Concentrations of somatic coliphage, F+ coliphage, E. coli and enterococci. This dataset is associated with the following publication: Korajkic, A., B. McMinn, and V.J. Harwood. The Effect of Protozoa Indigenous to Lakewater and Wastewater on Decay of Fecal Indicator Bacteria and Coliphage. PATHOGENS. MDPI, Basel, SWITZERLAND, 12(3): 378, (2023).

Dataset for male-specific and somatic coliphage densities in raw sewage and ambient waters. Citation information for this dataset can be found in the EDG's Metadata Reference Information section and Data.gov's References section.

The fasta files (Genome_Set01.zip) contain the reference-assisted de novo assemblies (as contigs) of three Escherichia coli isolates. The table contains rows as isolates (yellow) and columns as attributes (green) for each individual genome. This dataset is associated with the following publication: Gomez-Alvarez, V., and J. Hoelle-Schwalbach. Draft Genome Sequences of Antibiotic-Resistant Escherichia coli Isolates from U.S. Wastewater Treatment Plants. Microbiology Resource Announcements. American Society for Microbiology, Washington, DC, USA, 8(23): e00351-19, (2019).

Human-associated genetic marker concentrations in raw sewage. This dataset is associated with the following publication: Schoen, M.E., A.B. Boehm, J. Soller, and O. Shanks. Contamination Scenario Matters when Using Viral and Bacterial Human-Associated Genetic Markers as Indicators of a Health Risk in Untreated Sewage-Impacted Recreational Waters. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 54(20): 13101–13109, (2020).

This dataset includes concentrations of culture-based fecal indicator concentrations, microbial source tracking marker concentrations, results from Bayesian statistical analysis. This dataset is associated with the following publication: McMinn, B., A. Korajkic, J. Kelleher, A. Diedrich, A. Pemberton, J. Willis, M. Sivaganesan, B. Shireman, A. Doyle, and O. Shanks. Quantitative fecal pollution assessment with bacterial, viral, and molecular methods in small stream tributaries. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 951: 175740, (2024).

Coliphage are alternative fecal indicators that may be suitable surrogates for viral pathogens, but a majority of standard detection methods utilize insufficient sample volumes (1-100 mL) for routine detection in environmental waters. Here we compare three somatic and F+ coliphage enumeration methods based on a paired measurement from 1L samples collected from the Great Lakes region (n=74). Methods include: 1) a dead-end hollow fiber ultrafilter combined with single agar layer plaque assay (D-HFUF-SAL); 2) a modified SAL (M-SAL); and 3) a direct membrane filtration (DMF) technique. Overall, D-HFUF-SAL outperformed all other methods as it yielded the lowest frequency of non-detects [(ND); 10.8%] and the highest average coliphage concentrations (2.51 ± 1.02 log10 plaque forming unit/liter (PFU/L) and 0.79 ± 0.71 log10 PFU/L for somatic and F+, respectively). M-SAL yielded 29.7% ND and average concentrations of 2.26 ± 1.15 log10 PFU/L (somatic) and 0.59 ± 0.82 log10 PFU/L (F+). DMF performed worse compared to D-HFUF-SAL and M-SAL methods (ND of 65.6%; average somatic coliphage concentration 1.52 ± 1.32 log10 PFU/L, with no F+ detected), indicating this procedure is unsuitable for 1L surface water sample volumes. This study represents an important step toward the use of a coliphage method for recreational water quality criteria purposes. This dataset is associated with the following publication: McMinn, B., E. Rhodes, E. Huff, P. Wanjugi, M. Ware, S. Nappier, M. Cyterski, O. Shanks, K. Oshima, and A. Korajkic. Comparison of somatic and F+ coliphage enumeration methods with large volume surface water samples. JOURNAL OF VIROLOGICAL METHODS. Elsevier Science Ltd, New York, NY, USA, 261: 63-66, (2018).

Data contains information about bacterial concentrations in the water column in a mixed use watershed in Northeast GA. This dataset is associated with the following publication: Sowah, R., K. Bradshaw, B. Snyder, D. Spidle, and M. Molina. Evaluation of the soil and water assessment tool (SWAT) for simulating E. coli concentrations at the watershed-scale. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 746: 140669, (2020).

The dataset includes Escherichia coli concentrations collected by Isleta Pueblo in the Middle Rio Grande in Albuquerque, NM, from 2017 to 2020. These data were pulled from National Water Quality Monitoring Council, 2021, Water Quality Portal, accessed December 5, 2021, at https://www.waterqualitydata.us.

The dataset includes Escherichia coli concentrations collected by Isleta Pueblo in the Middle Rio Grande in Albuquerque, NM, from 2017 to 2020. These data were pulled from National Water Quality Monitoring Council, 2021, Water Quality Portal, accessed December 5, 2021, at https://www.waterqualitydata.us.

Data set includes measurements used in Figure 2.