Water resources around the world are contaminated with per- and polyfluoroalkyl substances (PFAS) due to releases from point sources on military installations, fire training centers, and chemical manufacturing sites. Non-point sources have also been identified including wastewater effluent, landfills, and biosolids application. PFAS are a major concern to myriad stakeholders as some are known to bioaccumulate, they have eco-toxicity effects, and they are highly recalcitrant. PFAS are often called “forever chemicals” due to their environmental persistence but many precursor PFAS are transformed in the environment by microbes. Recent work has shown that PFAS can be biologically degraded in laboratory studies, but the microbial populations catalyzing degradation are poorly characterized. We conducted laboratory microcosm experiments to investigate the biotransformation of perfluorohexane sulfonamido propyl amine (PFHxSAm), a predominant precursor in 3M Aqueous Film Forming Foam (3M AFFF), by native microbial populations in upwelling sediment-water slurries from Ashumet Pond, MA. Ashumet Pond is affected by multiple PFAS-contaminated groundwater plumes from Joint Base Cape Cod, MA. The field collected samples were used to construct aerobic microcosms for monitoring PFHxSAm biodegradation. Microbial community composition was analyzed at 4 timepoints using Illumina 16S rRNA gene iTag sequencing and quantitative PCR (qPCR) targeting bacterial 16S rRNA genes. Experiments were performed in triplicate and abiotic controls were included in each experiment. The data release contains the results of Illumina iTag sequencing and qPCR for microbial community characterization. Four tables are provided that describe the experimental bottles, the results of DNA quantification, the results of biomass estimates using qPCR, and the taxonomic data based on sequencing results (provided as a biom file).

Somatic coliphages are alternative indicators of fecal pollution and are attractive surrogate for viral pathogens. Here we report the draft genome sequences of three replicate plaques from a novel myoviridae bacteriophage isolated from raw wastewater. Genomes were similar to felix01virus phage and are predicted to contain up to 159 protein coding genes. This dataset is associated with the following publication: Keely, S., M. Herrmann, A. Korajkic, N. Brinkman, B. McMinn, S. Fout, and E. Villegas. Genome Sequences of Escherichia Bacteriophages Isolated from Raw Wastewater. Microbiology Resource Announcements. American Society for Microbiology, Washington, DC, USA, 8(26): e00135-19, (2019).

Somatic coliphages are alternative indicators of fecal pollution and are attractive surrogate for viral pathogens. Here we report the draft genome sequences of three replicate plaques from a novel myoviridae bacteriophage isolated from raw wastewater. Genomes were similar to felix01virus phage and are predicted to contain up to 159 protein coding genes. This dataset is associated with the following publication: Keely, S., M. Herrmann, A. Korajkic, N. Brinkman, B. McMinn, S. Fout, and E. Villegas. Genome Sequences of Escherichia Bacteriophages Isolated from Raw Wastewater. Microbiology Resource Announcements. American Society for Microbiology, Washington, DC, USA, 8(26): e00135-19, (2019).

These data describe microbiological analyses performed on groundwater samples from domestic drinking water supply collected from 42 groundwater wells in the Central Valley of California. Samples were collected between January 2014 and April 2014 for the Groundwater Ambient Monitoring and Assessment (GAMA) program priority basin assessment of the Madera, Chowchilla, and Kings (MACK) groundwater sub-basins’ shallow aquifers. A total of 75 wells were sampled for the MACK study unit between August 2013 and April 2014. Samples for this dataset were vacuum filtered and plated on MI and mEI agars prior to incubation to promote colony growth. Colonies were tallied by their species into columns for various fecal indicator bacteria (FIBs): total coliforms (TCs), Escherichia coli (E. coli), enterococci. Non-target growths were also counted and tallied. Six additional replicate samples were collected for quality assurance. Of the 579 total FIB colonies detected, 106 were selected for polymerase chain reaction (PCR) analysis with the goal of sequencing their DNA. Selected colonies consisted of both target and non-target growths and were taken from 14 samples collected at 13 different wells. DNA sequencing was successful for 34 of the sampled colonies out of a total of 59 submitted. Results for these analyses were reported in FASTA format with the number of bases and their starting position indicated for each batch.

,This repository contains data supporting the publication, "Detection of viral, bacterial, and protozoan pathogens and microbial source tracking markers in paired large- and small-volume water samples." The dataset comprises qPCR concentrations of microbial targets in paired large volume and small volume samples from field studies and laboratory recovery experiments, as described in the main publication.,"Field studies.csv" contains sample data and target concentrations for three field studies, two in groundwater and one in surface water. In the "Groundwater - Private wells (n = 138)" study, large volume samples taken by dead-end ultrafiltration were compared to small volume grab samples. In the remaining two studies, large volume dead-end ultrafiltration and small volume samples were collected synchronously to remove the effect of spatial/temporal heterogeneity during collection. Only the 15 qPCR assays with detections and common to all studies are included. Geographic information is not included to protect the privacy of study participants.,"Recovery data.csv" contains laboratory data for large volume and small volume method recovery of three microbial targets (a bacterium, a virus, and a protozoan) at varying initial concentrations.,"Storage data.csv" contains laboratory data for microbial target decay during storage prior to processing. Liquid small volume samples and large volume ultrafilters were stored at 4 C to simulate normal sample storage and transport conditions. Concentrations of three microbial targets (a bacterium, a virus, and a protozoan) were determined for storage times from 0 to 96 hours.,Descriptive data for all microbial targets is provided in "Target data.csv". An explanation of variables (with units) for all data files can found in "Key to variables.csv".,

Decomposition data of bacterial and viral fecal indicators in common human pollution types. This dataset is associated with the following publication: Wanjugi, P., M. Sivaganesan, A. Korajkic, C. Kelty, B. McMinn, R. Ulrich, V. Harwood, and O. Shanks. Differential Decomposition of Bacterial and Viral Fecal Indicators in Common Human Pollution Types. WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 105: 591-601, (2016).

Decomposition data of bacterial and viral fecal indicators in common human pollution types. This dataset is associated with the following publication: Wanjugi, P., M. Sivaganesan, A. Korajkic, C. Kelty, B. McMinn, R. Ulrich, V. Harwood, and O. Shanks. Differential Decomposition of Bacterial and Viral Fecal Indicators in Common Human Pollution Types. WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 105: 591-601, (2016).

Groundwater and surface-water samples were collected and analyzed for microbial source tracking markers to identify the primary sources of fecal bacteria at a Lake Michigan beach in Northwestern Indiana.

Geospatial dataset. This dataset is associated with the following publication: Korajkic, A., B. McMinn, M. Herrmann, M. Sivaganesan, C. Kelty, P. Clinton, M. Nash, and O. Shanks. Viral and Bacterial Fecal Indicators in Untreated Wastewater across the Contiguous United States Exhibit Geospatial Trends. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, USA, 86(8): e02967-19, (2020).

Geospatial dataset. This dataset is associated with the following publication: Korajkic, A., B. McMinn, M. Herrmann, M. Sivaganesan, C. Kelty, P. Clinton, M. Nash, and O. Shanks. Viral and Bacterial Fecal Indicators in Untreated Wastewater across the Contiguous United States Exhibit Geospatial Trends. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. American Society for Microbiology, Washington, DC, USA, 86(8): e02967-19, (2020).