The data were gathered as part of a long-term, seasonal assessment of estrogenicity in selected locations within Pennsylvania from 2012-2017. Data were collected over this time frame to determine seasonal and annual changes in surface water estrogenicity.Estrogen is reported as estrogen equivalents (EEQ) as determined by a bioluminescent yeast estrogen screen. A total of 193 grab water samples were collected during this time of which 41% had EEQ above the limit of detection. The maximum estrogen equivalents (EEQ) measured was 5.44 ng EEQ/ L, while the median concentration was 0.44 ng/L. POCIS samplers were deployed at a number of sites to determine time-weighted estrogenicity between 2014 and 2017. A total of 150 POCIS samples were deployed over this time of which 77% had EEQ above the limit of detection.. The maximum estrogen equivalents (EEQ) measured was 12 ng EEQ/ POCIS.

Intersex in small- and largemouth bass has been observed in the upper Chesapeake Bay Watershed since 2003. This observation is indicative of exposure to estrogenic endocrine disrupting chemicals. In order to complement biological data sets and better identify the spatial and temporal nature of this end-point, discrete grab water samples were collected throughout the upper Chesapeake Bay Watershed for the evaluation of estrogenic activity. Data reported here are estrogenicity values derived from bioassay screening of OASIS-HLB extracts discrete grab water samples. Estrogenicity was determined using a bioluminescent yeast estrogen screen. Here we report estrogenicity data for selected sites in the Chesapeake Bay water shed collected between 2008 and 2011.

Intersex in small- and largemouth bass has been observed in the upper Chesapeake Bay Watershed since 2003. This observation is indicative of exposure to estrogenic endocrine disrupting chemicals. In order to complement biological data sets and better identify the spatial and temporal nature of this end-point, discrete grab water samples were collected throughout the upper Chesapeake Bay Watershed for the evaluation of estrogenic activity. Data reported here are estrogenicity values derived from bioassay screening of OASIS-HLB extracts discrete grab water samples. Estrogenicity was determined using a bioluminescent yeast estrogen screen. Here we report estrogenicity data for selected sites in the Chesapeake Bay water shed collected between 2008 and 2011.

The data were gathered as a preliminary assessment of estrogenicity under base-flow conditions at over 100 sites (lakes and streams) throughout New Jersey followed by more targeted sampling of smallmouth bass at nine sites with varying levels of estrogenicity. In 2016, 102 sites (lakes and streams) for the preliminary estrogenicity assessment were selected utilizing compiled results of previous monitoring studies (chemical and biological), current sampling networks, and other areas of concern based on input from stakeholders. Discrete grab surface water samples were collected under base-flow conditions in the fall of 2016 and analyzed for levels of estrogenicity using a bioluminescent yeast screen. Water samples for estrogenicity were also collected from the nine health sites in April/May of 2017 in combination with fish sampling and again in November 2017 under base flow conditions. In the spring of 2017, 20 adult smallmouth bass were collected from each of the nine sites (two river and seven reservoir sites). Fish were collected by boat electroshocking, euthanized in the field, weighed, measured, a blood sample collected and a necropsy completed that included documenting any visible abnormalities and collecting tissues for histopathology. Plasma obtained from blood samples was analyzed for vitellogenin, a yolk precursor widely used as an indicator of estrogenic exposure in immature and male fishes. Five to seven sections of testes were processed for microscopic analyses, embedded in paraffin, sectioned at 5 µm, stained with hematoxylin and eosin. The presence of testicular oocytes was noted and the severity rated from 1 to 4 based on number and arrangement of oocytes.

Compares results of bioassay with hormones measured using analytical chemistry. This dataset is associated with the following publication: Conley, J., H. Mash , N. Evans , K. Schenck , L. Rosenblum, S. Glassmeyer , E.T. Furlong, D.W. Kolpin, and V. Wilson. Comparison of in vitro estrogenic activity and estrogen concentrations in source and treated waters from 25 U.S. drinking water treatment plants. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, N/A, (2016).

This USGS data release contains station and laboratory method information and geospatial information, as well as concentration results for inorganic and organic compounds and bioluminescent yeast estrogen screen and transgenic zebrafish embryo estrogren bioassay, analyzed at 38 sites in 25 states as part of the Chemical Mixtures and Environmental Effects Pilot Study, 2012-2014.

This data release supports the following publication: Hittle, Elizabeth, 2017, Longshore Water-Current Velocity and the Potential for Transport of Contaminants: A Pilot Study in Lake Erie from Walnut Creek to Presque Isle State Park Beaches, Erie, Pennsylvania, June and August 2015: U.S. Geological Survey Open-File Report 2016–1206 126 p., https://doi.org/10.3133/ofr20161206. This data set contains water quality data (temperature, specific conductance, and turbidity) measured and recorded on June 25, 2015 with a YSI EXO probe and depth measured and recorded by a design analysis 700H pressure transducer. The data was collected along select transects at ~300 meter spacing by moving the probe down and up throughout the water column. Data is provided only when the probe was moving from the bottom to the top of the water column as the data recorded from top to bottom was found to be biased. Data was recorded at 1 second intervals.

This data release supports the following publication: Hittle, Elizabeth, 2017, Longshore Water-Current Velocity and the Potential for Transport of Contaminants: A Pilot Study in Lake Erie from Walnut Creek to Presque Isle State Park Beaches, Erie, Pennsylvania, June and August 2015: U.S. Geological Survey Open-File Report 2016–1206 126 p., https://doi.org/10.3133/ofr20161206. This data set contains water quality data (temperature, specific conductance, and turbidity) measured and recorded on June 25, 2015 with a YSI EXO probe and depth measured and recorded by a design analysis 700H pressure transducer. The data was collected along select transects at ~300 meter spacing by moving the probe down and up throughout the water column. Data is provided only when the probe was moving from the bottom to the top of the water column as the data recorded from top to bottom was found to be biased. Data was recorded at 1 second intervals.

This data release supports the following publication: Hittle, Elizabeth, 2017, Longshore Water-Current Velocity and the Potential for Transport of Contaminants: A Pilot Study in Lake Erie from Walnut Creek to Presque Isle State Park Beaches, Erie, Pennsylvania, June and August 2015: U.S. Geological Survey Open-File Report 2016–1206 126 p., https://doi.org/10.3133/ofr20161206. This data set contains water quality data (temperature, specific conductance, and turbidity) measured and recorded on June 24, 2015 with a YSI EXO probe and depth measured and recorded by a design analysis 700H pressure transducer. The data was collected along select transects at ~300 meter spacing by moving the probe down and up throughout the water column. Data is provided only when the probe was moving from the bottom to the top of the water column as the data recorded from top to bottom was found to be biased. Data was recorded at 5 second intervals except for transects 17 and 24 which were collected at 1 second intervals. The depth data for transects 4 and 6 are not available due to user error.

This data release supports the following publication: Hittle, Elizabeth, 2017, Longshore Water-Current Velocity and the Potential for Transport of Contaminants: A Pilot Study in Lake Erie from Walnut Creek to Presque Isle State Park Beaches, Erie, Pennsylvania, June and August 2015: U.S. Geological Survey Open-File Report 2016–1206 126 p., https://doi.org/10.3133/ofr20161206. This data set contains water quality data (temperature, specific conductance, and turbidity) measured and recorded on June 24, 2015 with a YSI EXO probe and depth measured and recorded by a design analysis 700H pressure transducer. The data was collected along select transects at ~300 meter spacing by moving the probe down and up throughout the water column. Data is provided only when the probe was moving from the bottom to the top of the water column as the data recorded from top to bottom was found to be biased. Data was recorded at 5 second intervals except for transects 17 and 24 which were collected at 1 second intervals. The depth data for transects 4 and 6 are not available due to user error.