,The Leptinotarsa decemlineata genome was recently sequenced and annotated as part of the i5k pilot project by the Baylor College of Medicine. This dataset presents the Leptinotarsa decemlineata gene set BCM_v_0.5.3, which was generated computationally. RNA-Seq data was used with additional protein homology data for a MAKER automated annotation of the Leptinotarsa decemlineata genome assembly 1.0. Further annotation method details will be available in a forthcoming publication.,NOTE: This gene set is an unstable pre-release (v0.5.3), and was provided to facilitate manual curation and analyses before the official gene set is released. Gene identifiers from this gene set will likely not be maintained.,If you wish to use this dataset, please follow the Baylor College of Medicine's conditions for data use: https://www.hgsc.bcm.edu/bcm-hgsc-conditions-use,

,The Homalodisca vitripennis genome was recently sequenced and annotated as part of the i5k pilot project by the Baylor College of Medicine.,The Glassy-winged sharpshooter, GWSS, (Homalodisca vitripennis) [Hemiptera: Cicadellidae], occurs naturally within the southern United States. Once restricted to the southeastern states, it was accidentally spread across the south into California. The GWSS is a voracious feeder, and can fly long distances, preferring to feed upon cultivated crops, ie. Grapevine, fruit trees, and in the nymphal stages many weeds and grasses. The GWSS is a serious threat to the viticulture industry as the primary vector of the plant-infecting bacterium, Xylella fastidiosa, Xf. The GWSS feeds on a diverse number of plants, during which the bacteria can infect many tree fruit, nut, vine, and woody ornamental crops. Glassy-winged Sharpshooter adults are ½ inch (13mm) long being fairly large for the Sharpshooter leafhopper family of insects. Sharpshooters use an ovipositor to lay eggs inside of the underside of leaves. The Sharpshooter will lay its eggs on almost any plant including cactus. The egg masses are usually composed of 10-20 eggs, but can lay more or as few as 1. Most of the egg masses have a waxy coating of brocosomes around the eggs for protection. The nymphs (5 instars) do not have wings, but develop wing pads in the 5th instar and are generally smaller than the adults, ranging in size from .07 inches (2 mm) to nearly ½ inch (13mm) long. The nymphs have very distinct red eyes. The Sharpshooter can consume about 300 times its own weight in fluids from the xylem vessels of the plants upon which it feeds, thus producing copious amounts of excreta fluid.,This dataset presents the Homalodisca vitripennis genome v1.0. This assembly version is the pre-release version, prior to filtering and quality control by the National Center for Biotechnology Information's GenBank resource (https://www.ncbi.nlm.nih.gov/assembly/GCA_000696855.1). Assembly method details will be available in a forthcoming publication.,NOTE: This gene set is an unstable pre-release (v0.5.3), and was provided to facilitate manual curation and analyses before the official gene set is released. Gene identifiers from this gene set will likely not be maintained.,If you wish to use this dataset, please follow the Baylor College of Medicine's conditions for data use: https://www.hgsc.bcm.edu/bcm-hgsc-conditions-use,

,Manual annotation at the i5k Workspace@NAL (https://i5k.nal.usda.gov) is the review and improvement of gene models derived from computational gene prediction. Community curators compare an existing gene model to evidence such as RNA-Seq or protein alignments from the same or closely related species and modify the structure or function of the gene accordingly, typically following the i5k Workspace@NAL manual annotation guidelines (https://i5k.nal.usda.gov/content/rules-web-apollo-annotation-i5k-pilot-project). If a gene model is missing, the annotator can also use this evidence to create a new gene model. Because manual annotation, by definition, improves or creates gene models where computational methods have failed, it can be a powerful tool to improve computational gene sets, which often serve as foundational datasets to facilitate research on a species.,Here, community curators used manual annotation at the i5k Workspace@NAL to improve computational gene predictions from the dataset Agrilus planipennis genome annotations v0.5.3. The i5k Workspace@NAL set up the Apollo v1 manual annotation software and multiple evidence tracks to facilitate manual annotation. From 2014-10-20 to 2018-07-12, five community curators updated 263 genes, including developmental genes; cytochrome P450s; cathepsin peptidases; cuticle proteins; glycoside hydrolases; and polysaccharide lyases. For this dataset, we used the program LiftOff v1.6.3 to map the manual annotations to the genome assembly GCF_000699045.2. We computed overlaps with annotations from the RefSeq database using gff3_merge from the GFF3toolkit software v2.1.0. FASTA sequences were generated using gff3_to_fasta from the same toolkit. These improvements should facilitate continued research on Agrilus planipennis, or emerald ash borer (EAB), which is an invasive insect pest.,While these manual annotations will not be integrated with other computational gene sets, they are available to view at the i5k Workspace@NAL (https://i5k.nal.usda.gov) to enhance future research on Agrilus planipennis.,

,The data in this repository is to provide the scripts used in the assembly of the Anastrepha ludens genome. It can be used to replicate the steps necessary to assemble the genome described in National Center for Biotechnology Information (NCBI) BioProject: PRJNA803324. This genome assembly workflow was performed on the United States Department of Agriculture (USDA) - SCINet/Mississippi State University High Performance Computing Cluster (HPC) Atlas and installed via Conda.,,

,The Oncopeltus fasciatus genome was recently sequenced and annotated as part of the i5k pilot project by the Baylor College of Medicine. The O. fasciatus research community has manually reviewed and curated the computational gene predictions and generated an official gene set, OGSv1.1.,Oncopeltus fasciatus has been an established lab organism for over 60 years, and has been used for a wide range of studies from physiology to development and evolution. As a relatively conservative and generalized species, it affords a baseline against which other species can be compared.,For example, this species has the same piercing and sucking type mouthparts as its less benign relatives, including the blood-sucking kissing bug, Rhodnius prolixus, and the brown marmorated stink bug, Halyomorpha halys, which are disease vector and agricultural pest species, respectively. Unlike the pest species, the benign, seed-feeding Oncopeltus can be functionally investigated in the lab by RNA interference (RNAi). Comparing the genomes, and conducting experimental lab work in Oncopeltus, will help to identify unique features of the pest species, and thus inform management strategies for them.,More generally, Oncopeltus is a key species for comparisons across the insects. It is one of the few experimentally tractable hemimetabolous species that can ground comparisons with the completely metamorphosing species of the Holometabola (e.g., flies, beetles, wasps). Topics investigated in this framework include reproductive biology and development of the legs, wings, body segments, extraembryonic membranes, and overall establishment of the body plan.,This dataset presents the Oncopeltus fasciatus gene set BCM_v_0.5.3, which was generated computationally. RNA-Seq data was used with additional protein homology data for a MAKER automated annotation of the Oncopeltus fasciatus genome assembly 1.0. Further annotation method details will be available in a forthcoming publication.,NOTE: This gene set is an unstable pre-release (v0.5.3), and was provided to facilitate manual curation and analyses before the official gene set is released. Gene identifiers from this gene set will likely not be maintained.,If you wish to use this dataset, please follow the Baylor College of Medicine's conditions for data use: https://www.hgsc.bcm.edu/bcm-hgsc-conditions-use,

Data were collected on the seed production and genetic diversity of Lepidium draba, an invasive plant. Site characteristics were also measured for 26 sites throughout northern Colorado. In addition, a pollen supplementation experiment was conducted at six of those sites, in which L. draba plants were pollinated with within-population pollen, among-population pollen, or were not pollinated, and seed production was compared among treatments.

The Melampsora lini genome was sequenced to improve its genome assembly. PacBio HiFi and Hi-C data was generated as well as RNA-seq data.

These data contain the results of ʻōhiʻa seedlings challenged with different ambrosia beetle (Coleoptera: Curculionidae) species that were exposed to either Ceratocystis lukuohia or Ceratocystis huliohia in culture. Disease development, viability, and DNA confirmation data were recorded.

,The pecan weevil, Curculio caryae (Horn), is an obligate feeder of pecan and native hickory throughout North America. Subsequently it is a significant agricultural pest in commercial pecan orchards. In this study, we present a reference quality genome using deep-coverage, ~50x PacBio HiFi genome sequence reads, and chromatic confirmation, Hi-C, scaffolding. The final genome assembly is approximately 2.1 Gb, which was confirmed with flow cytometry. The primary genome scaffolds have an N50 of 132 Mb and a BUSCO completeness of 99.1% [S:97.5%, D:1.6%]. Furthermore, we employed PacBio long-read RNA, Iso-seq, for de novo annotation, followed by InterProscan to identify approximately 16,000 protein coding genes. Repeat content is extensive, contributing to >75% of the total genome. This data set provides a valuable resource for use in comparative genomics and evolutionary studies for an economically impactful group of insect pests that currently lack genomic resources.,