Xenopus laevis tadpoles were held in 4 replicate vivaria for each of 4 treatments of neonicotinoid pesticides and one control treatment for at least one month (Control media, thiamethoxam high concentration of 100 ppm, thiamethoxam low concentrations of 20 ppm, clothianidin high concentrations of 100 ppm, and clothianidin low concentration of 20 ppm). Water was sampled for chemical testing on Day 33. Between Day 1 and 44, instantaneous measures of length were collected on a random selection of tadpoles from each vivarium. On Day 44, tadpole length (mm), weight, and developmental stage (Nieuwkoop-Faber staging) were measured for all tadpoles in all vivaria (three of the measured individuals from each replicate treatment group (total n=12 for each treatment) were vivisected and liver, brain, and somatic tissue frozen in RNA/DNA shield for qPCR analyses for another study). On Day 44, tadpoles (n=5) which had reached NF stage 57 were transferred to other vivaria and remained in their original treatment solution through Day 76; these animals were monitored until metamorphosis.

Data were collected from Xenopus laevis tadpoles exposed to clothianidin and thiamethoxam in two concentrations each, 100 ppm and 20 ppm. Gene expression was generated from brains and livers, and liver cells provided metabolic activity data.

These data are represented in the tables and graphs in the journal article, Antiandrogenic effects following multiple life stage exposure to the fungicide prochloraz in Xenopus laevis by JT Haselman et al. This dataset is associated with the following publication: Haselman, J., P. Kosian, J. Korte, A. Olmstead, and S. Degitz. Effects of multiple life stage exposure to the fungicide prochloraz in Xenopus laevis: Manifestations of antiandrogenic and other modes of toxicity. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 199: 240-251, (2018).

These data are represented in the tables and graphs in the journal article, Antiandrogenic effects following multiple life stage exposure to the fungicide prochloraz in Xenopus laevis by JT Haselman et al. This dataset is associated with the following publication: Haselman, J., P. Kosian, J. Korte, A. Olmstead, and S. Degitz. Effects of multiple life stage exposure to the fungicide prochloraz in Xenopus laevis: Manifestations of antiandrogenic and other modes of toxicity. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 199: 240-251, (2018).

Dataset consists of 4, 7, 10, and 14-day responses of laboratory cultured mayflies (Neocloeon triangulifer) to sodium chloride exposure under different test condition scenarios. Responses were measured as mortality and growth (length). Water quality and analytical chemistry results associated with toxicity data are included. Experiments included 1) testing the influence of organism age on mortality and growth in 7-day tests, 2) testing the influence of test duration on mortality and growth, 3) testing the influence of temperature on mortality and growth, 4) testing the influence of nutrient amendments to mayfly test dilution water on mortality and growth

,This dataset includes data on 25 transitions of a matrix demographic model of the invasive species Vincetoxicum nigrum (L.) Moench (black swallow-wort or black dog-strangling vine) and Vincetoxicum rossicum (Kleopow) Barb. (pale swallow-wort or dog-strangling vine) (Apocynaceae, subfamily Asclepiadoideae), two invasive perennial vines in the northeastern U.S.A. and southeastern Canada. The matrix model was developed for projecting population growth rates as a result of changes to lower-level vital rates from biological control although the model is generalizable to any control tactic. Transitions occurred among the five life stages of seeds, seedlings, vegetative juveniles (defined as being in at least their second season of growth), small flowering plants (having 1–2 stems), and large flowering plants (having 3 or more stems). Transition values were calculated using deterministic equations and data from 20 lower-level vital rates collected from 2009-2012 from two open field and two forest understory populations of V. rossicum (43°51’N, 76°17’W; 42°48'N, 76°40'W) and two open field populations of V. nigrum (41°46’N, 73°44’W; 41°18’N, 73°58’W) in New York State. Sites varied in plant densities, soil depth, and light levels (forest populations). Detailed descriptions of vital rate data collection may be found in: Milbrath et al. 2017. Northeastern Naturalist 24(1):37-53. Five replicate sets of transition data obtained from five separate spatial regions of a particular infestation were produced for each of the six populations.,Note: Added new excel file of vital rate data on 12/7/2018.,

Neonicotinoids, a widely used class of insecticide, have been found in surface waters globally. They pose a risk to non-target species found in aquatic environments such as aquatic macroinvertebrates. To better understand the effects of the neonicotinoids imidacloprid, clothianidin and their mixtures on aquatic communities we ran a 30 day mesocosm test. Rock trays were colonized with natural benthic communities in the Cache La Poudre River, located in the mountains of Northern Colorado, and relocated to a laboratory experimental stream setting. In total there were 33 experimental streams: 3 controls and 30 treatments consisting of both single compound and binary compound exposures with 5 treatment levels for each exposure series. Water quality and chemistry samples were collected throughout the experiment. Larval invertebrates remaining in each experimental stream at the end of the experiment were collected, enumerated and identified to the lowest taxonomic unit practical, typically genus or species. Emergent insects were collected each day of the experiment and identified to lowest taxonomic unit. Chlorophyll a was measured in each experimental stream 3 times throughout the 30 day experiment.

These data are represented in tables and graphs in the article, "Targeted pathway-based in vivo testing using thyroperoxidase inhibition to evaluate plasma thyroxine as a surrogate metric of metamorphic success in model amphibian Xenopus laevis" by JT Haselman et al. This dataset is associated with the following publication: Haselman, J., J. Olker, P. Kosian, J. Korte, J. Swintek, J. Denny, J. Nichols, J. Tietge, M. Hornung, and S. Degitz. Targeted pathway-based in vivo testing using thyroperoxidase inhibition to evaluate plasma thyroxine as a surrogate metric of metamorphic success in model amphibian Xenopus laevis. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 175(2): 236-250, (2020).

These data are represented in tables and graphs in the article, "Targeted pathway-based in vivo testing using thyroperoxidase inhibition to evaluate plasma thyroxine as a surrogate metric of metamorphic success in model amphibian Xenopus laevis" by JT Haselman et al. This dataset is associated with the following publication: Haselman, J., J. Olker, P. Kosian, J. Korte, J. Swintek, J. Denny, J. Nichols, J. Tietge, M. Hornung, and S. Degitz. Targeted pathway-based in vivo testing using thyroperoxidase inhibition to evaluate plasma thyroxine as a surrogate metric of metamorphic success in model amphibian Xenopus laevis. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 175(2): 236-250, (2020).

These data are from two studies conducted to evaluate the performance of the draft Larval Amphibian Growth and Development Assay for incorporation into the U.S. EPA's Endocrine Disruptor Screening Program Tier II testing battery. 4-tert-octylphenol was chosen as an environmental estrogenic compound and 17-beta-trenbolone was chosen as an environmental androgenic compound. Although the effects of these model environmental endocrine disruptors are not novel, these chemicals were used essentially as positive controls to represent each of the potential modes of toxicity that could be encountered by future unknown compounds ordered to be run through the LAGDA in support of risk assessment. Endpoints evaluated were larval growth and metamorphic development, blood thyroid hormone, thyroid histopathology, juvenile growth, juvenile histopathology of the liver, gonads, kidneys and reproductive ducts. This dataset is associated with the following publication: Haselman , J., P. Kosian , J. Korte , A. Olmstead, T. Iguchi, R. Johnson , and S. Degitz. Development of the larval amphibian growth and development assay: Effects of chronic 4-tert-octylphenol or 17ß-trenbolone exposure in Xenopus laevis from embryo to juvenile. JOURNAL OF APPLIED TOXICOLOGY. John Wiley & Sons, Ltd., Indianapolis, IN, USA, 36(12): 1639-1650, (2016).