AIS NL Biofouling Species Fisheries and Oceans Canada's (DFO) National Marine Biofouling Monitoring Program conducts annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and some non-native species in Newfoundland and Labrador (NL) Region since 2006. Standardized monitoring protocols employed by DFO's NL, Maritimes, Gulf, and Quebec regions include biofouling collector plates deployed from May to October at georeferenced intertidal and shallow subtidal sites, including public docks, and public and private marinas and nautical clubs. Initially, (2006-2017), the collectors consisted of three 10 cm by 10 cm PVC plates deployed in a vertical array and spaced approximately 40 cm apart, with the shallowest plate suspended at least 1 m below the surface to sample subtidal and shallow intertidal species (McKenzie et al 2016a). Three replicate arrays were deployed at least 5 m apart per site. Since 2018, collector networks have been modified to improve statistical replication, including up to 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. Since 2006, seven invasive biofouling organisms have been detected in Newfoundland and Labrador harbours, marinas and coastal areas. Should be cited as follows: DFO Newfoundland and Labrador Region Aquatic Invasive Species Marine Biofouling Monitoring Program. Published March 2024. Coastal and Freshwater Ecology, Northwest Atlantic Fisheries Centre, Fisheries and Oceans Canada, St. John’s, Newfoundland and Labrador. Reference: Tunicates Vase tunicate (Ciona intestinalis) 2012 The Vase tunicate, is a high impact solitary invader and was first detected by DFO in 2012 on the Burin Peninsula at Ship Cove and Little Bay, Placentia Bay. Various mitigation measures (McKenzie et al. 2016b) contained this invasive tunicate to a small area for six years within Placentia Bay. First detected in Fortune Bay as an established population in 2019, increasing reports of the Vase tunicate have been made along the south coast of Newfoundland. The data provided here indicates the detections of this AIS in coastal NL. From 2018-2022, the Coastal Environmental Baseline Program provided additional support to enhance sampling efforts in Placentia Bay.

AIS NL Biofouling Species Fisheries and Oceans Canada's (DFO) National Marine Biofouling Monitoring Program conducts annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and some non-native species in Newfoundland and Labrador (NL) Region since 2006. Standardized monitoring protocols employed by DFO's NL, Maritimes, Gulf, and Quebec regions include biofouling collector plates deployed from May to October at georeferenced intertidal and shallow subtidal sites, including public docks, and public and private marinas and nautical clubs. Initially, (2006-2017), the collectors consisted of three 10 cm by 10 cm PVC plates deployed in a vertical array and spaced approximately 40 cm apart, with the shallowest plate suspended at least 1 m below the surface to sample subtidal and shallow intertidal species (McKenzie et al 2016a). Three replicate arrays were deployed at least 5 m apart per site. Since 2018, collector networks have been modified to improve statistical replication, including up to 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. Since 2006, seven invasive biofouling organisms have been detected in Newfoundland and Labrador harbours, marinas and coastal areas. Should be cited as follows: DFO Newfoundland and Labrador Region Aquatic Invasive Species Marine Biofouling Monitoring Program. Published March 2024. Coastal and Freshwater Ecology, Northwest Atlantic Fisheries Centre, Fisheries and Oceans Canada, St. John’s, Newfoundland and Labrador. Reference: Tunicates Violet tunicate (Botrylloides violaceus) 2007 The violet tunicate was first detected in NL waters in 2007 in Belleoram, Fortune Bay on wharf structures and vessels (McKenzie et al. 2016b). This colonial tunicate forms irregular shaped colonies usually of a solid color (orange, purple, yellow or cream). It is currently found in relatively small colonies in four harbours in NL; Placentia Bay (1), Fortune Bay (1), Conception Bay (1) and the west coast of NL (2). The data provided here indicates the detections of this AIS in coastal NL. From 2018-2022, the Coastal Environmental Baseline Program provided additional support to enhance sampling efforts in Placentia Bay.

AIS NL Biofouling Species Fisheries and Oceans Canada's (DFO) National Marine Biofouling Monitoring Program conducts annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and some non-native species in Newfoundland and Labrador (NL) Region since 2006. Standardized monitoring protocols employed by DFO's NL, Maritimes, Gulf, and Quebec regions include biofouling collector plates deployed from May to October at georeferenced intertidal and shallow subtidal sites, including public docks, and public and private marinas and nautical clubs. Initially, (2006-2017), the collectors consisted of three 10 cm by 10 cm PVC plates deployed in a vertical array and spaced approximately 40 cm apart, with the shallowest plate suspended at least 1 m below the surface to sample subtidal and shallow intertidal species (McKenzie et al 2016a). Three replicate arrays were deployed at least 5 m apart per site. Since 2018, collector networks have been modified to improve statistical replication, including up to 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. Since 2006, seven invasive biofouling organisms have been detected in Newfoundland and Labrador harbours, marinas and coastal areas. Should be cited as follows: DFO Newfoundland and Labrador Region Aquatic Invasive Species Marine Biofouling Monitoring Program. Published March 2024. Coastal and Freshwater Ecology, Northwest Atlantic Fisheries Centre, Fisheries and Oceans Canada, St. John’s, Newfoundland and Labrador. Reference: Tunicates Golden star tunicate (Botryllus schlosseri) 2006 The Golden star tunicate was the first invasive tunicate detected in NL waters. It was reported in Argentia by the US Navy around 1945. It was found in 2006 on wharf structures in Argentia, Placentia Bay during the first AIS survey (Callahan et al 2010). This colonial tunicate is recognized by it star shaped grouping of individuals within the colony. It is currently found in Placentia Bay, Fortune Bay, St. Mary’s Bay, Conception Bay and the west coast of NL. The data provided here indicates the detections of this AIS in coastal NL. From 2018-2022, the Coastal Environmental Baseline Program provided additional support to enhance sampling efforts in Placentia Bay.

The Nonindigenous Aquatic Species Database (NAS) information resource is an established central repository for spatially referenced biogeographic accounts of introduced aquatic species. The NAS website provides scientific reports, online/real-time queries, spatial data sets, distribution maps, fact sheets, and general information.

Aquatic invasive species pose economic and ecological threats to Canada's coastal waters. In response, Fisheries and Oceans Canada (DFO) has established monitoring programs to detect and track the spread of aquatic invasive species, including European Green Crab, in Canadian waters. Fukui traps have been deployed annually at both new and long-term monitoring locations throughout coastal British Columbia.

Fisheries and Oceans Canada’s (DFO) National Biofouling Monitoring Program (BMP) has conducted annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and non-indigenous species (NIS) since 2006. Standardized monitoring protocols employed by DFO-Maritimes, -Gulf, and -Quebec Regions include biofouling collector plates deployed from May to October at intertidal and shallow subtidal, geo-referenced sites, including public and private docks, aquaculture lease sites, public and private marinas and yacht clubs. Initially in the Maritimes Region (2006-2017) collectors consisted of 3, 10 cm by 10cm PVC plates deployed in a vertical array and spaced approximately 40-cm apart with the shallowest plate hung at least 1 m below the surface to sample shallow subtidal and intertidal species (Sephton et al. 2011, 2017). Two replicate arrays were deployed at least 5 m apart per site. Since 2018, collector arrays were modified to enhance statistical replication, including 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. The percent cover of AIS on all collectors was determined by visual examination and scored as follows; (i) ‘0’ = absent, (ii) ‘1’ = ≤25 % cover, (iii) ‘2’ = 25 to ≤50 %, (iv) ‘3’ = 50–75% , and (v) ‘4’ = >75%. Average percent cover is provided for all NIS observed annually per site. Presence-absence indicates that an NIS was observed on at least one collector plate. One additional rocky intertidal species (Asian shore crab; Hemigrapsus sanguineus) was assessed via beach surveys as permitted by time and resources following its initial siting in St Mary’s Bay (Nova Scotia) in April 2020. Rapid assessment surveys conducted in the Fall of 2020 and 2021 were employed to delineate H. sanguineus’ distribution and relative abundance. Areas deemed suitable and at high risk for spread were targeted, including exposed rocky intertidal habitat in southwest regions of Nova Scotia and New Brunswick. Each rapid assessment consisted of 30-minute beach surveys per site conducted by 2 or 3 people (modified from Stephenson et al. 2011). During each survey, crabs were collected under rocks and seaweed in preferred cobble/boulder habitat (Lohrer et al. 2000). Count data was standardized for each site as the number of crabs collected per 30-min search per person. Cite as: DFO-Maritimes Biofouling Monitoring Program. Published October 2018, Updated December 2023. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, NS Citations: Sephton D, B Vercaemer, JM Nicolas, J Keays (2011) Monitoring for invasive tunicates in Nova Scotia, Canada (2006-2009) Aquatic Invasions 6: 391-403. Sephton D, B Vercaemer, A Silva, L Stiles, M Harris, K Godin (2017) Biofouling monitoring for aquatic invasive species (AIS) in DFO Maritimes Regions (Atlantic shore of Nova Scotia and southwest New Brunswick): May-November, 2012-2015. Canadian Technical Report of Fisheries and Aquatic Sciences 3158: 72 pp. Stephenson EH, RS Steneck, RH Seeley (2009) Possible temperature limits to range expansion of non-native Asian shore crabs in Maine. Journal of Experimental Marine Biology and Ecology 375: 21–31. doi:10.1016/j.jembe.2009.04.020

The Oceans Act (1997) commits Canada to maintaining biological diversity and productivity in the marine environment. A key component of this is to identify areas that are considered ecologically or biologically significant. Fisheries and Oceans Canada (DFO) Science has developed guidance on the identification of Ecologically or Biologically Significant Areas (EBSAs) (DFO 2004) and has endorsed the scientific criteria of the Convention on Biological Diversity (CBD) for identifying ecologically or biologically significant marine areas as defined in Annex I of Decision IX/20 of its 9th Conference of Parties. These criteria were applied to the Newfoundland and Labrador (NL) Shelves Bioregion in two separate data-driven processes. The first process focused on the area north of the Placentia Bay-Grand Banks (PBGB) Large Ocean Management Area (LOMA) (DFO 2013). The second process focused on the PBGB area (DFO 2019), where EBSAs had previously been identified using a more Delphic approach (Templeman 2007). In both cases, an EBSA Steering Committee, comprised of experts in oceanography, ecosystem structure and function, taxa-specific life histories and Geographic Information Systems (GIS) guided the process by advising or aiding in the identification, collection, processing and analysis of data layers, as well as participating in the final selection of candidate EBSAs (Wells et al. 2017, Ollerhead et al. 2017, Wells et al. 2019). All information was compiled in a GIS and a hierarchical approach was used to review individual data layers and groupings of data layers. Peer review meetings were held for both processes, during which candidate EBSAs were reviewed and the final EBSAs were agreed upon and delineated. In the northern study area, a total of fifteen EBSAs were identified and described; three of these areas are primarily coastal areas; seven are in offshore areas; four EBSAs straddle coastal and offshore areas; and one is a transitory EBSA that follows the southern extent of pack ice. In the PBGB study area, fourteen EBSAs were identified in two different categories: seven based on coastal data and seven based on offshore data. In comparing the new PBGB EBSAs to those identified in 2007, nine of them overlap spatially and are based on similar features; however, there were some variations in the boundaries. Two of the EBSAs that were identified in 2007 were no longer considered EBSAs in 2017, but portions of both of these areas were captured in part by other EBSAs. Five new EBSAs were identified in areas not previously considered. References: DFO, 2004. Identification of Ecologically and Biologically Significant Areas. DFO Can. Sci. Advis. Sec. Ecosystem Status Rep. 2004/006. DFO. 2013. Identification of additional Ecologically and Biologically Significant Areas (EBSAs) within the Newfoundland and Labrador Shelves Bioregion. DFO Can. Sci. Advis. Sec. Sci. Advis. Rep. 2013/048. DFO. 2019. Re-evaluation of the Placentia Bay-Grand Banks Area to Identify Ecologically and Biologically Significant Areas . DFO Can. Sci. Advis. Sec. Sci. Advis. Rep. 2019/040. Ollerhead, L.M.N., Gullage, M., Trip, N., and Wells, N. 2017. Development of Spatially Referenced Data Layers for Use in the Identification and Delineation of Candidate Ecologically and Biologically Significant Areas in the Newfoundland and Labrador Shelves Bioregion. DFO Can. Sci. Advis. Sec. Res. Doc. 2017/036. v + 38 p Templeman, N.D. 2007. Placentia Bay-Grand Banks Large Ocean Management Area Ecologically and Biologically Significant Areas. Can. Sci. Advis. Sec. Res. Doc. 2007/052: iii + 15 p. Wells, N.J., Stenson, G.B., Pepin, P., and Koen-Alonso, M. 2017. Identification and Descriptions of Ecologically and Biologically Significant Areas in the Newfoundland and Labrador Shelves Bioregion. DFO Can. Sci. Advis. Sec. Res. Doc. 2017/013. v + 87 p. Wells, N., K. Tucker, K. Allard, M. Warren, S. Olson, L. Gullage, C. Pretty, V. Sutton-Pande and K. Clarke. 2019. Re-evaluation of the