EPA data for Lane et al 2020 Water

공공데이터포털

QPCR standard curve data and example test sample data. This dataset is associated with the following publication: Lane, M.J., J.N. McNair, R.R. Rediske, S. Briggs, M. Sivaganesan, and R. Haugland. Simplified Analysis of Measurement Data from A Rapid E. coli qPCR Method (EPA Draft Method C) Using A Standardized Excel Workbook. WATER. MDPI AG, Basel, SWITZERLAND, 12(3): 775, (2020).

EPAdata MLS paper2

공공데이터포털

EPA Draft Method C QPCR cycle threshold (Ct) measurements of standardized reference materials and blinded test samples as described in D-EMMD-MEB-025-QAPP-01 and Journal article. This dataset is associated with the following publication: Aw, T., M. Sivaganesan, S. Briggs, E. Dreelin, A. Aslan, S. Dorevitch, A. Shrestha, N. Isaacs, J. Kinzelman, G. Kleinheinz, R. Noble, R. Rediske, B. Scull, S. Rosenberg, B. Weberman, T. Sivy, B. Southwell, S. Siefring, K. Oshima, and R. Haugland. Evaluation of multiple laboratory performance and variability in analysis of recreational freshwaters by a rapid Escherichia coli qPCR method (Draft Method C). WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 156: 465-474, (2019).

EPAdata MLS paper2

공공데이터포털

EPA Draft Method C QPCR cycle threshold (Ct) measurements of standardized reference materials and blinded test samples as described in D-EMMD-MEB-025-QAPP-01 and Journal article. This dataset is associated with the following publication: Aw, T., M. Sivaganesan, S. Briggs, E. Dreelin, A. Aslan, S. Dorevitch, A. Shrestha, N. Isaacs, J. Kinzelman, G. Kleinheinz, R. Noble, R. Rediske, B. Scull, S. Rosenberg, B. Weberman, T. Sivy, B. Southwell, S. Siefring, K. Oshima, and R. Haugland. Evaluation of multiple laboratory performance and variability in analysis of recreational freshwaters by a rapid Escherichia coli qPCR method (Draft Method C). WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 156: 465-474, (2019).

EPAdata MLS paper1

공공데이터포털

EPA Draft Method C QPCR cycle threshold (Ct) measurements of standardized reference materials as described in D-EMMD-MEB-025-QAPP-01 and Journal article. This dataset is associated with the following publication: Sivaganesan, M., T. Aw, S. Briggs, E. Dreelin, A. Aslan, S. Dorevitch, A. Shrestha, N. Isaacs, J. Kinzelman, G. Kleinheinz, R. Noble, R. Rediske, B. Scull, S. Rosenberg, B. Weberman, T. Sivy, B. Southwell, S. Siefring, K. Oshima, and R. Haugland. Standardized data quality acceptance criteria for a rapid Escherichia coli qPCR method (Draft Method C) for water quality monitoring at recreational beaches. WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 156: 456-464, (2019).

EPAdata MLS paper1

공공데이터포털

EPA Draft Method C QPCR cycle threshold (Ct) measurements of standardized reference materials as described in D-EMMD-MEB-025-QAPP-01 and Journal article. This dataset is associated with the following publication: Sivaganesan, M., T. Aw, S. Briggs, E. Dreelin, A. Aslan, S. Dorevitch, A. Shrestha, N. Isaacs, J. Kinzelman, G. Kleinheinz, R. Noble, R. Rediske, B. Scull, S. Rosenberg, B. Weberman, T. Sivy, B. Southwell, S. Siefring, K. Oshima, and R. Haugland. Standardized data quality acceptance criteria for a rapid Escherichia coli qPCR method (Draft Method C) for water quality monitoring at recreational beaches. WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 156: 456-464, (2019).

Dataset for development and evaluation of a qPCR method modification that mitigates DNA recovery losses for analyses of E. coli fecal indicator bacteria in Great Lakes region recreational waters

공공데이터포털

Results previously reported by EPA/ORD on the modified method have led to its adoption into a Standard Operating Procedure for labs performing qPCR analyses for E. coli at recreational beaches in the state of Michigan. This dataset provides further documentation of the performance and applicability of the modified method for recreational beach water testing including data from an extensive survey that can be used to determine when and where unacceptable levels of DNA losses may occur with and without this method modification in the Great Lakes region. Please see the attached dataset description document for additional information.

Cao et al 20xx Data Set (Version 1)

공공데이터포털

HF183/BacR287 qPCR data from standard curves and coastal water samples used to seed simulations in study. This dataset is associated with the following publication: Cao, Y., M. Sivaganesan, C. Kelty, D. Wang, A. Boehm, J. Griffith, S. Weisberg, and O. Shanks. A Human Fecal Contamination Score for Ranking Recreational Sites using the HF183/BacR287 Quantitative Real-Time PCR Method. WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 128: 148-156, (2018).

Cao et al 20xx Data Set (Version 1)

공공데이터포털

HF183/BacR287 qPCR data from standard curves and coastal water samples used to seed simulations in study. This dataset is associated with the following publication: Cao, Y., M. Sivaganesan, C. Kelty, D. Wang, A. Boehm, J. Griffith, S. Weisberg, and O. Shanks. A Human Fecal Contamination Score for Ranking Recreational Sites using the HF183/BacR287 Quantitative Real-Time PCR Method. WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 128: 148-156, (2018).

Performance of NIST SRM® 2917 with 13 Recreational Water Quality Monitoring qPCR Assays

공공데이터포털

qPCR assay measurements using the pilot reference material and NIST SRM 2917. This dataset is associated with the following publication: Willis, J., M. Sivaganesan, R. Haugland, J. Kralj, S. Servetas, M.E. Hunter, S.A. Jackson, and O. Shanks. Performance of NIST SRM® 2917 with 13 Recreational Water Quality Monitoring qPCR Assays. WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 212: 118114, (2022).

Performance of NIST SRM® 2917 with 13 Recreational Water Quality Monitoring qPCR Assays

공공데이터포털

qPCR assay measurements using the pilot reference material and NIST SRM 2917. This dataset is associated with the following publication: Willis, J., M. Sivaganesan, R. Haugland, J. Kralj, S. Servetas, M.E. Hunter, S.A. Jackson, and O. Shanks. Performance of NIST SRM® 2917 with 13 Recreational Water Quality Monitoring qPCR Assays. WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 212: 118114, (2022).