Data were collected from Xenopus laevis tadpoles exposed to clothianidin and thiamethoxam in two concentrations each, 100 ppm and 20 ppm. Gene expression was generated from brains and livers, and liver cells provided metabolic activity data.

Xenopus laevis tadpoles were held in 4 replicate vivaria for each of 4 treatments of neonicotinoid pesticides and one control treatment for at least one month (Control media, thiamethoxam high concentration of 100 ppm, thiamethoxam low concentrations of 20 ppm, clothianidin high concentrations of 100 ppm, and clothianidin low concentration of 20 ppm). Water was sampled for chemical testing on Day 33. Between Day 1 and 44, instantaneous measures of length were collected on a random selection of tadpoles from each vivarium. On Day 44, tadpole length (mm), weight, and developmental stage (Nieuwkoop-Faber staging) were measured for all tadpoles in all vivaria (three of the measured individuals from each replicate treatment group (total n=12 for each treatment) were vivisected and liver, brain, and somatic tissue frozen in RNA/DNA shield for qPCR analyses for another study). On Day 44, tadpoles (n=5) which had reached NF stage 57 were transferred to other vivaria and remained in their original treatment solution through Day 76; these animals were monitored until metamorphosis.

GC/MS metabolomic data from livers of amphibians exposed to pesticides. This dataset is associated with the following publication: Glinski, D., T. Purucker, R. Van Meter, M. Black, and M. Henderson. Endogenous and exogenous biomarker analysis in terrestrial phase amphibians (Lithobates sphenocephala) following dermal exposure to pesticide mixtures. ENVIRONMENTAL CHEMISTRY. CSIRO Publishing, Collingwood Victoria, AUSTRALIA, 16(1): 55-67, (2019).

GC/MS metabolomic data from livers of amphibians exposed to pesticides. This dataset is associated with the following publication: Glinski, D., T. Purucker, R. Van Meter, M. Black, and M. Henderson. Endogenous and exogenous biomarker analysis in terrestrial phase amphibians (Lithobates sphenocephala) following dermal exposure to pesticide mixtures. ENVIRONMENTAL CHEMISTRY. CSIRO Publishing, Collingwood Victoria, AUSTRALIA, 16(1): 55-67, (2019).

Retention time and abundances from GC/MS analysis of amphibian livers exposed to pesticide, predation or combined stresses. This dataset is associated with the following publication: Henderson, M., M.N. Snyder, D. Glinski, and T. Purucker. Differentiating metabolomic responses of amphibians to multiple stressors. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 838(3): 155666, (2022).

Retention time and abundances from GC/MS analysis of amphibian livers exposed to pesticide, predation or combined stresses. This dataset is associated with the following publication: Henderson, M., M.N. Snyder, D. Glinski, and T. Purucker. Differentiating metabolomic responses of amphibians to multiple stressors. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 838(3): 155666, (2022).

Amphibian metabolite data used in Snyder, M.N., Henderson, W.M., Glinski, D.G., Purucker, S. T., 2017. Biomarker analysis of american toad (Anaxyrus americanus) and grey tree frog (Hyla versicolor) tadpoles following exposure to atrazine. Aquatic Toxicology, 182(184-193). doi: 10.1016/j.aquatox.2016.11.018. This dataset is associated with the following publication: Snyder, M., M. Henderson, D. Glinski, and T. Purucker. Biomarker analysis of American toad (Anaxyrus americanus) and grey tree frog (Hyla versicolor) tadpoles following exposure to atrazine.. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 182: 184-193, (2017).

Understanding biotransformation pathways in aquatic species is an integral part of ecological risk assessment with respect to the potential bioactivation of chemicals to more toxic metabolites. The long-range goal is to gain sufficient understanding of fish metabolic transformation reactions to be able to accurately predict fish xenobiotic metabolism. While some metabolism data exist, there are few fish in vivo exposure studies where metabolites have been identified and the metabolic pathways proposed. Previous biotransformation work has focused on in vitro studies which have the advantage of high throughput but may have limited metabolic capabilities, and in vivo studies which have full metabolic capacity but are low throughput. An aquatic model system with full metabolic capacity in which a large number of chemicals could be tested would be a valuable tool. The current study evaluated the ex vivo rainbow trout liver slice model, which has the advantages of high throughput as found in vitro models and non-dedifferentiated cells and cell to cell communication found in in vivo systems. The pesticide diazinon, which has been previously tested both in vitro and in vivo in a number of mammalian and aquatic species including rainbow trout, was used to evaluate the ex vivo slice model as a tool to study biotransformation pathways. While somewhat limited by the analytical chemistry method employed, results of the liver slice model, mainly that hydroxypyrimidine was the major diazinon metabolite, are in line with the results of previous rainbow trout in vivo studies. Therefore, the rainbow trout liver slice model is a useful tool for the study of metabolism in aquatic species. This dataset is associated with the following publication: Tapper, M., J. Serrano, P. Schmieder, D. Hammermeister, and R. Kolanczyk. Metabolism of diazinon in rainbow trout liver slices. Applied In Vitro Toxicology. Mary Ann Liebert, Inc., Larchmont, NY, USA, 4(1): 13-23, (2018).

Understanding biotransformation pathways in aquatic species is an integral part of ecological risk assessment with respect to the potential bioactivation of chemicals to more toxic metabolites. The long-range goal is to gain sufficient understanding of fish metabolic transformation reactions to be able to accurately predict fish xenobiotic metabolism. While some metabolism data exist, there are few fish in vivo exposure studies where metabolites have been identified and the metabolic pathways proposed. Previous biotransformation work has focused on in vitro studies which have the advantage of high throughput but may have limited metabolic capabilities, and in vivo studies which have full metabolic capacity but are low throughput. An aquatic model system with full metabolic capacity in which a large number of chemicals could be tested would be a valuable tool. The current study evaluated the ex vivo rainbow trout liver slice model, which has the advantages of high throughput as found in vitro models and non-dedifferentiated cells and cell to cell communication found in in vivo systems. The pesticide diazinon, which has been previously tested both in vitro and in vivo in a number of mammalian and aquatic species including rainbow trout, was used to evaluate the ex vivo slice model as a tool to study biotransformation pathways. While somewhat limited by the analytical chemistry method employed, results of the liver slice model, mainly that hydroxypyrimidine was the major diazinon metabolite, are in line with the results of previous rainbow trout in vivo studies. Therefore, the rainbow trout liver slice model is a useful tool for the study of metabolism in aquatic species. This dataset is associated with the following publication: Tapper, M., J. Serrano, P. Schmieder, D. Hammermeister, and R. Kolanczyk. Metabolism of diazinon in rainbow trout liver slices. Applied In Vitro Toxicology. Mary Ann Liebert, Inc., Larchmont, NY, USA, 4(1): 13-23, (2018).

Files contain summary reports of chemical and Mass Spectrometry raw and analyzed data used as basis for plot generation and text discussions in the Cyclic phenone metabolism manuscript. File dataset includes a Definition Xcel file listing the supporting files in set. Specifically, the dataset includes individual chemical slice exposure Xcel data summaries for the model cyclic phenones DPK, CBP and CPK as well as well as hepatocyte cytosol exposure data to the same chemicals. Each file contains data organized in labelled tabs that includes General Experimental information, Quantitative and qualitative methods used to process/analyze the raw data for parent and chemical metabolites, chemical mass balances and conclusions. Power point files with the summarized data were presented in the manuscript's Supplemental data. This dataset is associated with the following publication: Serrano, J., M. Tapper, R. Kolanczyk, B. Sheedy, T. Lahren, D. Hammermeister, J. Denny, M. Hornung, A. Kubatova, P. Kosian, J. Voelker, and P. Schmieder. Metabolism of cyclic phenones in rainbow trout in vitro assays. XENOBIOTICA. Taylor & Francis, Inc., Philadelphia, PA, USA, 50(2): 115-131, (2019).