Culture presence/absence of M. avium and M. intracellulare by sample qPCR M. avium and M. intracellular detection and quatification by sample. This dataset is associated with the following publication: Pfaller, S., D. King, J. Mistry, and M. Donohue. Occurrence Revisited - Mycobacterium avium and Mycobacterium intracellulare in Potable Water in the USA. Applied Microbiology and Biotechnology. Springer, New York, NY, USA, 106: 2715–2727, (2022).

This dataset contains colony forming unit and qPCR data. This dataset is associated with the following publication: Morris, B., H. Buse, N. Adcock, and E. Rice. A novel broth medium for enhanced growth of Francisella tularensis. Letters in Applied Microbiology. Blackwell Publishing, Malden, MA, USA, 64(6): 393-468, (2017).

This dataset contains colony forming unit and qPCR data. This dataset is associated with the following publication: Morris, B., H. Buse, N. Adcock, and E. Rice. A novel broth medium for enhanced growth of Francisella tularensis. Letters in Applied Microbiology. Blackwell Publishing, Malden, MA, USA, 64(6): 393-468, (2017).

Background Since Mycobacterium avium subspecies paratuberculosis (MAP) was isolated from intestinal tissue of a human patient suffering Crohn's disease, a controversial discussion exists whether MAP have a role in the etiology of Crohn's disease or not. Raw milk may be a potential vehicle for the transmission of MAP to human population. In a previous paper, we have demonstrated that MAP are found in raw milk samples obtained from a defined region in Switzerland. The aim of this work is to collect data about the prevalence of MAP specific IS900 insertion sequence in bulk-tank milk samples in different regions of Switzerland. Furthermore, we examined eventual correlation between the presence of MAP and the somatic cell counts, the total colony counts and the presence of Enterobacteriaceae. Results 273 (19.7%) of the 1384 examined bulk-tank milk samples tested IS900 PCR-positive. The prevalence, however, in the different regions of Switzerland shows significant differences and ranged from 1.7% to 49.2%. Furthermore, there were no statistically significant (p >> 0.05) differences between the somatic cell counts and the total colony counts of PCR-positive and PCR-negative milk samples. Enterobacteriaceae occur as often in IS900 PCR-positive as in PCR-negative milk samples. Conclusion This is the first study, which investigates the prevalence of MAP in bulk-tank milk samples all over Switzerland and infers the herd-level prevalence of MAP infection in dairy herds. The prevalence of 19.7% IS900 PCR-positive bulk-milk samples shows a wide distribution of subclinical MAP-infections in dairy stock in Switzerland. MAP can therefore often be transmitted to humans by raw milk consumption.

The dataset contains the first draft genome sequence of the type strain of Mycobacterium chimaera, Fl-0169. This dataset is associated with the following publication: Pfaller, S., V. Tokarev, C. Kessler, C. McLimans, V. Gomez-Alvarez, J. Wright, D. King, and R. Lamendella. Draft Genome Sequence of Mycobacterium chimaera Type Strain Fl-0169. Genome Announcements. American Society for Microbiology, Washington, DC, USA, 5(8): e01620-16, (2017).

Co-infection data in the form of colony forming units and amoeba cell counts. This dataset is associated with the following publication: Buse , H., F. Schaefer, and G. Rice. Enhanced survival but not amplification of Francisella spp. in the presence of free-living amoebae. Acta Microbiologica et Immunologica Hungarica. Akademiai Kiado, Budapest, HUNGARY, 64(1): 17-36, (2016).