Data describe results of a set of laboratory experiments using a previously described quantitative thiaminase I activity assay to test the thiaminase activity of whole zebrafish homogenates. Parameters described include the thiaminase activity data, dilution factors, incubation time, and tissue weight assayed. Experiments were conducted at the USGS Columbia Environmental Research Center, Columbia, MO in 2006.

Data describe results of a set of laboratory experiments using a previously described quantitative thiaminase I activity assay to test the thiaminase activity of whole zebrafish homogenates. Parameters described include the thiaminase activity data, dilution factors, incubation time, and tissue weight assayed. Experiments were conducted at the USGS Columbia Environmental Research Center, Columbia, MO in 2006.

Among the data required by the U.S. Food and Drug Administration (FDA) for approval of an aquaculture drug are the data that characterize the depletion of a drugs marker residue from the edible fillet tissue of fish after exposure. Eugenol is the marker residue for AQUI-S 20E, a product proposed for use as a sedative for fish.Rainbow trout (Onchorynchus mykiss; a representative cold water fish species) was exposed to AQUI-S 20E in water at a temperature of 9 C, a temperature that is representative of the lower range of temperatures where rainbow trout would be sedated. Eighty fish were exposed to a nominal AQUI-S 20E concentration of 100 mg/L for 60 min. Groups of 16 fish were sampled immediately after 60 min of exposure (the 0 min sample group), then at 15, 30, 90, and 150 min after the fish were transferred to fresh, flowing water. Skin-on fillets from each fish were analyzed for eugenol concentrations by using a FDA approved method for determining eugenol concentrations in fish fillet tissue. The method involved extracting eugenol from the tissue with acetonitrile, recovering eugenol from the tissue extract by using solid phase extraction techniques, and determining eugenol concentrations in the final extract by using high pressure liquid chromatography with absorbance detection techniques.Results indicated that maximum eugenol concentrations in the fillet tissue were measured immediately after the exposure (mean, 47.5 g/g). Eugenol concentrations decreased to 1.9 g/g by 150 min after the fish were transferred to fresh, flowing water. The depletion of eugenol from the fillet tissue was rapid (t1/2 = 31.7 min).

Among the data required by the U.S. Food and Drug Administration (FDA) for approval of an aquaculture drug are the data that characterize the depletion of a drugs marker residue from the edible fillet tissue of fish after exposure. Eugenol is the marker residue for AQUI-S 20E, a product proposed for use as a sedative for fish.Rainbow trout (Onchorynchus mykiss; a representative cold water fish species) was exposed to AQUI-S 20E in water at a temperature of 9 C, a temperature that is representative of the lower range of temperatures where rainbow trout would be sedated. Eighty fish were exposed to a nominal AQUI-S 20E concentration of 100 mg/L for 60 min. Groups of 16 fish were sampled immediately after 60 min of exposure (the 0 min sample group), then at 15, 30, 90, and 150 min after the fish were transferred to fresh, flowing water. Skin-on fillets from each fish were analyzed for eugenol concentrations by using a FDA approved method for determining eugenol concentrations in fish fillet tissue. The method involved extracting eugenol from the tissue with acetonitrile, recovering eugenol from the tissue extract by using solid phase extraction techniques, and determining eugenol concentrations in the final extract by using high pressure liquid chromatography with absorbance detection techniques.Results indicated that maximum eugenol concentrations in the fillet tissue were measured immediately after the exposure (mean, 47.5 g/g). Eugenol concentrations decreased to 1.9 g/g by 150 min after the fish were transferred to fresh, flowing water. The depletion of eugenol from the fillet tissue was rapid (t1/2 = 31.7 min).

The data set provides all data that were reported in results, tables, and figures associated with Stinckens et al., "The effect of thyroperoxidase and deiodinase inhibition on anterior swim bladder inflation in the zebrafish". The data set includes: 1) Effects of three test chemicals on thyroperoxidase enzyme activity 2) Effects of three test chemicals on deiodinase enzyme activity 3) Thyroid hormone (T3 and T4) concentrations measured in exposed larvae 4) Measures of posterior and anterior swimbladder chamber inflation and surface areas. 5) Data on swimming behavior (distance) of exposed fish. 6) Measured concentrations of the test chemicals in the test media and tissues. This dataset is associated with the following publication: Stinckens, E., L. Vergauwen, B. Blackwell, G. Ankley, D. Villeneuve, and D. Knapen. Effect of thyroperoxidase and deiodinase inhibition on anterior swim bladder inflation in the zebrafish. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 54(10): 6213-6223, (2020).

The data set provides all data that were reported in results, tables, and figures associated with Stinckens et al., "The effect of thyroperoxidase and deiodinase inhibition on anterior swim bladder inflation in the zebrafish". The data set includes: 1) Effects of three test chemicals on thyroperoxidase enzyme activity 2) Effects of three test chemicals on deiodinase enzyme activity 3) Thyroid hormone (T3 and T4) concentrations measured in exposed larvae 4) Measures of posterior and anterior swimbladder chamber inflation and surface areas. 5) Data on swimming behavior (distance) of exposed fish. 6) Measured concentrations of the test chemicals in the test media and tissues. This dataset is associated with the following publication: Stinckens, E., L. Vergauwen, B. Blackwell, G. Ankley, D. Villeneuve, and D. Knapen. Effect of thyroperoxidase and deiodinase inhibition on anterior swim bladder inflation in the zebrafish. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 54(10): 6213-6223, (2020).

Alternatives to chemicals for controlling dreissenid mussels are desirable for environmental compatibility, but few alternatives exist. Previous studies have evaluated the use of electrified fields for stunning and/or killing planktonic life stages of dreissenid mussels, however, the available literature on the use of electrified fields to control adult dreissenid mussels is limited. We evaluated the effects of sinusoidal alternating current (AC) and square- wave pulse direct current (PDC) exposure on the survival of zebra mussels at water temperatures of 10, 15, and 22°C. Peak voltage gradients of ~ 17 and 30 Vp/cm in the AC and PDC exposures, respectively, were continuously applied for 24, 48, or 72 h. Peak power densities ranged from 77,999 to 107,199 μW/cm3 in the AC exposures and 245,320 to 313,945 μW/cm3 in the PDC exposures. The peak dose ranged from 6,739 to 27,298 Joules/cm3 and 21,306 to 80,941 Joules/cm3 in the AC and PDC exposures, respectively. Mortality ranged from 2.7 to 92.7% in the AC treated groups and from 24.0 to 98.7% in PDC treated groups. Mortality increased with corresponding increases in water temperature and exposure duration, and we observed more zebra mussel mortality in the PDC exposures.

The data consists of the responses (survival, growth, and/or reproduction) of test organisms were determined in six concentrations of toxicants in 7-day toxicity tests or in four different feeding rates in 7-day feeding experiments. Specifically we evaluated the sensitivity of 2 mussel species (Villosa constricta and Lampsilis siliquoidea) and P. promelas and C. dubia using effluents in 7-d exposures. We then refined the method by determining the best feeding rate of algal mixture for 1-, 2-, and 3-wk-old L. siliquoidea in a 7-d feeding experiment, and using derived optimal feeding rates to assess the sensitivity of the 3 ages of juveniles in a 7-d NaCl test. Finally, we conducted an interlaboratory study among 13 laboratories to evaluate the performance of a 7-d NaCl test with L. siliquoidea.

Effects of developmental 17beta-estradiol exposure on microbiota and behavior in zebrafish. This dataset is associated with the following publication: Catron, T., A. Swank, L. Wehmas, D. Phelps, S. Keely, N. Brinkman, J. McCord, R. Singh, J. Sobus, C. Wood, M. Strynar, E. Wheaton, and T. Tal. Microbiota alter metabolism and mediate neurodevelopmental toxicity of 17β-estradiol. Scientific Reports. Nature Publishing Group, London, UK, 9: Article number 7064, (2019).

Effects of developmental 17beta-estradiol exposure on microbiota and behavior in zebrafish. This dataset is associated with the following publication: Catron, T., A. Swank, L. Wehmas, D. Phelps, S. Keely, N. Brinkman, J. McCord, R. Singh, J. Sobus, C. Wood, M. Strynar, E. Wheaton, and T. Tal. Microbiota alter metabolism and mediate neurodevelopmental toxicity of 17β-estradiol. Scientific Reports. Nature Publishing Group, London, UK, 9: Article number 7064, (2019).