The dataset contains the raw data for the graphs in the paper. This dataset is associated with the following publication: Smith, M., S. Stuntz, Y. Xing, M. Magnuson, R. Phillips, and W.F. Harper. Functional resilience of activated sludge exposed to Bacillus globigii and bacteriophage MS2. Water and Environment Journal. John Wiley & Sons, Inc., Hoboken, NJ, USA, 35(3): 930-936, (2021).

Because of their ubiquity and resistance to spacecraft decontamination bacterial spores are considered likely potential forward contaminants on robotic missions to Mars. Thus it is important to understand their global responses to long-term exposure to space or Mars environments. As part of the PROTECT experiment spores of B. subtilis 168 were exposed to real space conditions and to simulated martian conditions for 559 days in low Earth orbit mounted on the EXPOSE-E exposure platform outside the European Columbus module on the International Space Station. Upon return spores were germinated total RNA extracted and fluorescently labeled and used to probe a custom Bacillus subtilis microarray to identify genes preferentially activated or repressed relative to ground control spores. Increased transcript levels were detected for a number of stress-related regulons responding to DNA damage (SOS response SP-beta prophage induction) protein damage (CtsR/Clp system) oxidative stress (PerR regulon) and cell envelope stress (SigV regulon). Spores exposed to space demonstrated a much broader and more severe stress response than spores exposed to simulated Mars conditions. The results are discussed in the context of planetary protection for a hypothetical journey of potential forward contaminant spores from Earth to Mars and their subsequent residence on Mars. Two-color microarrays were performed comparing germination of Space-exposed or Mars-exposed vs. ground-control (Earth) spores.

Data corresponding to the figures in the paper. This dataset is associated with the following publication: Xing, Y., A. Ellis, M. Magnuson, and W. Harper. Adsorption of bacteriophage MS2 to colloids: Kinetics and particle interactions. Colloids and Surfaces A: Physicochemical and Engineering Aspects. Elsevier B.V., Amsterdam, NETHERLANDS, 585: 124099, (2020).

Complete dataset for "Efficacy of Triethylene Glycol (Grignard Pure) against Bacteriophage MS2 in the Air and on Surfaces". This dataset is associated with the following publication: Ratliff, K., L. Oudejans, J. Archer, M. Calfee, J. Gilberry, D. Hook, W. Schoppman, R. Yaga, L. Brooks, and S. Ryan. Impact of test methodology on the efficacy of triethylene glycol (Grignard Pure) against bacteriophage MS2. AEROSOL SCIENCE AND TECHNOLOGY. Taylor & Francis, Inc., Philadelphia, PA, USA, 57(12): 1178–1185, (2023).

Dataset for bench-scale project evaluating methods to improve detection and sensitivity of Bacillus anthracis detection in 'difficult' sample matrices (i.e., soil, wet vacuum, stormwater runoff). This dataset is associated with the following publication: Nelson, S., K. Hofacre, S. Shah, E. Silvestri, V. Gallardo, A. Mikelonis, R. James, and M. Calfee. Evaluation of Sample Processing Methods to Improve Detection of Bacillus anthracis in Difficult Sample Matrices. ENVIRONMENTAL MONITORING AND ASSESSMENT. Springer, New York, NY, USA, 194(10): 789, (2022).

Comparing the transcriptional responses of Bacillus subtilis strains WN624 and WN1106 at 5 kPa and 101 kPa. WN1106 is a 5 kPa-evolved strain with increased fitness compared to ancestor-WN624 strain at 5 kPa. This experiment probed the difference in response when the strains are grown at 5 kPa. Two-condition experiment 5 kPa vs. 101 kPa for both strains. And two component condition of WN1106 compared to WN624 at either 5 kPa or 101 kPa. 4 pressure comparisons.

Comparing the transcriptional responses of Bacillus subtilis strains WN624 and WN1106 at 5 kPa and 101 kPa. WN1106 is a 5 kPa-evolved strain with increased fitness compared to ancestor-WN624 strain at 5 kPa. This experiment probed the difference in response when the strains are grown at 5 kPa. Two-condition experiment 5 kPa vs. 101 kPa for both strains. And two component condition of WN1106 compared to WN624 at either 5 kPa or 101 kPa. 4 pressure comparisons.

SRA of spore forming Bacillus strains isolated from various areas of the ISS during Microbial tracking investigation

Excel dataset including reduced data for Evaluation of Water Treatments on Surfaces to Inhibit Bacterial Spore Resuspension manuscript. This dataset is associated with the following publication: Hook, D.A., J. Gilberry, C. Johnson, R. Yaga, and J. Archer. Evaluation of Water Treatments on Surfaces to Inhibit Bacterial Spore Resuspension. Remediation Journal. John Wiley & Sons, Inc., Hoboken, NJ, USA, 34(3): e21779, (2024).