This dataset includes concentrations of culture-based fecal indicator concentrations, microbial source tracking marker concentrations, results from Bayesian statistical analysis. This dataset is associated with the following publication: McMinn, B., A. Korajkic, J. Kelleher, A. Diedrich, A. Pemberton, J. Willis, M. Sivaganesan, B. Shireman, A. Doyle, and O. Shanks. Quantitative fecal pollution assessment with bacterial, viral, and molecular methods in small stream tributaries. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 951: 175740, (2024).

Decomposition data of bacterial and viral fecal indicators in common human pollution types. This dataset is associated with the following publication: Wanjugi, P., M. Sivaganesan, A. Korajkic, C. Kelty, B. McMinn, R. Ulrich, V. Harwood, and O. Shanks. Differential Decomposition of Bacterial and Viral Fecal Indicators in Common Human Pollution Types. WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 105: 591-601, (2016).

Concentrations of viral/bacterial indicators and viral pathogens in wastewater. This dataset is associated with the following publication: Korajkic, A., J. Kelleher, O. Shanks, M. Herrmann, and B. McMinn. Effectiveness of two wastewater disinfection strategies for the removal of fecal indicator bacteria, bacteriophage, and enteric viral pathogens concentrated using dead-end hollow fiber ultrafiltration (D-HFUF). SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 831: 154861, (2022).

Dataset for male-specific and somatic coliphage densities in raw sewage and ambient waters. Citation information for this dataset can be found in the EDG's Metadata Reference Information section and Data.gov's References section.

Somatic coliphages are alternative indicators of fecal pollution and are attractive surrogate for viral pathogens. Here we report the draft genome sequences of three replicate plaques from a novel myoviridae bacteriophage isolated from raw wastewater. Genomes were similar to felix01virus phage and are predicted to contain up to 159 protein coding genes. This dataset is associated with the following publication: Keely, S., M. Herrmann, A. Korajkic, N. Brinkman, B. McMinn, S. Fout, and E. Villegas. Genome Sequences of Escherichia Bacteriophages Isolated from Raw Wastewater. Microbiology Resource Announcements. American Society for Microbiology, Washington, DC, USA, 8(26): e00135-19, (2019).

Water quality measurements at 3 beach sites in the Great Lakes from the summer of 2015: Edgewater Beach, OH; Grant Park, WI; Washington Park, MI. Data include associated environmental parameters (meteorological and hydrologic). Microbial data include cultured fecal indicator bacteria, coliphage, and molecular marker (qPCR) measurements. Detailed description of data collection activities can be found in Wanjugi et al. 2018: https://www.sciencedirect.com/science/article/pii/S004313541830349X. Portions of this dataset are inaccessible because: It is included. They can be accessed through the following means: ScienceHub. Format: Excel File with a "MetaData" tab describing the data columns. This dataset is associated with the following publication: Cyterski, M., O. Shanks, P. Wanjugi, B. McMinn, A. Korajkic, K. Oshima, and R. Haugland. Bacterial and Viral Fecal Indicator Predictive Modeling Using Gradient Boosting at Three Great Lakes Recreational Beach Sites. WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 223: 118970, (2022).

,Alternative irrigation waters (rivers, ponds, and reclaimed water) can harbor bacterial foodborne pathogens like Salmonella enterica and Listeria monocytogenes, potentially contaminating fruit and vegetable commodities. Detecting foodborne pathogens using qPCR-based methods may accelerate testing methods and procedures compared to culture-based methods. This study compared detection of S. enterica and L. monocytogenes by qPCR (real-time PCR) and culture methods in irrigation waters to determine the influence of water type (river, pond, and reclaimed water), season (winter, spring, summer, and fall), or volume (0.1, 1, and 10 L) on sensitivity, accuracy, specificity, and positive (PPV), and negative (NPV) predictive values of these methods. Water samples were collected by filtration through modified Moore swabs (MMS) over a 2-year period at 11 sites in the Mid-Atlantic U.S. on a bi-weekly or monthly schedule. For qPCR, bacterial DNA from culture-enriched samples (n = 1,990) was analyzed by multiplex qPCR specific for S. enterica and L. monocytogenes. For culture detection, enriched samples were selectively enriched, isolated, and PCR confirmed. PPVs for qPCR detection of S. enterica and L. monocytogenes were 68% and 67%, respectively. The NPV were 87% (S. enterica) and 85% (L. monocytogenes). Higher levels of qPCR/culture agreement were observed in spring and summer compared to fall and winter for S. enterica; for L. monocytogenes, lower levels of agreement were observed in winter compared to spring, summer, and fall. Reclaimed and pond water supported higher levels of qPCR/culture agreement compared to river water for both S. enterica and L. monocytogenes, indicating that water type may influence the agreement of these results.,

Human-associated genetic marker concentrations in raw sewage. This dataset is associated with the following publication: Schoen, M.E., A.B. Boehm, J. Soller, and O. Shanks. Contamination Scenario Matters when Using Viral and Bacterial Human-Associated Genetic Markers as Indicators of a Health Risk in Untreated Sewage-Impacted Recreational Waters. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 54(20): 13101–13109, (2020).

Dataset describes performance of a culture-based method to concentrate and quantify somatic and F+ coliphages. This dataset is associated with the following publication: McMinn, B., E. Huff, E. Rhodes, and A. Korajkic. Concentration and Quantification of Somatic and F+ Coliphage from Recreational Waters. JOURNAL OF VIROLOGICAL METHODS. Elsevier Science Ltd, New York, NY, USA, 249: 58-65, (2017).