total cyanotoxin concentration (anatoxin-a, cylindrospermopsin, microcystin, saxitoxin) by ELISA and LC/MS/MS, chlorophyll-a, pheophytin-a, cyanotoxin synthetase genes, and phytoplankton community composition. Saxitoxin could not being analyzed by LC/MS/MS so an additional ELISA analysis was performed instead.

This data release contains cyanotoxin, chlorophyll-a, and pheophytin-a concentration, cyanobacterial genetics, phytoplankton community composition, and multiparameter sonde data collected from 20 sites in five northeastern United States river basins (Penobscot (ME), Santuit (MA), York (VA-WV), Salem (NJ), and Peconic (NY)). Solid Phase Adsorption Toxin Tracking (SPATT) passive samplers were deployed at all sites between August 31 and September 2, 2020, and retrieved after 7 days. Discrete water samples were collected when SPATTs were deployed, and at 2 sites (USGS station IDs 01670257, 0167014792), samples were also collected when the SPATTs were recovered. Sonde data were collected when deploying and retrieving the SPATTs. The SPATT samples were extracted and analyzed for cyanotoxins (anatoxin-a, cylindrospermopsin, microcystin, saxitoxin) by enzyme-linked immunosorbent assay (ELISA) and liquid chromatography tandem mass spectrometry (LC/MS/MS). Sonde data included measurements of water temperature, pH, specific conductance, dissolved oxygen, turbidity, chlorophyll, phycocyanin, and phycoerythrin fluorescence (in relative fluorescence units and micrograms per liter), and nitrate, however some sensors were not available at all locations. Discrete samples were analyzed for total cyanotoxin concentration (anatoxin-a, cylindrospermopsin, microcystin, saxitoxin) by ELISA and LC/MS/MS, chlorophyll-a, pheophytin-a, cyanotoxin synthetase genes, and phytoplankton community composition. Saxitoxin could not being analyzed by LC/MS/MS so an additional ELISA analysis was performed instead.