Oxidative stress (OS) is a contributing factor to the neuro, cardiac, and pulmonary effects caused by adverse metabolic states, such as obesity and type II diabetes, as well as inhalation of air borne toxicants, such as ozone (O3). The objective of this study is to understand diet/O3 interactions on OS parameters in young male Brown Norway rats maintained on regular (Purina 5001), high fructose (FRUC, TD.89247), or high fat (FAT, TD.06414) diet for 16 wks followed by exposure to either filtered air or 0.8 ppm O3 under an acute (1 d for 5 h) or subacute (5 h/d, 1 d/wk for four wks) paradigm. After 18 h of the last exposure, measures of ROS production (NAD(P)H:quinone oxidoreductase (NQO1) and NADH-Ubiquinone reductase (UBIQ-RD) levels), antioxidant homeostasis (total antioxidant substance (TAS) and γ-Glutamylcysteine synthetase (gGCS) activity), and oxidative damage (total aconitase activity and protein carbonyl (PC) content) were assayed in selected brain regions. Diet/O3 interaction did not have a global effect in the brain, but did show limited regional and OS parameter specific effects. HIP showed a significant interaction between FRUC diet/O3. Aconitase in CER showed a significant interaction between diet and O3. However, regional effects of either O3 or diet alone were more profound. Within the acute condition, there was a decrease in NQ01 and UBIQ-RD in STR and HIP, respectively, regardless of exposure. Also, CER and STR showed a change in TAS due to diet alone, while FC seemed to have a larger amount of TAS due to O3 alone. Diet appeared to affect gGCS negatively in all diet groups of HIP, and only in the FAT diet of STR. The CER also appear to have a decrease in PC in FRUC group and a general decrease in PC in all diets due to O3, while aconitase increased only in FRUC air exposed animals and control O3 exposed animals. Under the subacute condition, there was an increase of NQO1 activity in only the CER due to diets alone, while UBIQ-RD increased in only the FRUC group in FC and in both diet groups in HIP. TAS was decreased in FC only in the FAT group and a clear O3 effect where FAT increased the TAS and FRUC decreased in TAS. A significant interaction between diet/O3 was found in FC. The STR also showed a decrease in TAS in response to O3. Diet also increased PC formation within CER only in the FAT group, while HIP showed a decrease in PC after O3 exposure in controls. Aconitase in CER was affected both by diet in the filtered air group while O3 caused a decrease in controls and FRUC groups. O3 affected all groups within HIP and HYP. STR was most affected by FAT diet in both air and O3 groups. Diet seemed to be the driving factor in most OS measures. Overall, OS parameters measured do not suggest a consistent O3/diet interaction on oxidative damage pathways, but do give insight as to how high caloric diets could affect neuronal OS. This dataset is associated with the following publication: Valdez, J., A. Johnstone, J. Richards, J. Schmid, J. Royland, and P. Kodavanti. Interaction of Diet and Ozone Exposure on Oxidative Stress Parameters within Specific Brain Regions of Male Brown Norway Rats. International Journal of Molecular Sciences. MDPI AG, Basel, SWITZERLAND, 1-17, (2018).

Dataset for a study examining the cardiovascular effects from acute ozone exposure in rats fed with fish oil or olive oil diets. This dataset is associated with the following publication: Tong, H., S. Snow, M.C. Schladweiler, G. Carswell, B. Chorley, and U. Kodavanti. Fish Oil and Olive Oil-Enriched Diets Alleviate Acute Ozone-induced Cardiovascular Effects in Rats. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 409: 115296, (2020).

These are the data for the figures in the manuscript. This dataset is associated with the following publication: Corteselli, E., A. Gold, J. Surratt, T. Cui, P. Bromberg, L. Dailey, and J. Samet. Supplementation with Omega-3 Fatty Acids Potentiates Oxidative Stress in Human Airway Epithelial Cells Exposed to Ozone. ENVIRONMENTAL RESEARCH. Elsevier B.V., Amsterdam, NETHERLANDS, 187: 109627, (2020).

These are the data for the figures in the manuscript. This dataset is associated with the following publication: Corteselli, E., A. Gold, J. Surratt, T. Cui, P. Bromberg, L. Dailey, and J. Samet. Supplementation with Omega-3 Fatty Acids Potentiates Oxidative Stress in Human Airway Epithelial Cells Exposed to Ozone. ENVIRONMENTAL RESEARCH. Elsevier B.V., Amsterdam, NETHERLANDS, 187: 109627, (2020).

This data set was used for the manuscript titled "Ozone-induced Changes in Oxidative Stress Parameters in Brain regions of Adult, Middle-age, and Senescent Brown Norway Rats". It includes various biochemical endpoints and neurobehavior. This dataset is associated with the following publication: Kodavanti, P.R., M. Valdez, J. Richards, D. Agina-Obu, P. Phillips, K. Jarema, and U. Kodavanti. Ozone-induced changes in oxidative stress parameters in brain regions of adult, middle-age, and senescent Brown Norway rats. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 410: 115351, (2020).

This data set was used for the manuscript titled "Ozone-induced Changes in Oxidative Stress Parameters in Brain regions of Adult, Middle-age, and Senescent Brown Norway Rats". It includes various biochemical endpoints and neurobehavior. This dataset is associated with the following publication: Kodavanti, P.R., M. Valdez, J. Richards, D. Agina-Obu, P. Phillips, K. Jarema, and U. Kodavanti. Ozone-induced changes in oxidative stress parameters in brain regions of adult, middle-age, and senescent Brown Norway rats. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 410: 115351, (2020).

In this study, using male Wistar and Wistar-derived Goto-Kakizaki (GK) rats, which exhibit a non-obese type-2 diabetes phenotype, we investigated whether two key metabolic stressors, type-2 diabetes and a high-cholesterol atherogenic diet, exacerbate ozone-induced metabolic effects. Rats were fed a normal control diet (ND) or high-cholesterol diet (HCD) for 12 weeks starting at 4 week-age and then exposed to filtered air or 1.0-ppm ozone (6h/day) for 1 or 2 days. Metabolic responses were analyzed at the end of each day and after an 18-hour recovery period following the 2-day exposure. This dataset is associated with the following publication: Snow, S., A. Henriquez, A. Fisher, B. Vallanat, J. House, M.C. Schladweiler, C. Wood, and U. Kodavanti. Peripheral Metabolic Effects of Ozone Exposure in Healthy and Diabetic Rats on Normal or High-Cholesterol Diet. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 415(115427): 1, (2021).

In this study, we investigated effects of inhaled ozone exposure and high-cholesterol diet (HCD) in healthy Wistar and Wistar-derived Goto-Kakizaki (GK) rats, a non-obese model of type 2 diabetes. Male rats (4-week old) were fed normal diet or HCD for 12 weeks and then exposed to filtered air or 1.0 ppm ozone (6hrs/day) for 1 or 2 days. We examined pulmonary, vascular, hematology, and inflammatory responses after each exposure plus an 18-hr recovery period. This dataset is associated with the following publication: Snow, S., A. Henriquez, L. Thompson, C. Fisher, M.C. Schladweiler, C. Wood, and U. Kodavanti. Pulmonary and Vascular Effects of Acute Ozone Exposure in Diabetic Rats Fed an Atherogenic Diet. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 415(115430): 1, (2021).

In this study, we investigated effects of inhaled ozone exposure and high-cholesterol diet (HCD) in healthy Wistar and Wistar-derived Goto-Kakizaki (GK) rats, a non-obese model of type 2 diabetes. Male rats (4-week old) were fed normal diet or HCD for 12 weeks and then exposed to filtered air or 1.0 ppm ozone (6hrs/day) for 1 or 2 days. We examined pulmonary, vascular, hematology, and inflammatory responses after each exposure plus an 18-hr recovery period. This dataset is associated with the following publication: Snow, S., A. Henriquez, L. Thompson, C. Fisher, M.C. Schladweiler, C. Wood, and U. Kodavanti. Pulmonary and Vascular Effects of Acute Ozone Exposure in Diabetic Rats Fed an Atherogenic Diet. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 415(115430): 1, (2021).

Data include: trans-epithelial electrical resistance, FITC-dextran permeability, cell viability and gene expression (RNA and protein). This dataset is associated with the following publication: Faber, S., N. McNabb, P. Ariel, E. Aungst, and S. McCullough. Exposure Effects Beyond the Epithelial Barrier: Trans-Epithelial Induction of Oxidative Stress by Diesel Exhaust Particulates in Lung Fibroblasts in an Organotypic Human Airway Model. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 177(1): 140-155, (2020).