This ScienceHub entry was developed for the published paper: Consoer et al., 2016, Toxicokinetics of perfluorooctane sulfonate in rainow trout (Oncorhynchus mykiss), Environ. Toxicol. Chem. 35:717-727. Individual rainbow trout were exposed to PFOS by bolus injection (elimination studies) or by adding PFOS to incoming water (branchial uptake studies). The trout were fitted with indwelling catheters and urinary cannulae to permit periodic collection of blood and urine. Additional sampling was conducted to evaluate PFOS uptake from and elimination to respired water. Data obtained from each fish was evaluated using a clearance-volume pharmacokinetic model. Modeled kinetic parameters were then averaged to develop summary statistics which were used as a basis for interpreting modeled results and making comparisons to a previous study of rainbow trout exposed to perfluorooctanoate (PFOA; Consoer et al., 2014, Aquat. Toxicol. 156:65-73). The results of this study, combined with that of the previous PFOA study, suggest that PFOA is a substrate for renal transporters in fish while glomerular filtration alone may be sufficient to explain the observed renal elimination of PFOS. These findings demonstrate that models developed to predict the bioaccumulation of perfluoroalkyl acids by fish must account for differences in renal clearance of individual compounds. This dataset is associated with the following publication: Consoer, D., A. Hoffman , P. Fitzsimmons , P. Kosian , and J. Nichols. Toxicokinetics of perfluorooctane sulfonate in rainbow trout (Oncorhynchus mykiss). ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 35(3): 717-727, (2016).

Figure S1: BMC curves. Table S1: Fish assessments. Table S2: Data to make visualization of toxicity (Figure 3). Table S3: Data to make BMC curves. This dataset is associated with the following publication: Britton, K., R. Judson, B. Hill, K. Jarema, J. Olin, B. Knapp, M. Lowery, M. Feshuk, J. Brown, and S. Padilla. Using Zebrafish to Screen Developmental Toxicity of Per- and Polyfluoroalkyl Substances (PFAS). Toxics. MDPI, Basel, SWITZERLAND, 12(7): 501, (2024).

Data for "Estrogenic Activity of Perfluoro Carboxylic and Sulfonic Acids in Rainbow Trout Estrogen Receptor Binding and Liver Slice Vtg mRNA Expression Assays. Mark A. Tapper, Jeffrey S. Denny, Barbara R. Sheedy, Ben Johnson, and Richard C. Kolanczyk. Applied In Vitro Toxicology 2023 9:1, 13-22". This dataset is associated with the following publication: Tapper, M., J. Denny, B. Sheedy, B. Johnson, and R. Kolanczyk. Estrogenic Activity of Perfluoro Carboxylic and Sulfonic Acids in Rainbow Trout Estrogen Receptor Binding and Liver Slice Vtg mRNA Expression Assays. Applied In Vitro Toxicology. Mary Ann Liebert, Inc., Larchmont, NY, USA, 9(1): 13-22, (2023).

Data for "Estrogenic Activity of Perfluoro Carboxylic and Sulfonic Acids in Rainbow Trout Estrogen Receptor Binding and Liver Slice Vtg mRNA Expression Assays. Mark A. Tapper, Jeffrey S. Denny, Barbara R. Sheedy, Ben Johnson, and Richard C. Kolanczyk. Applied In Vitro Toxicology 2023 9:1, 13-22". This dataset is associated with the following publication: Tapper, M., J. Denny, B. Sheedy, B. Johnson, and R. Kolanczyk. Estrogenic Activity of Perfluoro Carboxylic and Sulfonic Acids in Rainbow Trout Estrogen Receptor Binding and Liver Slice Vtg mRNA Expression Assays. Applied In Vitro Toxicology. Mary Ann Liebert, Inc., Larchmont, NY, USA, 9(1): 13-22, (2023).

Supplementary materials for "Non-Targeted Analysis (NTA) of Plasma and Liver from Sprague Dawley Rats Exposed to Perfluorohexanesulfonamide (PFHxSA), a Precursor to Perfluorohexane Sulfonic Acid (PFHxS)"

This dataset describes the results of a set of experiments, the goal of which was to evaluate the effects of protease inhibitors on the trout liver S9 substrate depletion assay. The presented data show that addition of phenylmethylsulfonyl fluoride (PMSF), a serine protease inhibitor, substantially increases the working lifetime of the trout S9 assay, resulting in improved detection of low intrinsic clearance rates. These findings substantially increase the utility of the trout S9 assay and may have broad implications for its use to support chemical bioaccumulation assessments. This dataset is associated with the following publication: Nichols, J., A. Hoffman, J. Swintek, S. Droge, and P. Fitzsimmons. Addition of phenylmethylsulfonyl fluoride (PMSF) substantially increases the working lifetime of the trout liver S9 substrate depletion assay resulting in improved detection of low intrinsic clearance rates. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 40(1): 148-161, (2021).

This dataset describes the results of a set of experiments, the goal of which was to evaluate the effects of protease inhibitors on the trout liver S9 substrate depletion assay. The presented data show that addition of phenylmethylsulfonyl fluoride (PMSF), a serine protease inhibitor, substantially increases the working lifetime of the trout S9 assay, resulting in improved detection of low intrinsic clearance rates. These findings substantially increase the utility of the trout S9 assay and may have broad implications for its use to support chemical bioaccumulation assessments. This dataset is associated with the following publication: Nichols, J., A. Hoffman, J. Swintek, S. Droge, and P. Fitzsimmons. Addition of phenylmethylsulfonyl fluoride (PMSF) substantially increases the working lifetime of the trout liver S9 substrate depletion assay resulting in improved detection of low intrinsic clearance rates. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 40(1): 148-161, (2021).

Our findings identified five structural categories of PFAS prone to metabolism. The metabolism pathways of PFAS are highly related to their structures and significantly impact their bioaccumulations and toxicities. We also verified CES1 as an enzyme capable of hydrolyzing diverse PFAS, with strong substrate preferences towards perfluoroalkyl amides. This dataset is not publicly accessible because: Data generated by external academic lab with chemicals provided by EPA under an MTA. EPA’s contribution was providing the PFAS library. It can be accessed through the following means: Contact the corresponding author Hui Peng, Mailing address: Department of Chemistry, University of Toronto, Toronto, Ontario, M5S3H6, Canada. E-mail address: hui.peng@utoronto.ca. Format: Not available. This dataset is associated with the following publication: Han, J., W. Gu, H. Barrett, D. Yang, S. Tang, J. Sun, J. Liu, H. Krause, K. Houck, and H. Peng. A Roadmap to the Structure-Related Metabolism Pathways of Per- and Polyfluoroalkyl Substances in the Early Life Stages of Zebrafish (Danio rerio). ENVIRONMENTAL HEALTH PERSPECTIVES. National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, NC, USA, 129(7): 077004, (2021).