Data available in Science Hub includes measurements used to generate Figures 1, 2, S3, and S4. All other data is reported in the manuscript text. This dataset is associated with the following publication: Li, X., L. Peed, M. Sivaganesan, C. Kelty, C. Nietch, and O. Shanks. Evidence of Genetic Fecal Marker Interactions between Water Column and Periphyton in Artificial Streams. ACS Omega. American Chemical Society, Washington, DC, USA, 3(8): 10107-10113, (2018).

A data package consisting of diatom, total phosphorus, and related data collected in Big Cypress National Preserve as part of the SFCN Periphyton Vital Signs protocol. Periphyton is a microbial mat community that forms the base of the aquatic food web in South Florida wetlands such as the marshes in Everglades National Park (EVER) and the Big Cypress National Preserve (BICY). It is a vital component of these wetlands, contributing to critical functions such as primary production, nutrient cycling, and soil production and stabilization. Periphyton is sensitive to changes in hydropattern (water quantity and duration) and water quality. Changes to these ecosystem processes can quickly alter periphyton community structure (species composition and relative abundance), which in turn can create cascading effects through higher trophic levels. Thus, periphyton is an early and integrative indicator of local and regional changes to hydrology. The South Florida/Caribbean Network has implemented the monitoring of periphyton communities in Big Cypress National Preserve to assess the impact of hydrologic and water quality changes on the diatom community structure and total phosphorous (TP) concentrations of the periphyton mats. The specific monitoring objectives are: 1) Identify any basins in the northwest section of Big Cypress National Preserve where periphyton community structure and periphyton TP content are different from an oligotrophic (low-nutrient) and unimpacted community signal. 2) Document any temporal and/or spatial changes in the periphyton community structure and periphyton TP content showing progression towards an oligotrophic, unimpacted condition or a eutrophic (high-nutrient), impacted condition. This dataset includes all data collected (both pilot and standard) between 2008 and 2025. 2025 sites are listed in this release alongside their field data, but lab results have yet to be received for these sites at this time.

Dataset contains measured concentrations of PFAS analytes and limits of detection/quantification for 37 total samples. There are 82 total PFAS analytes (7 non-extracted internal standards, 24 extracted internal standards, and 51 target native analytes) and each individual sample has been quantified for all 82 of these compounds. Samples are extracts of plant tissues from three sources: Tucker Prairiegrass (a mix of orchardgrass and switchgrass collected from Tucker Prairie outside of Columbia, MO), dried switchgrass hay, and whole switchgrass vegetation (the latter two provided by Jason Masoner from the USGS Oklahoma-Texas Water Science Center). These three sample types have been extracted using three different extraction procedures: the novel hypothesized method of PFAS extraction in plants, a method based on a procedure reported in Nassazzi, 2022, and a third method based on a procedure reported in Zhao, 2024 as part of an Agilent Technologies Application Note. This study evaluates the extraction performance of each of these three methods across different plant matrices.

Data from small lab scale tests conducted at ECBC. It contains efficacy data as well as data on env conditions such as temperature, RH, and hydrogen peroxide vapor concentration. This dataset is associated with the following publication: Wood, J., W. Calfee, S. Ryan, L. Mickelsen, M. Clayton, and V. Rastogi. A Simple Decontamination Approach Using Hydrogen Peroxide Vapor for Bacillus anthracis Spore Inactivation. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, USA, 121(6): 1603-1615, (2016).

A data package consisting of diatom, total phosphorus, vegetation, and related data collected in Big Cypress National Preserve as part of the SFCN Periphyton Vital Signs protocol. This hydrologic year was collected before the 2019 publication of the protocol so it represents pilot data, but the methods were largely consistent with what was published. Periphyton is a microbial mat community that forms the base of the aquatic food web in South Florida wetlands such as the marshes in Everglades National Park (EVER) and the Big Cypress National Preserve (BICY). It is a vital component of these wetlands, contributing to critical functions such as primary production, nutrient cycling, and soil production and stabilization. Periphyton is sensitive to changes in hydropattern (water quantity and duration) and water quality. Changes to these ecosystem processes can quickly alter periphyton community structure (species composition and relative abundance), which in turn can create cascading effects through higher trophic levels. Thus, periphyton is an early and integrative indicator of local and regional changes to hydrology. The South Florida/Caribbean Network has implemented the monitoring of periphyton communities in Big Cypress National Preserve to assess the impact of hydrologic and water quality changes on the diatom community structure and total phosphorous (TP) concentrations of the periphyton mats. The specific monitoring objectives are: 1) Identify any basins in the northwest section of Big Cypress National Preserve where periphyton community structure and periphyton TP content are different from an oligotrophic (low-nutrient) and unimpacted community signal. 2) Document any temporal and/or spatial changes in the periphyton community structure and periphyton TP content showing progression towards an oligotrophic, unimpacted condition or a eutrophic (high-nutrient), impacted condition.

A data package consisting of diatom, total phosphorus, vegetation, and related data collected in Big Cypress National Preserve as part of the SFCN Periphyton Vital Signs protocol. This hydrologic year was collected before the 2019 publication of the protocol so it represents pilot data, but the methods were largely consistent with what was published. Periphyton is a microbial mat community that forms the base of the aquatic food web in South Florida wetlands such as the marshes in Everglades National Park (EVER) and the Big Cypress National Preserve (BICY). It is a vital component of these wetlands, contributing to critical functions such as primary production, nutrient cycling, and soil production and stabilization. Periphyton is sensitive to changes in hydropattern (water quantity and duration) and water quality. Changes to these ecosystem processes can quickly alter periphyton community structure (species composition and relative abundance), which in turn can create cascading effects through higher trophic levels. Thus, periphyton is an early and integrative indicator of local and regional changes to hydrology. The South Florida/Caribbean Network has implemented the monitoring of periphyton communities in Big Cypress National Preserve to assess the impact of hydrologic and water quality changes on the diatom community structure and total phosphorous (TP) concentrations of the periphyton mats. The specific monitoring objectives are: 1) Identify any basins in the northwest section of Big Cypress National Preserve where periphyton community structure and periphyton TP content are different from an oligotrophic (low-nutrient) and unimpacted community signal. 2) Document any temporal and/or spatial changes in the periphyton community structure and periphyton TP content showing progression towards an oligotrophic, unimpacted condition or a eutrophic (high-nutrient), impacted condition.

Dataset includes data from each experiment conducted in the pilot-scale testing. Each sheet of the Excel file pertains to each test. A data dictionary is included in the first sheet. In each sheet there are microbiological data (colony forming units) for each test and positive control coupon used in the study. Also shown is the calculation of decontamination efficacy (log10 reduction). This dataset is associated with the following publication: Wood, J., W. Calfee, S. Ryan, L. Mickelsen, M. Clayton, and V. Rastogi. A Simple Decontamination Approach Using Hydrogen Peroxide Vapor for Bacillus anthracis Spore Inactivation. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, USA, 121(6): 1603-1615, (2016).

These data include sample information and gene sequence reads for amplicon sequence variants from a study of freezing and storage duration effects on diatom-environment relationships. This dataset is associated with the following publication: Smucker, N., E. Pilgrim, C. Nietch, and C. Carpenter. Freezing periphyton samples and storage duration do not affect the use of diatom DNA metabarcoding to determine effects of stressors on streams. ENVIRONMENTAL MONITORING AND ASSESSMENT. Springer, New York, NY, USA, 197: 1360, (2025).