This dataset contains single nucleotide polymorphism (SNP) information for aquatic insect species collected in tributaries of the Colorado River in Grand Canyon (Arizona, USA), as well as SNP information for individuals collected from reference reaches of the Upper Colorado River Basin in Utah. This dataset focuses specifically on three species that were common and widely distributed throughout tributary streams in Grand Canyon: a mayfly (Fallceon quilleri), a caddisfly (Hydrospyche oslari), and a water strider (Rhagovelia distincta).

These data were compiled for a mtDNA (CO1 gene) analysis of net-spinning caddisflies (Hydropsyche oslari) in the Colorado River Basin of the western United States. They were incorporated in a study that investigated the genetic diversity and population structure of H. oslari relative to the river network structure in the Upper and Lower Colorado River Basins. The data were collected from 2015 to 2016 through a collaboration with river runners. These citizen scientists collected insects by deploying light traps for one hour each night of their expedition. Light trap contents were preserved in ethanol on site and returned to the Southwest Biological Science Center in Flagstaff, Arizona for analysis in the laboratory.

These data were compiled for a mtDNA (CO1 gene) analysis of net-spinning caddisflies (Hydropsyche oslari) in the Colorado River Basin of the western United States. They were incorporated in a study that investigated the genetic diversity and population structure of H. oslari relative to the river network structure in the Upper and Lower Colorado River Basins. The data were collected from 2015 to 2016 through a collaboration with river runners. These citizen scientists collected insects by deploying light traps for one hour each night of their expedition. Light trap contents were preserved in ethanol on site and returned to the Southwest Biological Science Center in Flagstaff, Arizona for analysis in the laboratory.

These data show multilocus genotypes, banding age, and territory for coastal cactus wrens (Campylorhynchus brunneicapillus) sampled in coastal Southern California between 2009 and 2019.

These data were compiled to provide land-management relevant information on the native and nonnative subspecies (subsp.) of Phragmites australis (subsp. americanus and subsp. australis, respectively) in Glen Canyon National Recreation Area (NRA), Grand Canyon National Park (NP), Arches National Park, and Bill Williams National Wildlife Refuge. Specifically, the goals of this work were to determine the extent and distribution of Phragmites australis subspecies in the Glen and Grand Canyon regions, evaluate if P.a. subsp. americanus can be reliably distinguished from P.a. subsp. australis using morphological characters in this region, and determine if P.a. subsp. americanus exhibits genetic structure in the study area. The objectives of our study were to determine how prevalent the nonnative Phragmites is in the study area and support native plant material development for restoration activities in Glen Canyon NRA and Grand Canyon NP. These data represent real-time PCR cycle threshold values (Ct scores) for two target regions, nuclear microsatellite data for seven loci, and morphological data. These data were collected for 84 sites including five nonnative Phragmites locations and 79 native Phragmites locations. Tissue samples were mostly collected along the Colorado River between Glen Canyon Dam and Lake Mead, around Lake Powell, and in tributaries to the Colorado River from 2021 through 2024. Tissue samples and herbarium collections were collected from one to fifteen ramets across each stand, where multiple samples from one stand were spread across its full area. Reference samples for both the native and nonnative Phragmites, Phragmites australis subsp. berlandieri, and a hybrid of P.a. australis and P.a. americanus were acquired and included in analyses. Samples were dried and total genomic DNA was extracted using Qiagen DNeasy Plant MiniKits. Real-time polymerase chain reactions (PCR) with two target regions, AMER and AMAU, were used to identify nonnative Phragmites individuals (Lindsay et al, 2023). We then amplified 7 microsatellite loci (Saltonstall, 2003; Meyerson and others, 2010) using PCR and analyzed the fragments on an ABI 3730XL Genetic Analyzer with GeneScan LIZ500 internal size standard. Although P. australis is polyploid, these loci all had no more than two alleles, so were treated as diploid data. We then collected field and lab data on morphological characters for the stands we genetically tested, following previously described diagnostic characters (Swearington and Saltonstall, 2012; McTavish et al, 2023) These data can be used to evaluate if a stand of Phragmites is nonnative, native, or a hybrid of the two. It can also be used to determine genetic diversity and structure across the sampled stands. Finally, they can be used to assess morphological variability in native Phragmites stands across the region.

These data show the multilocus genotypes, gender, and midline carapace length (MCL) for desert tortoises (Gopherus agassizii) sampled in the central portion of the Mojave desert tortoise range.

These data show the multilocus genotypes, gender, and midline carapace length (MCL) for desert tortoises (Gopherus agassizii) sampled in the central portion of the Mojave desert tortoise range.

These data represent nuclear microsatellite data collected from four riparian plant species that occur in and around Grand Canyon National Park: Populus fremontii (POFR), Salix gooddingii (SAGO), Salix exigua (SAEX), and Prosopis glandulosa (PRGL). These data were collected for population genetic analysis to help inform native plant material development in Grand Canyon National Park. Leaf samples were collected at sites spanning 470 km of the Colorado River between Glen Canyon Dam and Lake Mead and in tributaries. Known revegetation areas were not sampled. We aimed to collect leaf tissue from at least 15 individuals at each sample site. If there were fewer than 15 individuals per species at a site, leaf tissue was collected from every individual. Leaves were immediately dried and stored in silica gel. For P. fremontii, S. gooddingii, and P. glandulosa, total genomic DNA was extracted from dried leaf tissue using a high-molecular weight protocol with modifications. For S. exigua, DNeasy Plant Minikits were used. We amplified 9, 9, 11, and 13 loci for P. fremontii, S. gooddingii, S. exigua, and P. glandulosa, respectively. All loci were amplified using polymerase chain reaction (PCR) and fragment analysis processed on an ABI 3730xl Genetic Analyzer with GeneScan LIZ500 internal size standard. Allele fragment sizes were scored using GeneMarker v2.2.0. Loci that were missing more than 5% of values, were not polymorphic, or could not be reliably scored were omitted. Nine loci were included for P. fremontii, six for S. gooddingii, eight for P. glandulosa, and nine for S. exigua. All species are diploid, so each microsatellite locus has two values. Associated with the microsatellite data are labels delineating what collection site each individual came from, which genetic group each individual was assigned to during statistical analyses, and information about those sites. Specifically, sites are noted as being on the Colorado River or on a tributary to it, and inside or outside of the canyon rims.

These data represent nuclear microsatellite data collected from four riparian plant species that occur in and around Grand Canyon National Park: Populus fremontii (POFR), Salix gooddingii (SAGO), Salix exigua (SAEX), and Prosopis glandulosa (PRGL). These data were collected for population genetic analysis to help inform native plant material development in Grand Canyon National Park. Leaf samples were collected at sites spanning 470 km of the Colorado River between Glen Canyon Dam and Lake Mead and in tributaries. Known revegetation areas were not sampled. We aimed to collect leaf tissue from at least 15 individuals at each sample site. If there were fewer than 15 individuals per species at a site, leaf tissue was collected from every individual. Leaves were immediately dried and stored in silica gel. For P. fremontii, S. gooddingii, and P. glandulosa, total genomic DNA was extracted from dried leaf tissue using a high-molecular weight protocol with modifications. For S. exigua, DNeasy Plant Minikits were used. We amplified 9, 9, 11, and 13 loci for P. fremontii, S. gooddingii, S. exigua, and P. glandulosa, respectively. All loci were amplified using polymerase chain reaction (PCR) and fragment analysis processed on an ABI 3730xl Genetic Analyzer with GeneScan LIZ500 internal size standard. Allele fragment sizes were scored using GeneMarker v2.2.0. Loci that were missing more than 5% of values, were not polymorphic, or could not be reliably scored were omitted. Nine loci were included for P. fremontii, six for S. gooddingii, eight for P. glandulosa, and nine for S. exigua. All species are diploid, so each microsatellite locus has two values. Associated with the microsatellite data are labels delineating what collection site each individual came from, which genetic group each individual was assigned to during statistical analyses, and information about those sites. Specifically, sites are noted as being on the Colorado River or on a tributary to it, and inside or outside of the canyon rims.

This data release comprises a dataset that contains sample collection information and microsatellite genotypes, and another dataset that contains single nucleotide polymorphism (SNP) genotypes with sample collection information for populations of white-tailed ptarmigan across the species' range. There is also an additional file (accession numbers.xlsx) linking samples to accession numbers in Genbank. This data was collected in order to address the following: The delineation of intraspecific units that are evolutionarily and demographically distinct is an important step in the development of species-specific management plans. Neutral genetic variation has served as the primary data source for delineating units for conservation, but with recent advances in genomic technology, we now have an unprecedented ability to utilize information about neutral and adaptive variation across the entire genome. Here, we use traditional genetic markers (microsatellites) and a newer reduced-representation genomic approach (single nucleotide polymorphisms) to delineate distinct groups of white-tailed ptarmigan (Lagopus leucura), an alpine-obligate species that is distributed in naturally fragmented habitats from Alaska to New Mexico.