From 20 May - 8 June 2021, a Virginia Tech team of 2-3 sampled the macroinvertebrate community in 30 Shenandoah Valley streams (Virginia and West Virginia, USA) as part of a larger stream-health study including other teams who surveyed geomorphology, water quality, flow, temperature,fish community, and fish health at the same 30 streams. The macroinvertebrate community team also characterized the habitat units sampled for macroinvertebrates. At each stream, we sampled macroinvertebrates in two ways:1) in each stream we took 5 slack samples in riffles according to NAWQA protocol, and 2) we selected the most available and productive habitat where we used the Slack sampler to collect 5 additional samples in wood debris accumulations, undercut banks or leaf packs. Mesh size was 250 micrometer and samples were all taken for a known area. Other measurements included water temperature, electrical conductivity, and clarity, and percent of the reach comprising pool, riffle, and run habitats.

From 16 June to 30 July, 2021, a Virginia Tech team of 4-6 sampled the fish community in 30 Shenandoah Valley streams (Virginia and West Virginia, USA) as part of a larger stream-health study including other teams who surveyed geomorphology, water quality, flow, temperature, macroinvertebrates, and fish health at the same 30 streams. The fish community team also sampled three additional bonus streams (SiteID's FOLL, NAKE, and TUMB) during 19-22 August, 2021. These 33 streams had upstream drainage areas of 7 to 46 sq. km and included 4 James River tributaries (SiteID's POAG, MLRB, CEDR, PISG), 1 Potomac River tributary (HARL), and 28 Shenandoah River tributaries. At each stream, we sampled fish from two reaches using two-pass backpack electrofishing and seining. Reach A was the main reach surveyed by all the interdisciplinary teams; Reach B was surveyed for fish communities only. Reach length was 20 wetted channel widths, capped at 150 meters. Fish were identified to species, assigned to a length class, inspected for anomalies, and released. Other measurements included water temperature, electrical conductivity, and clarity, and percent of the reach comprising pool, riffle, and run habitats.

From 16 June to 30 July, 2021, a Virginia Tech team of 4-6 sampled the fish community in 30 Shenandoah Valley streams (Virginia and West Virginia, USA) as part of a larger stream-health study including other teams who surveyed geomorphology, water quality, flow, temperature, macroinvertebrates, and fish health at the same 30 streams. The fish community team also sampled three additional bonus streams (SiteID's FOLL, NAKE, and TUMB) during 19-22 August, 2021. These 33 streams had upstream drainage areas of 7 to 46 sq. km and included 4 James River tributaries (SiteID's POAG, MLRB, CEDR, PISG), 1 Potomac River tributary (HARL), and 28 Shenandoah River tributaries. At each stream, we sampled fish from two reaches using two-pass backpack electrofishing and seining. Reach A was the main reach surveyed by all the interdisciplinary teams; Reach B was surveyed for fish communities only. Reach length was 20 wetted channel widths, capped at 150 meters. Fish were identified to species, assigned to a length class, inspected for anomalies, and released. Other measurements included water temperature, electrical conductivity, and clarity, and percent of the reach comprising pool, riffle, and run habitats.

From 2 April - 29 April 2022, a Virginia Tech team of 2-3 sampled the macroinvertebrate community in 30 Coastal Plain streams (Delaware, Maryland and Virginia, USA) as part of a larger stream-health study including other teams who surveyed geomorphology, water quality, flow, temperature,fish community, and fish health at the same 30 streams. Macroinvertebrates were sampled using a 250 micrometer mesh slack sampler on a known area. At each stream, samples were collected in two ways. 1) Five slack samples were taken in riffles according to the USGS National Water-Quality Assessment protocol, and 2) Five additional slack samples were collected in the most productive available habitat types such as woody debris, undercut banks or leaf packs. Additionally, water quality and habitat measurements were collected including water temperature, electrical conductivity, and clarity, and percent of the reach comprising pool, riffle, and run habitats.

From 2 April - 29 April 2022, a Virginia Tech team of 2-3 sampled the macroinvertebrate community in 30 Coastal Plain streams (Delaware, Maryland and Virginia, USA) as part of a larger stream-health study including other teams who surveyed geomorphology, water quality, flow, temperature,fish community, and fish health at the same 30 streams. Macroinvertebrates were sampled using a 250 micrometer mesh slack sampler on a known area. At each stream, samples were collected in two ways. 1) Five slack samples were taken in riffles according to the USGS National Water-Quality Assessment protocol, and 2) Five additional slack samples were collected in the most productive available habitat types such as woody debris, undercut banks or leaf packs. Additionally, water quality and habitat measurements were collected including water temperature, electrical conductivity, and clarity, and percent of the reach comprising pool, riffle, and run habitats.

From 12 May – 18 May 2023, a Virginia Tech team of 2-3 sampled the macroinvertebrate community in 30 Upper Northern Piedmont streams (Maryland and Pennsylvania, USA) as part of a larger stream-health study including other teams who surveyed geomorphology, water quality, flow, temperature, fish community, and fish health at the same 30 streams. Macroinvertebrates were sampled using a 500-micrometer mesh d-frame net on a known area (0.25 m2). Five d-frame net samples were collected in the most productive available habitat types for that 100-meter stream reach, such as woody debris, undercut banks or leaf packs. Macroinvertebrate data are related to the Chesapeake Bay Index of Biotic Integrity metrics and scores calculated for the Upper North Piedmont region. These macroinvertebrate community data were collected as part of a larger interdisciplinary Chesapeake Stream Team field campaign

The data represent counts and presence of stream-dwelling aquatic macroinvertebrate communities in nine watersheds within the non-connected stormwater region in Kansas City, Missouri. Samples were collected from 3 stream habitats (coarse substrate in cobble riffles, depositional non-flow habitat in pools, and rootmats along shoreline areas). Macroinvertebrate organisms were enumerated and identified to the lowest practical taxonomic unit.

The data represent counts and presence of stream-dwelling aquatic macroinvertebrate communities in nine watersheds within the non-connected stormwater region in Kansas City, Missouri. Samples were collected from 3 stream habitats (coarse substrate in cobble riffles, depositional non-flow habitat in pools, and rootmats along shoreline areas). Macroinvertebrate organisms were enumerated and identified to the lowest practical taxonomic unit.

The United States Geological Survey Virginia and West Virginia Water Science Center (USGS VA-WV-WSC) and Fairfax County assembled this data release in support of ongoing USGS VA-WV-WSC monitoring and evaluations of stream conditions overtime of two previously restored, urban-suburban streams in Reston, Virginia – Snakeden Branch and The Glade. The aquatic benthic macroinvertebrate, fish, and habitat sampling and surveying were conducted on the same eight, 100-meter stream reaches (four reaches in Snakeden Branch and four reaches in The Glade) by the USGS VA-WV-WSC. Aquatic benthic macroinvertebrate sampling and physical habitat surveys were conducted in both Spring and Fall of 2021 and a fish survey was conducted in the Summer 2021. Data provided are of five general types: 1. Site locations and characteristics for each of the eight sampling and surveying sites within the two monitored streams; 2. Benthic macroinvertebrate identifications and analytical laboratory results; 3. Fairfax County benthic macroinvertebrate Index of Biotic Integrity (IBI) scores and individual metric results; 4. Fish assemblages, and; 5. Physical habitat measurements. The metadata file “Metadata_for_Reston_Ecological_Data.xml” contains important information pertaining to the attributes of each entity of data, field and laboratory methods, and caveats associated with the Fall benthic macroinvertebrate IBI and metric scores. The "README.txt" file contains a description of each file contained in this data release. This database contains the aquatic benthic macroinvertebrate taxonomic identification and abundance data derived from samples collected at each of the eight total reaches within Snakeden Branch and The Glade in Fall and Spring of calendar year 2021. Samples were collected by the USGS VA-WV-WSC using the Fairfax County standard operating procedures (FCSOP) and specimen identification and abundance calculations were performed by a third-party laboratory. Fairfax County benthic macroinvertebrate IBI scores and individual metrics were calculated by Fairfax County following FCSOP. It is important to note for this data release that the IBI scores presented for Fall samples have caveats associated with them and the ratings (e.g. Excellent, Good, Fair, etc.) for the Fall samples have been intentionally excluded. Please read the methods section of the “Metadata_for_Reston_Ecological_Data.xml” file carefully for further explanation. This data release also contains the fish assemblages data, collected during the Summer of 2021, from the eight total reaches in Snakeden Branch and The Glade. Electrofishing surveys were conducted following FCSOP by the USGS VA-WV-WSC to obtain these results. Finally, this data release contains the physical habitat data surveyed in both Fall and Spring of 2021. Multiple habitat metrics were measured throughout each of the eight total reaches on Snakeden Branch and The Glade.

The United States Geological Survey Virginia and West Virginia Water Science Center (USGS VA-WV-WSC) and Fairfax County assembled this data release in support of ongoing USGS VA-WV-WSC monitoring and evaluations of stream conditions overtime of two previously restored, urban-suburban streams in Reston, Virginia – Snakeden Branch and The Glade. The aquatic benthic macroinvertebrate, fish, and habitat sampling and surveying were conducted on the same eight, 100-meter stream reaches (four reaches in Snakeden Branch and four reaches in The Glade) by the USGS VA-WV-WSC. Aquatic benthic macroinvertebrate sampling and physical habitat surveys were conducted in both Spring and Fall of 2021 and a fish survey was conducted in the Summer 2021. Data provided are of five general types: 1. Site locations and characteristics for each of the eight sampling and surveying sites within the two monitored streams; 2. Benthic macroinvertebrate identifications and analytical laboratory results; 3. Fairfax County benthic macroinvertebrate Index of Biotic Integrity (IBI) scores and individual metric results; 4. Fish assemblages, and; 5. Physical habitat measurements. The metadata file “Metadata_for_Reston_Ecological_Data.xml” contains important information pertaining to the attributes of each entity of data, field and laboratory methods, and caveats associated with the Fall benthic macroinvertebrate IBI and metric scores. The "README.txt" file contains a description of each file contained in this data release. This database contains the aquatic benthic macroinvertebrate taxonomic identification and abundance data derived from samples collected at each of the eight total reaches within Snakeden Branch and The Glade in Fall and Spring of calendar year 2021. Samples were collected by the USGS VA-WV-WSC using the Fairfax County standard operating procedures (FCSOP) and specimen identification and abundance calculations were performed by a third-party laboratory. Fairfax County benthic macroinvertebrate IBI scores and individual metrics were calculated by Fairfax County following FCSOP. It is important to note for this data release that the IBI scores presented for Fall samples have caveats associated with them and the ratings (e.g. Excellent, Good, Fair, etc.) for the Fall samples have been intentionally excluded. Please read the methods section of the “Metadata_for_Reston_Ecological_Data.xml” file carefully for further explanation. This data release also contains the fish assemblages data, collected during the Summer of 2021, from the eight total reaches in Snakeden Branch and The Glade. Electrofishing surveys were conducted following FCSOP by the USGS VA-WV-WSC to obtain these results. Finally, this data release contains the physical habitat data surveyed in both Fall and Spring of 2021. Multiple habitat metrics were measured throughout each of the eight total reaches on Snakeden Branch and The Glade.