From 2 April - 29 April 2022, a Virginia Tech team of 2-3 sampled the macroinvertebrate community in 30 Coastal Plain streams (Delaware, Maryland and Virginia, USA) as part of a larger stream-health study including other teams who surveyed geomorphology, water quality, flow, temperature,fish community, and fish health at the same 30 streams. Macroinvertebrates were sampled using a 250 micrometer mesh slack sampler on a known area. At each stream, samples were collected in two ways. 1) Five slack samples were taken in riffles according to the USGS National Water-Quality Assessment protocol, and 2) Five additional slack samples were collected in the most productive available habitat types such as woody debris, undercut banks or leaf packs. Additionally, water quality and habitat measurements were collected including water temperature, electrical conductivity, and clarity, and percent of the reach comprising pool, riffle, and run habitats.

From 12 May – 18 May 2023, a Virginia Tech team of 2-3 sampled the macroinvertebrate community in 30 Upper Northern Piedmont streams (Maryland and Pennsylvania, USA) as part of a larger stream-health study including other teams who surveyed geomorphology, water quality, flow, temperature, fish community, and fish health at the same 30 streams. Macroinvertebrates were sampled using a 500-micrometer mesh d-frame net on a known area (0.25 m2). Five d-frame net samples were collected in the most productive available habitat types for that 100-meter stream reach, such as woody debris, undercut banks or leaf packs. Macroinvertebrate data are related to the Chesapeake Bay Index of Biotic Integrity metrics and scores calculated for the Upper North Piedmont region. These macroinvertebrate community data were collected as part of a larger interdisciplinary Chesapeake Stream Team field campaign

From 31 May to 29 June, 2022, a Virginia Tech team of 4-5 sampled the fish community in 30 Delmarva streams (Maryland and Delaware, USA) as part of a larger stream-health study including other teams who surveyed geomorphology, water quality, flow, temperature, macroinvertebrates, and fish health at the same 30 streams. These 30 Chesapeake Bay Watershed tributaries had upstream drainage areas of 9 to 54 sq. km. At each stream, we sampled fish from two reaches using two-pass backpack electrofishing and seining. Reach A was the main reach surveyed by all the interdisciplinary teams; Reach B was surveyed for fish communities only. Reach length was 20 wetted channel widths, capped at 150 meters. Fish were identified to species, assigned to a length class, inspected for anomalies, and released. Other measurements included water temperature, electrical conductivity, and clarity, and streambed area comprising pool, riffle, and run habitats.

From 31 May to 29 June, 2022, a Virginia Tech team of 4-5 sampled the fish community in 30 Delmarva streams (Maryland and Delaware, USA) as part of a larger stream-health study including other teams who surveyed geomorphology, water quality, flow, temperature, macroinvertebrates, and fish health at the same 30 streams. These 30 Chesapeake Bay Watershed tributaries had upstream drainage areas of 9 to 54 sq. km. At each stream, we sampled fish from two reaches using two-pass backpack electrofishing and seining. Reach A was the main reach surveyed by all the interdisciplinary teams; Reach B was surveyed for fish communities only. Reach length was 20 wetted channel widths, capped at 150 meters. Fish were identified to species, assigned to a length class, inspected for anomalies, and released. Other measurements included water temperature, electrical conductivity, and clarity, and streambed area comprising pool, riffle, and run habitats.

From 20 May - 8 June 2021, a Virginia Tech team of 2-3 sampled the macroinvertebrate community in 30 Shenandoah Valley streams (Virginia and West Virginia, USA) as part of a larger stream-health study including other teams who surveyed geomorphology, water quality, flow, temperature,fish community, and fish health at the same 30 streams. The macroinvertebrate community team also characterized the habitat units sampled for macroinvertebrates. At each stream, we sampled macroinvertebrates in two ways:1) in each stream we took 5 slack samples in riffles according to NAWQA protocol, and 2) we selected the most available and productive habitat where we used the Slack sampler to collect 5 additional samples in wood debris accumulations, undercut banks or leaf packs. Mesh size was 250 micrometer and samples were all taken for a known area. Other measurements included water temperature, electrical conductivity, and clarity, and percent of the reach comprising pool, riffle, and run habitats.

From 20 May - 8 June 2021, a Virginia Tech team of 2-3 sampled the macroinvertebrate community in 30 Shenandoah Valley streams (Virginia and West Virginia, USA) as part of a larger stream-health study including other teams who surveyed geomorphology, water quality, flow, temperature,fish community, and fish health at the same 30 streams. The macroinvertebrate community team also characterized the habitat units sampled for macroinvertebrates. At each stream, we sampled macroinvertebrates in two ways:1) in each stream we took 5 slack samples in riffles according to NAWQA protocol, and 2) we selected the most available and productive habitat where we used the Slack sampler to collect 5 additional samples in wood debris accumulations, undercut banks or leaf packs. Mesh size was 250 micrometer and samples were all taken for a known area. Other measurements included water temperature, electrical conductivity, and clarity, and percent of the reach comprising pool, riffle, and run habitats.

From May 27 to June 21, 2024, a Virginia Tech team of 5 sampled the fish community in 30 streams spanning a gradient of the developed landscape, including Potomac River, Rappahannock River, and upper Chesapeake Bay tributaries of Maryland, Washington, D.C., and Virginia, USA. These fish surveys were part of a larger stream-health study including other teams who surveyed geomorphology, water quality, flow, temperature, and macroinvertebrates at the same 30 streams. Upstream drainage area of these 30 streams ranged from approximately 7 to 32 sq. km, and width from 2 to 10 m. At each stream, we sampled fish from two reaches using two-pass backpack electrofishing and seining. Reach A was the main reach surveyed by all the interdisciplinary teams; Reach B was surveyed for fish communities only. Reach length was 20 wetted channel widths, capped at 150 meters. Fish were identified to species, assigned to a length class, inspected for anomalies, and released. Other measurements included water temperature, electrical conductivity, and clarity.

From May 27 to June 21, 2024, a Virginia Tech team of 5 sampled the fish community in 30 streams spanning a gradient of the developed landscape, including Potomac River, Rappahannock River, and upper Chesapeake Bay tributaries of Maryland, Washington, D.C., and Virginia, USA. These fish surveys were part of a larger stream-health study including other teams who surveyed geomorphology, water quality, flow, temperature, and macroinvertebrates at the same 30 streams. Upstream drainage area of these 30 streams ranged from approximately 7 to 32 sq. km, and width from 2 to 10 m. At each stream, we sampled fish from two reaches using two-pass backpack electrofishing and seining. Reach A was the main reach surveyed by all the interdisciplinary teams; Reach B was surveyed for fish communities only. Reach length was 20 wetted channel widths, capped at 150 meters. Fish were identified to species, assigned to a length class, inspected for anomalies, and released. Other measurements included water temperature, electrical conductivity, and clarity.

From 2-27 June, 2023, a Virginia Tech team of 5 sampled the fish community in 30 Piedmont streams (lower Susquehanna River and upper Chesapeake Bay tributaries, Pennsylvania and Maryland, USA) spanning a gradient of agricultural intensity as part of a larger stream-health study including other teams who surveyed geomorphology, water quality, flow, temperature, and macroinvertebrates at the same 30 streams. Upstream drainage area of these 30 streams ranged from approximately 10 to 50 sq. km, and width from 2 to 10 m. At each stream, we sampled fish from two reaches using two-pass backpack electrofishing and seining. Reach A was the main reach surveyed by all the interdisciplinary teams; Reach B was surveyed for fish communities only. Reach length was 20 wetted channel widths, capped at 150 meters. Fish were identified to species, assigned to a length class, inspected for anomalies, and released. Other measurements included water temperature, electrical conductivity, pH, dissolved oxygen, and clarity, and streambed area comprising pool, riffle, and run habitats.

From 2-27 June, 2023, a Virginia Tech team of 5 sampled the fish community in 30 Piedmont streams (lower Susquehanna River and upper Chesapeake Bay tributaries, Pennsylvania and Maryland, USA) spanning a gradient of agricultural intensity as part of a larger stream-health study including other teams who surveyed geomorphology, water quality, flow, temperature, and macroinvertebrates at the same 30 streams. Upstream drainage area of these 30 streams ranged from approximately 10 to 50 sq. km, and width from 2 to 10 m. At each stream, we sampled fish from two reaches using two-pass backpack electrofishing and seining. Reach A was the main reach surveyed by all the interdisciplinary teams; Reach B was surveyed for fish communities only. Reach length was 20 wetted channel widths, capped at 150 meters. Fish were identified to species, assigned to a length class, inspected for anomalies, and released. Other measurements included water temperature, electrical conductivity, pH, dissolved oxygen, and clarity, and streambed area comprising pool, riffle, and run habitats.