RAW DATA, SAS FILES AND DATA MEANS. This dataset is associated with the following publication: Howdeshell, K., C. Rider, V. Wilson , J. Furr , C. Lambright , and E. Gray. Dose addition models based on biologically-relevant reductions in fetal testosterone accurately predict postnatal reproductive tract alterations by a phthalate mixture in rats. TOXICOLOGICAL SCIENCES. Society of Toxicology, 148(2): 488-502, (2015).

Data are summarized in a two-dimensional data matrix that was developed for each substance for hazard characterization (Tables S1–S3). In the horizontal direction of the matrix, read-across of the target phenol to the source analogues was performed for the purpose of data-gap filling, whereas in the vertical direction, data from different streams (traditional and NAM) were compared and contrasted, to evaluate concordance of orthogonal approaches for evaluating potential estrogenicity. The greater the degree of agreement in orthogonal approaches for determining bioactivity, the greater the confidence one has in using the collective results of such NAMs in hazard characterization of the target phenol. This dataset is associated with the following publication: Webster, F., M. Gagne, G. Patlewicz, P. Pradeep, N. Trefiak, R. Judson, and T. Barton-Maclaren. Predicting estrogen receptor activation by a group of substituted phenols: An integrated approach to testing and assessment case study. REGULATORY TOXICOLOGY AND PHARMACOLOGY. Elsevier Science Ltd, New York, NY, USA, 106: 278-291, (2019).

Liquid chromatography tandem mass spectrometry allows for the measurement of steroid hormone suites in the blubber of marine mammals. By combining this technology with remote biopsy collection, endocrine profiles can be assessed, allowing for studies of hormonal profile variation over time. In this study, we explored associations among different steroidogenic pathways and seasonal differences in blubber hormone profiles of free-ranging common bottlenose dolphins along the coast of South Carolina, USA. We found that male dolphins experience a peak in testosterone, androstenedione, progesterone, and 17-hydroxyprogesterone in the spring, likely related to an upregulation of the androgen steroidogenic pathway during mating season. We also observed increased cortisol concentrations not related to blubber lipid percentages during summer compared to other months. Though the sample size of females was insufficient to describe female seasonal patterns, there was an increase in androstenedione among females with elevated progesterone concentrations indicative of pregnancy, highlighting another potential endocrine marker for pregnancy in free-ranging dolphins. This work emphasizes the importance of selecting the appropriate season for studies on endocrine status to effectively uncover physiological variation or disruption in free-ranging cetaceans.

metadata sheet, data sheet for each table and figure in the published manuscript. This dataset is associated with the following publication: Gray , E., J. Furr , K. Tatum-Gibbs, C. Lambright , H. Sampson, B. Hannas, V. Wilson , A. Hotchkiss , and P. Foster. Establishing the Biological Relevance of Dipentyl Phthalate Reductions in Fetal Rat Testosterone Production and Plasma and Testis Testosterone Levels. TOXICOLOGICAL SCIENCES. Society of Toxicology, 149(1): 178-91, (2016).

This dataset was generated to support parameterization of a larval amphibian thyroid axis computational model. Specifically, plasma iodine levels were determined not only to inform a concentration value for model parameter specification in the model, but also to support the notion that aquatic and terrestrial species should be considered substantially different with regard to circulating iodide levels. Other computational model physical parameters were informed by evaluating Xenopus laevis thyroid gland histological images for morphological features including thyroid follicular cell numbers and areas. This dataset is associated with the following publication: Haselman, J., J. Nichols, K. Mattingly, M. Hornung, and S. Degitz. A biologically based computational model for the hypothalamic-pituitary-thyroid (HPT) axis in Xenopus laevis larvae. MATHEMATICAL BIOSCIENCES. Elsevier Science Ltd, New York, NY, USA, 362: 109021, (2023).

List of biomarker genes used to predict estrogen receptor activity in MCF-7 cells; list of microarray accession numbers used in the study. This dataset is associated with the following publication: Vanduyn, N., B. Chorley , R. Tice, R. Judson , and C. Corton. Moving Toward Integrating Gene Expression Profiling into High-throughput Testing:A Gene Expression Biomarker Accurately Predicts Estrogen Receptor α Modulation in a Microarray Compendium. TOXICOLOGICAL SCIENCES. Society of Toxicology, 151(1): 88-103, (2016).

Data and supplemental files from COMPARA (A large-scale modeling project). COMPARA combined multiple models developed in collaboration with modelers and computational toxicology scientists from 25 international groups to predict androgen receptor activity of a common set of 55,450 chemical structures. Quantitative structure-activity relationship models and docking approaches were employed to build a total of 91 predictive models for binding, agonist, and antagonist. The consensus values for agonist and antagonist activity are being made public through the CompTox Chemicals Dashboard. This dataset is associated with the following publication: Mansouri, K., N. Kleinstreuer, R. Judson, A. Williams, I. Shah, and A. Richard. CoMPARA: Collaborative Modeling Project for Androgen Receptor Activity. ENVIRONMENTAL HEALTH PERSPECTIVES. National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, NC, USA, 128(2): 27002, (2020).

HT-H295R data was downloaded using the ToxCast pipeline (tcpl) R package and is publicly available. Multi-concentration level 0 data from invitrodb (version 3.1) were downloaded and converted from g/ml into micromolar concentrations prior to calculation of mMds and data simulation (Supplemental Data 1). This dataset is associated with the following publication: Haggard, D., W. Setzer, R. Judson, and K. Friedman. Development of a prioritization method for chemical-mediated effects on steroidogenesis using an integrated statistical analysis of high-throughput H295R data. REGULATORY TOXICOLOGY AND PHARMACOLOGY. Elsevier Science Ltd, New York, NY, USA, 109: 104510, (2019).

File contains data on body, liver, thyroid weights from dams and exposed offspring, serum thyroid hormones, gland and cortical gene expression, results of cognitive function tests. This dataset is associated with the following publication: Ramhoj, L., U. Haas, M. Gilbert, C. Wood, T. Svingen, D. Usai, A.M. Vinggaard, K. Mandrup, and M. Axelstad. Evaluating thyroid hormone disruption: Investigations of long-term neurodevelopmental effects in rats after perinatal exposure to perfluorohexane sulfonate (PFHxS). Scientific Reports. Nature Publishing Group, London, UK, 10(1): 2672, (2020).

Data associated with the Adams et al. manuscript "Elevated winter stream temperatures below wastewater treatment plants shift female reproductive development of Johnny Darter Etheostoma nigrum: a field and histologic approach." Data include in vitro estrogen receptor agonist activities of surface water samples and vitellogenin gene expression in Johnny Darter liver samples. This dataset is associated with the following publication: Adams, C., D. Winkelman, P. Schaffer, R. Fitzpatrick, J. Cavallin, M. Ellman, K. Santana Rodriguez, and D. Villeneuve. Elevated winter stream temperatures below wastewater treatment plants shift reproductive development of Johnny Darter Etheostoma nigrum: a field and histologic approach. Fishes. MDPI, Basel, SWITZERLAND, N/A, (2022).