Data were collected from two laboratory rearing experiments conducted in 2018 of Trichoplusia ni caterpillars that had been parasitized by Copidosoma floridanum parasitoids. In the first experiment, parasitized caterpillars were fed artificial diets spiked with increasing concentrations of the phytochemical xanthotoxin in order to assess the effect of xanthotoxin on parasitoid success. In the second experiment, parasitized caterpillars were switched between diets with differing xanthotoxin concentration, such that all caterpillars experienced the same mean xanthotoxin concentration over their lives, but different treatments experienced different variability in xanthotoxin concentration. Data were recorded on caterpillar survival and parasitoid fitness components (e.g. number of offspring, proportion of offspring emerging from host, sex ratio of adult offspring).

,Life Table Data: Field-based, partial life table data for immature stages of Bemisia tabaci on cotton in Maricopa, Arizona, USA. Data were generated on approximately 200 individual insects per cohort with 2-5 cohorts per year for a total of 44 cohorts between 1997 and 2010. Data provide the marginal, stage-specific rates of mortality for eggs, and 1st, 2nd, 3rd, and 4th instar nymphs. Mortality is characterized as caused by inviability (eggs only), dislodgement, predation, parasitism and unknown. Detailed methods can be found in Naranjo and Ellsworth 2005 (Entomologia Experimentalis et Applicata 116(2): 93-108). The method takes advantage of the sessile nature of immature stages of this insect. Briefly, an observer follows individual eggs or settled first instar nymphs from natural populations on the underside of cotton leaves in the field with a hand lens and determines causes of death for each individual over time. Approximately 200 individual eggs and nymphs are observed for each cohort. Separately, densities of eggs and nymphs are monitored with standard methods (Naranjo and Flint 1994, Environmental Entomology 23: 254-266; Naranjo and Flint 1995, Environmental Entomology 24: 261-270) on a weekly basis.,Matrix Model Data: Life table data were used to provide parameters for population matrix models. Matrix models contain information about stage-specific rates for development, survival and reproduction. The model can be used to estimate overall population growth rate and can also be analyzed to determine which life stages contribute the most to changes in growth rates.,,

,We evaluated the flight behavior of E. giganteana in response to semiochemicals identified from other closely related Eucosma species, including: (Z)- and (E)-8-dodecenyl acetate, (E)-9-dodecenyl acetate, (Z)-8-dodecenol, (E,E)-8,10-dodecadienyl acetate, and (Z,E)-9,12-tetradecadienyl acetate. The goals of this study were to evaluate whether any of these compounds could improve capture of E. giganteana on clear sticky cards in the field, and whether the most attractive volatiles might affect flight behavior on a computer-automated flight mill assay. We found that there was significant attraction to (E)-8-dodecenyl acetate in two years in the field, which may possibly be a component in the pheromone blend for E. giganteana. On flight mills, E. giganteana flew an average of 23 km in a 24 h period. The presence of attractive stimuli (e.g., (E)-8-dodecenyl acetate) had arresting properties and decreasing flight distance on the mill by 78 to 80%. The longest flight distances were registered in the morning (4:00–12:00) and were 1.8-fold greater than flight distances and durations at night (20:00–4:00). (E)-8-dodecenyl acetate may be useful in behaviorally based monitoring and management strategies for E. giganteana. Overall, our research expands the knowledge on the chemical ecology of adult E. giganteana.,Dataset includes testing field-captured E. giganteana adults collected from UV light traps deployed at The Land Institute in Salina, KS, and hand-collected at night during the period of peak activity for E. giganteana. Two trials are included: a field baiting assay, and laboratory flight mill assay. In the field baiting assay, each field had three transects spaced at least 10 m apart, each with a full set of semiochemical treatments represented. Each trap within the transect was spaced 10 m apart. Each trap consisted of a 1.27-cm diameter PVC pipe hammered in row with the silflower to a finished height of 1 m, in line with the canopy of Silphium integrifolium. A single 30.4 cm × 30.4 cm clear sticky card (Alpha Scents, Canby, OR, USA) was folded in half and inserted in a 271 cm long sticky card ring holder (Olson Products Inc., Medina, OH, USA). The ring holder was bent at a 90° angle to wedge the card holder upright in position, which was subsequently wedged in the opening at the top of the PVC pipe. A single, capped LDPE 3-mL dropping bottle with one of the semiochemical treatments above was inserted in the top of the PCV pipe opening and affixed in place by tying it to the card holder with garden wire. Every week, the lures were replaced with a freshly prepared treatment and the position of the lure was rotated in the transect every two weeks. Traps were rotated because of the short duration of the flying season and resulted in every treatment occupying every position at least once. Sticky cards were changed on a weekly basis after the first recorded capture of an E. giganteana adult. Traps were deployed 7 June 2019 to 14 August 2019 and 15 June 2020 to 10 August 2020. In total, there were n = 3 replicates of each semiochemical treatment per field site. The number of E. giganteana and Lepidopteran nontargets was counted on each sticky card after freezing cards at −20 °C for at least 24 h.,For the flight mill assay, six adults were run simultaneously on the six flight mills described in the associated article (15-FMASM SDP Unit, Crist Instrument Co., Hagerstown, MD, USA) to test flight capacity. Each trial was started between 15:00 and 18:00 by gently blowing on the insect to initiate flight and run for 24 h in parallel. Assays were conducted at 21.4 ± 0.01 °C temperature and 54.2 ± 0.2% RH and monitored with a datalogger (UX100-011, Hobo, temp/RH logger, Onset, Bourne, MA, USA). The semiochemical treatments in the flight mill assay included (E)-8-dodecenyl acetate, (Z)-9-dodecenyl acetate, and an unbaited control (acetone solvent only). Semiochemicals were freshly prepared in LDPE dropping bottles, as in the field-baiting

,Trapping in 2023 with a linear set of dosages of (E)-8-dodecenyl acetate,Field trapping was done according to the methodology in Ruiz et al. 2022. The fields were located in North-Central Kansas at the Land Institute near Salina, KS. No pesticides were applied to these fields during the experiment in 2023. Starting the first week of June, six transects were set out, two in each Silphium integrifolium field. Each transect contained seven 30.4 cm x 30.4 cm sticky card traps (Alpha Scents, Canby, OR, USA) affixed to the top of a 1.27 cm diameter, three foot in length PVC pole that was hammered into the ground until sturdy. The cards were affixed using a 271 cm long sticky card ring holder (Olson Products Inc., Medina, OH, USA) that was bent to a 90° angle and placed inside the PVC pipe. Two large binder clips were also used to anchor the sticky card to its card holder.,The sticky traps in each transect were spaced 10 meters apart around the perimeter of the field. Within each transect, traps were baited with a linear increase in concentrations in 2023, including either a control (50 µl of acetone), a low concentration (50 µl of a solution made by mixing 5.75 µl of (E)-8-dodecenyl acetate in 5 ml of acetone), or a doubled concentration (11.5 µl of (E)-8-dodecenyl acetate diluted in 5 ml of acetone) of (E)-8-dodecenyl acetate (Alfa Chemistry, Ronkonkoma, NY, USA). All lures were added to a 3-ml LDPE dropping bottle (Wheaton, DWK Life Sciences, Millville, NJ, USA). The clear sticky card traps were collected and replaced biweekly until the first E. giganteana adult was caught, then traps were changed weekly. The lures and control bottles were replaced once every two weeks (with lure emissions confirmed out to 14 d in Ruiz et al. 2022) and their position in the field rotated at each change. Each lure was in each position twice over the course of the season.,When collected, the sticky cards were held in a 7.6 L (=2 gal) labeled Ziploc© bag transported back to USDA-ARS. All collected sticky traps were placed in a freezer for approximately 24 h. The total number of E. giganteana per trap and their distance from the lure in millimeters was recorded. In addition, the number of nontarget lepidoptera was recorded on each trap. Individual E. giganteana and non-target lepidoptera were only counted if more than half of the specimen was remaining on the sticky trap at the time of counting to ensure positive identification.,Trapping in 2024 with an exponential set of concentrations of (E)-8-dodecenyl acetate,Field trapping in 2024 was conducted similarly to that in 2023 with the following modifications. Three different fields located at the Land Institute were used (Table 1). [HS1] Pesticides were applied once to one of the fields and adjacent to one of the others. Three transects were deployed in each of the three fields. Each transect contained four traps for a total of 36 traps. The traps were assembled similarly to those used in 2023, but a hand-made sticky card was used instead of a manufactured one to improve captures. These sticky cards were made of a laminated 21.6 × 27.9 cm (=8.5 by 11 in) piece of white cardstock paper (Astrobright, Neenah, WI, USA) coated on both sides with TADⓇ all-weather adhesive (Trécé Adhesives Division, Adair, OK, USA). The sticky sides were covered with wax paper for ease of travel. Additionally, the sticky cards had a chicken wire cage placed over them in the field to try to prevent the capture of birds and other nontargets on the traps. Traps in 2024 were baited with an exponential set of concentrations of (E)-8-dodecenyl acetate. In each transect, there was a solvent only control (50 µl of acetone), a low concentration equivalent to the 2023 treatment (50 µl of a solution made of 5.75 µl of (E)-8-dodecenyl acetate diluted in 5 ml of acetone), a medium concentration (50 µl of a solution made of 78.5 µl of (E)-8-dodecenyl acetate diluted in 5 ml of acetone), and a high concentration (50 µl of a solution made

,Four to six-week-old larvae of Trogoderma variabile and Trogoderma inclusum were used for the experiment. Both strains were originally obtained from the field in north-central Kansas in 2016 and 2012, respectively. Colonies of these species were reared under controlled conditions in an environmental chamber set to a temperature of 27.5 °C, 65% RH, and 14:10 (L:D) h photoperiod. Both species were fed 300 g of ground dog food (SmartBlend, Lamb flavor, PurinaOne, St. Louis, MO, USA) with oats sprinkled on top and a moistened, crumpled paper towel placed on the surface in a 950-ml mason jar.,Treatments The long-lasting insecticide-incorporated polyethylene netting (2 × 2 mm mesh, D-Terrance, Vestergaard Inc., Lausanne, Switzerland) included 0.4% deltamethrin, or control netting that was identical in physical properties but without insecticide. These were used with the movement assay. We assessed the movement in the vicinity of important pheromonal and food kairomones after exposure to LLIN or control netting. Food consisted of 0.01 g of organic, unbleached flour (Heartland Mills, Marienthal, KS, USA), and pheromonal stimuli included a broad spectrum, multi-species lure (PTL lure, IL-108-10, Batch#1288200321, Insects Limited, Westfield, IN, USA), including Trogoderma spp pheromone (Ranabhat et al. 2023a). In each replicate, we used a single pellet (white color), and affixed it in place so it did not move in a Petri dish using a 1 × 1 mm square of parafilm. For each replication of testing, we used a fresh lure.,Movement Assay The movement of larvae after exposure to the 0.4 % deltamethrin LLIN or a control netting in response to food cues (using 0.01 g of flour) or with conspecific sex pheromones (using a single bead from a disaggregated PTL lure held in place with a small square of parafilm), was tracked in six individual arenas (100 × 15 mm D: H) with a piece of filter paper (85 mm D, Ahlstrom-Munksjö, Helsinki, Finland) lining the bottom for 30 min using a network camera (GigE, Basler AG, Ahrenburg, Germany) affixed 76 cm above and centered over the dishes. The Petri dishes were backlit using a LED light box (42 × 30 cm W:L LPB3, Litup, Shenzhen, China) to increase contrast and affixed in place with white foam board. The video was streamed to a computer and processed in Ethovision (v.14.5 Noldus Inc., Leesburg, VA, USA). Prior to use in the movement assay, larvae of T. variabile or T. inclusum were exposed to the 0.4% deltamethrin LLIN or a control netting for 1 min in a 21 × 21 cm square Petri dish, then their movement was tracked individually after a post-exposure holding duration of 1 min or 24 h. A small 1.1 cm hidden stimulus zone encircled each stimulus, midway and centered on each half of the arena wherein movement was tracked separately from each half of the arena (control vs. treatment). The total distance moved (cm), instantaneous velocity (cm/s), frequency of entering each half of the petri dish and stimulus zone, cumulative duration spent in each zone (s), and latency of entering each zone (s) over a 30 min trial period was logged after exposure to a given treatment. The control side of the arena remained empty. A total of n = 16 replicates were run per treatment combination for both species,No-Choice Release-Recapture Assay A release- recapture experiment was conducted for the larvae of both T. variabile and T. inclusum where larvae were exposed to the 0.4% deltamethrin LLIN and control netting for 1 min. After exposure, treated insects were released at one corner of the sanded plastic bin (60 × 41.6 × 16.5 cm L:W:H ). A commercial pitfall trap (Dome Trap™, Trécé, Inc., Adair, OK, USA) that contained a PTL lure (used only white beads as above), or 0.01 g flour, or no stimuli (unbaited for control), was deployed in the opposite corner, diagonally across from the release point in the bin. The bins were located in a large (4.8 × 2.1 × 6 m, L:W:H) walk-in environmental chamber (Percival Instruments, Dallas County, IA,

,Insects,Beetles used in this study were obtained from stock colonies maintained at the USDA Agricultural Research Service’s (ARS) Center for Grain and Animal Health Research (CGAHR) in Manhattan, KS, USA. Colonies of R. dominica and S. oryzae were reared on organic whole wheat kernels that had been tempered to 15% grain moisture. To subculture, a total of 50 adult individuals were placed on 200 mL of grain in a mason jar (capacity: 473 mL) and given 14 d to mate and lay eggs. At the end of that period, adult hosts were removed by sieving with a #10 sieve (2.00 mm; W.S Tyler Inc., Mentor, Ohio), and colonies were allowed to age for 3-weeks prior to using beetles as hosts for parasitoid rearing. Theocolax elegans were maintained separately on two different hosts, either R. dominica or S. oryzae for at least three full generations. Freshly emerged, healthy T. elegans were used for the experiments below. All colonies of parasitoids were maintained in a separate environmental chamber than host-only colonies to prevent cross-contamination. Colonies were maintained in mason jars and stored in an environmental chamber under constant conditions (27.5°C, 60% RH, 14:10 L:D).,Interactions with Predators,Laboratory studies were performed in 2022 and 2023 at the USDA Center for Grain and Animal Health Research (Manhattan, KS, USA). From July–October of each year, predators were collected weekly from local post-harvest food facilities, including the Kansas State Agronomy Farm (GPS: 39.2062227, -96.5951959), where S. oryzae and other stored product pests are abundantly found (Morrison et al. 2025[1] ). Most predators used in trials were collected by sweep netting (Bioquip Products, Inc., Rancho Dominguez, CA) sampling vegetation adjacent to grain bins or by hand collection and held temporarily in 1-gal (=3.98 L) Ziplocks, then immediately brought back to the lab in a cooler on insulated ice packs. In the lab, insects were processed by individually placing predators into a 950-mL mason jar with 10 S. oryzae from colonies. The predators were identified to family (Marshall 2006, Paquin et al. 2017). Mason jars with predators and S. oryzae were then placed on shelves in an environmental chamber set to constant conditions (27.5°C, 60% RH, 14:10 L:D). After 24 h, the jars were checked, and the number of S. oryzae consumed was recorded as well as the presence of any self-aggregation behavior of S. oryzae together and away from the predator, which was taken to be evidence for non-consumptive effects in the presence of the predator. The results of predators were only included when there were n = 3 or greater number of replicates.,Ethovision,Video-tracking coupled with Ethovision software v.14.0 (Noldus, Inc., Leesburg, VA: Noldus et al. 2002) was used to investigate the impact of natural enemy kairomones on the mobility and orientation of R. dominica and S. oryzae over short distances. This system has previously been used for analyzing the mobility and foraging behaviors of stored product insects (Wilkins et al. 2020; Ponce et al. 2022). Six arenas consisting of Petri dishes (VWR Petri dishes, 100 × 15 mm) with an 85-mm filter paper (Grade 1, Whatman, GE Healthcare, Chicago, IL) adhered to the bottom using double-sided sticky tape were arranged 80 cm below a network video camera (GigE, Basler AG, Ahrensburg, Germany). The movement of individual insects within each arena was simultaneously recorded on an adjacent computer. Four zones were monitored in Ethovision, including the two halves of the Petri dish (i.e. treatment half vs control half) and two 1 cm diameter zones nested in the middle of each half where stimuli were applied (treatment stimulus zone and control stimulus zone). The position of treatments was randomized between replicates and a total of n = 12 replicate assays were conducted for each treatment. For each assay, a single insect was introduced into the center of an arena and its movement was tracked for a total of 10 min. Several

Arthropods are important prey for most forest birds in Hawaii. The relative abundance of arthropods on koa (Acacia koa) changed significantly during an outbreak of the koa moth (Scotorythra paludicola) that occurred across much of Hawaii Island during 2013-2014. The outbreak resulted in large tracts of koa forest becoming defoliated by large numbers of koa moth caterpillars. This data release includes metadata and tabular data that documents how bird diets changed at Hakalau Forest National Wildlife Refuge during the outbreak. The data set documents numbers of koa moth caterpillars and other arthropod prey consumed by forest birds prior to, and during the koa moth outbreak. Diets were reconstructed by identifying arthropod body parts from bird fecal samples collected during mist-netting operations conducted at two study sites dominated by koa.

Arthropods are important prey for most forest birds in Hawaii. The relative abundance of arthropods on koa (Acacia koa) changed significantly during an outbreak of the koa moth (Scotorythra paludicola) that occurred across much of Hawaii Island during 2013-2014. The outbreak resulted in large tracts of koa forest becoming defoliated by large numbers of koa moth caterpillars. This data release includes metadata and tabular data that documents how bird diets changed at Hakalau Forest National Wildlife Refuge during the outbreak. The data set documents numbers of koa moth caterpillars and other arthropod prey consumed by forest birds prior to, and during the koa moth outbreak. Diets were reconstructed by identifying arthropod body parts from bird fecal samples collected during mist-netting operations conducted at two study sites dominated by koa.

,Data files for manuscript titled "Importance of color for artificial clay caterpillars as sentinel prey in maize, soybean, and prairie".,Metadata is contained within excel file that describes all variables for each tab.,Abstract from paper: The use of artificial clay caterpillars to measure predation pressure under real field conditions is one method that has garnered recent support for quantifying ecosystem services that beneficial insects provide. Here, we focus on color and ask whether it is an important variable that should be considered in studies using clay caterpillars as sentinel prey. We deployed a total of 1920 brown, cream, green, gray, terracotta, and white clay caterpillars onto maize, soybean, and prairie plants to test if lighter colored caterpillars will be attacked and retrieved more than caterpillars with darker colors. As hypothesized, color was a significant predictor with green and terracotta caterpillars performing best, whereas brown and gray caterpillars performed the worst. Interestingly, clay caterpillars were also attacked proportionally to the number of insects in the surrounding habitat. Combined, we suggest artificial clay caterpillars could be useful for rapid ecosystem function assessments, but only when their color is considered.,,