Microsatellite Genetic Data for Sea Sandwort (Honckenya peploides) and Merckia (Wilhelmsia physodes), Alaska, 2009-2016
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This dataset contains sampling information and genetic data of 12 novel microsatellite loci developed and polymorphic in the Sea Sandwort (Honckenya peploides) and screened in Merckia (Wilhelmsia physodes) from Alaska. The allele sizes for the 12 microsatellite loci developed in this study are included for two populations of Sea Sandwort (Fossil Beach, Kodiak and Egg Bay, Atka) and one population of Merckia (upper Coleen River, Brooks Range).
Microsatellite Genetic Data for Sea Sandwort (Honckenya peploides) and Merckia (Wilhelmsia physodes), Alaska, 2009-2016
공공데이터포털
This dataset contains sampling information and genetic data of 12 novel microsatellite loci developed and polymorphic in the Sea Sandwort (Honckenya peploides) and screened in Merckia (Wilhelmsia physodes) from Alaska. The allele sizes for the 12 microsatellite loci developed in this study are included for two populations of Sea Sandwort (Fossil Beach, Kodiak and Egg Bay, Atka) and one population of Merckia (upper Coleen River, Brooks Range).
Functional gene microarray data from cold-water corals (Acanthogorgia spp., Desmophyllum dianthus, Desmophyllum pertusum, and Enallopsammia profunda) from the Atlantic Ocean off the Southeast Coast of the United States–Raw Data and Sample Site Information
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The files in this data release (Kellogg and Voelschow, 2023) contain normalized microarray probe intensity values from GeoChip 5.0S microarrays referenced in the journal article entitled “Functional gene composition and metabolic potential of deep-sea coral-associated microbial communities” by Pratte and others (2023). The GeoChip 5.0S microarrays, provided by Glomics Inc., contain 57,498 oligonucleotide probes that target 383 microbial (archaeal, bacterial, and fungal) genes and cover 151,797 coding sequences within the following metabolic categories: carbon, sulfur, and nitrogen cycling, as well as metal homeostasis, antibiotic resistance, and contaminant degradation. One microarray was run per coral (number of samples [n] = 11), using 400 nanograms (ng) of DNA extracted from the sample, plus a microarray run as a reagent blank (n = 1). The coral samples included one Acanthogorgia aspera, one Acanthogorgia spissa, three Desmophyllum dianthus, three Desmophyllum pertusum (formerly Lophelia pertusa) and three Enallopsammia profounda species. Corals were collected during two research cruises: the first in August 2018 and the second in April 2019, from six sites offshore of the southeastern coast of the United States in water depths ranging from 296–1567 meters (m). Coral samples were flash frozen in liquid nitrogen on the ship and stored at -80 degrees Celsius (°C) until processed. Extraction of deoxyribose nucleic acid (DNA) from the coral samples and kit blank occurred on August 5, 2019, at the Coral Microbial Ecology Laboratory in St. Petersburg, Florida (FL) using a QIAGEN DNeasy PowerBiofilm kit. Then, DNA samples were sent to Glomics Inc. on August 6, 2019, for application of GeoChip 5.0S microarrays. For more information, please see the README file and metadata files.
Laysan Teal (Anas laysanensis) Microsatellite DNA Data, Laysan Island 1999-2009, Midway Atoll 2007-2010
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The geographically closed, non-migratory populations of endangered Laysan Teal (Anas laysanensis) were sampled in the wild. This species was once widespread across the Hawaiian archipelago, but became isolated on Laysan Island (415 ha) from the mid-1800s until 2004 when a translocation to Midway Atoll (596 ha) was undertaken to reduce extinction risks. We compared genetic diversity and quantified variation at microsatellite loci sampled from 229 individuals from the wild populations at Laysan Island (1999–2009) and Midway Atoll (2007–2010; n = 133 Laysan, n = 96 Midway birds). We identified polymorphic markers by screening nuclear microsatellites (N = 83). Low nuclear variation was detected, consistent with the species’ insular isolation and historical bottleneck. Six of 83 microsatellites were polymorphic.