MicroRNA expression of C. elegans in space environment

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Entire microRNA expression were analyzed using the Filgen Array miRNA Caenorhabditis elegans 232 probes (Filgen). In the microRNA expression analysis we compared the expression data between two groups: micro-gravity samples (4 d and 8 d) and controls (4 d and 8 d ground control and 1 G centrifuge onboard ISS).

Expression Data from International C. elegans Experiment 1st (ICE-FIRST)

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The effect of microgravity on gene expression in C.elegans was comprehensively analysed by DNA microarray (Agilent). Overall design: Mix staged C. elegans N2 was exposed microgravity for 10 days.

Expression Data from International C. elegans Experiment 1st (ICE-FIRST)

공공데이터포털

The effect of microgravity on gene expression in C.elegans was comprehensively analysed by DNA microarray (Agilent). Overall design: Mix staged C. elegans N2 was exposed microgravity for 10 days.

Microgravity effect on C. elegans N2/VC (CERISE 8days)

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Microgravity effect on C. elegans gene expression was analysed by whole genome microarray. The worms were cultivated under microgravity for 8 days in the Japanese Module of the International Space Station. The samples of this study were divided three experimental groups: 1. microgravity for 8 days 2. artificial 1G control for 8 days on orbit 3. ground 1G control for 8 days This study was repeated with three biological and two technical replicates.

Microgravity effect on C. elegans N2/VC (CERISE 8days)

공공데이터포털

Microgravity effect on C. elegans gene expression was analysed by whole genome microarray. The worms were cultivated under microgravity for 8 days in the Japanese Module of the International Space Station. The samples of this study were divided three experimental groups: 1. microgravity for 8 days 2. artificial 1G control for 8 days on orbit 3. ground 1G control for 8 days This study was repeated with three biological and two technical replicates.

Microgravity effect on C. elegans N2/VC (CERISE 4 days)

공공데이터포털

Microgravity effect on C. elegans gene expression was analysed by whole genome microarray. The worms were cultivated under microgravity for 4 days in the Japanese Module of the International Space Station. C. elegans N2 was exposed microgravity for 4 days. The worms synchronously were cultivated from L1 larvae to adult. There are two control groups onboard 1G and ground 1G control.

Expression data from C. elegans

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We used microarrays to investigate the effects of microgravity and space radiation on the genome-wide expression of the C. elegans. Three technical replicates of wild type C. elegans (CC1 strain) which exposed to space radiation are analyzed along with ground control.

Expression data from C. elegans

공공데이터포털

We used microarrays to investigate the effects of microgravity and space radiation on the genome-wide expression of the C. elegans. Three technical replicates of wild type C. elegans (CC1 strain) which exposed to space radiation are analyzed along with ground control.

Synergistic effects of microgravity and space radiation (Nimblegen)

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Space radiations and microgravity both could cause DNA damage in cells but the effects of microgravity on DNA damage response to space radiations are still controversial. A mRNA microarray and microRNA microarray in dauer larvae of Caenorhabditis elegans (C. elegans) that endured space xef xac x82ight environment and space radiations environment during 16.5-day Shenzhou-8 space mission were performed. In our study wild type dys-1 mutant and ced-1 mutant strains of C.elegans endured four conditions during shenzhou-8 spaceflight mission including spaceflight static condition(ss) spaceflight 1-g centrifugal condition(sc) ground control condition(gc) and no-transport control. Limited to the quantity of worm samples we performed technical-repeat test but not sample-repeat test.Accordingly 12 mRNA microarrays were performed.

Synergistic effects of microgravity and space radiation (Nimblegen)

공공데이터포털

Space radiations and microgravity both could cause DNA damage in cells but the effects of microgravity on DNA damage response to space radiations are still controversial. A mRNA microarray and microRNA microarray in dauer larvae of Caenorhabditis elegans (C. elegans) that endured spaceflight environment and space radiations environment during 16.5-day Shenzhou-8 space mission were performed. In our study wild type dys-1 mutant and ced-1 mutant strains of C.elegans endured four conditions during shenzhou-8 spaceflight mission including spaceflight static condition(ss) spaceflight 1-g centrifugal condition(sc) ground control condition(gc) and no-transport control. Limited to the quantity of worm samples we performed technical-repeat test but not sample-repeat test.Accordingly 12 mRNA microarrays were performed.