AIS NL Biofouling Species Fisheries and Oceans Canada's (DFO) National Marine Biofouling Monitoring Program conducts annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and some non-native species in Newfoundland and Labrador (NL) Region since 2006. Standardized monitoring protocols employed by DFO's NL, Maritimes, Gulf, and Quebec regions include biofouling collector plates deployed from May to October at georeferenced intertidal and shallow subtidal sites, including public docks, and public and private marinas and nautical clubs. Initially, (2006-2017), the collectors consisted of three 10 cm by 10 cm PVC plates deployed in a vertical array and spaced approximately 40 cm apart, with the shallowest plate suspended at least 1 m below the surface to sample subtidal and shallow intertidal species (McKenzie et al 2016a). Three replicate arrays were deployed at least 5 m apart per site. Since 2018, collector networks have been modified to improve statistical replication, including up to 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. Since 2006, seven invasive biofouling organisms have been detected in Newfoundland and Labrador harbours, marinas and coastal areas. Should be cited as follows: DFO Newfoundland and Labrador Region Aquatic Invasive Species Marine Biofouling Monitoring Program. Published March 2024. Coastal and Freshwater Ecology, Northwest Atlantic Fisheries Centre, Fisheries and Oceans Canada, St. John’s, Newfoundland and Labrador. Reference: Tunicates Violet tunicate (Botrylloides violaceus) 2007 The violet tunicate was first detected in NL waters in 2007 in Belleoram, Fortune Bay on wharf structures and vessels (McKenzie et al. 2016b). This colonial tunicate forms irregular shaped colonies usually of a solid color (orange, purple, yellow or cream). It is currently found in relatively small colonies in four harbours in NL; Placentia Bay (1), Fortune Bay (1), Conception Bay (1) and the west coast of NL (2). The data provided here indicates the detections of this AIS in coastal NL. From 2018-2022, the Coastal Environmental Baseline Program provided additional support to enhance sampling efforts in Placentia Bay.

AIS NL Biofouling Species Fisheries and Oceans Canada's (DFO) National Marine Biofouling Monitoring Program conducts annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and some non-native species in Newfoundland and Labrador (NL) Region since 2006. Standardized monitoring protocols employed by DFO's NL, Maritimes, Gulf, and Quebec regions include biofouling collector plates deployed from May to October at georeferenced intertidal and shallow subtidal sites, including public docks, and public and private marinas and nautical clubs. Initially, (2006-2017), the collectors consisted of three 10 cm by 10 cm PVC plates deployed in a vertical array and spaced approximately 40 cm apart, with the shallowest plate suspended at least 1 m below the surface to sample subtidal and shallow intertidal species (McKenzie et al 2016a). Three replicate arrays were deployed at least 5 m apart per site. Since 2018, collector networks have been modified to improve statistical replication, including up to 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. Since 2006, seven invasive biofouling organisms have been detected in Newfoundland and Labrador harbours, marinas and coastal areas. Should be cited as follows: DFO Newfoundland and Labrador Region Aquatic Invasive Species Marine Biofouling Monitoring Program. Published March 2024. Coastal and Freshwater Ecology, Northwest Atlantic Fisheries Centre, Fisheries and Oceans Canada, St. John’s, Newfoundland and Labrador. Reference: Tunicates Golden star tunicate (Botryllus schlosseri) 2006 The Golden star tunicate was the first invasive tunicate detected in NL waters. It was reported in Argentia by the US Navy around 1945. It was found in 2006 on wharf structures in Argentia, Placentia Bay during the first AIS survey (Callahan et al 2010). This colonial tunicate is recognized by it star shaped grouping of individuals within the colony. It is currently found in Placentia Bay, Fortune Bay, St. Mary’s Bay, Conception Bay and the west coast of NL. The data provided here indicates the detections of this AIS in coastal NL. From 2018-2022, the Coastal Environmental Baseline Program provided additional support to enhance sampling efforts in Placentia Bay.

Fisheries and Oceans Canada’s (DFO) National Biofouling Monitoring Program (BMP) has conducted annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and non-indigenous species (NIS) since 2006. Standardized monitoring protocols employed by DFO-Maritimes, -Gulf, and -Quebec Regions include biofouling collector plates deployed from May to October at intertidal and shallow subtidal, geo-referenced sites, including public and private docks, aquaculture lease sites, public and private marinas and yacht clubs. Initially in the Maritimes Region (2006-2017) collectors consisted of 3, 10 cm by 10cm PVC plates deployed in a vertical array and spaced approximately 40-cm apart with the shallowest plate hung at least 1 m below the surface to sample shallow subtidal and intertidal species (Sephton et al. 2011, 2017). Two replicate arrays were deployed at least 5 m apart per site. Since 2018, collector arrays were modified to enhance statistical replication, including 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. The percent cover of AIS on all collectors was determined by visual examination and scored as follows; (i) ‘0’ = absent, (ii) ‘1’ = ≤25 % cover, (iii) ‘2’ = 25 to ≤50 %, (iv) ‘3’ = 50–75% , and (v) ‘4’ = >75%. Average percent cover is provided for all NIS observed annually per site. Presence-absence indicates that an NIS was observed on at least one collector plate. One additional rocky intertidal species (Asian shore crab; Hemigrapsus sanguineus) was assessed via beach surveys as permitted by time and resources following its initial siting in St Mary’s Bay (Nova Scotia) in April 2020. Rapid assessment surveys conducted in the Fall of 2020 and 2021 were employed to delineate H. sanguineus’ distribution and relative abundance. Areas deemed suitable and at high risk for spread were targeted, including exposed rocky intertidal habitat in southwest regions of Nova Scotia and New Brunswick. Each rapid assessment consisted of 30-minute beach surveys per site conducted by 2 or 3 people (modified from Stephenson et al. 2011). During each survey, crabs were collected under rocks and seaweed in preferred cobble/boulder habitat (Lohrer et al. 2000). Count data was standardized for each site as the number of crabs collected per 30-min search per person. Cite as: DFO-Maritimes Biofouling Monitoring Program. Published October 2018, Updated December 2023. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, NS Citations: Sephton D, B Vercaemer, JM Nicolas, J Keays (2011) Monitoring for invasive tunicates in Nova Scotia, Canada (2006-2009) Aquatic Invasions 6: 391-403. Sephton D, B Vercaemer, A Silva, L Stiles, M Harris, K Godin (2017) Biofouling monitoring for aquatic invasive species (AIS) in DFO Maritimes Regions (Atlantic shore of Nova Scotia and southwest New Brunswick): May-November, 2012-2015. Canadian Technical Report of Fisheries and Aquatic Sciences 3158: 72 pp. Stephenson EH, RS Steneck, RH Seeley (2009) Possible temperature limits to range expansion of non-native Asian shore crabs in Maine. Journal of Experimental Marine Biology and Ecology 375: 21–31. doi:10.1016/j.jembe.2009.04.020

Aquatic invasive species (AIS) are non-native species that pose ecological and/or economic threats to Canada's coastal waters and resources. In response, Fisheries and Oceans Canada (DFO) established a program to detect and track the spread of aquatic invasive species in Canadian waters (2006). In the Newfoundland and Labrador (NL) Region, these species include biofouling organisms (tunicates, bryozoans, crustaceans, seaweeds), European Green Crab, and recently, several freshwater species (data not available at this time). DFO NL Science monitors for AIS in partnership with other DFO branches, the provincial government, Memorial University, including the Marine Institute, industry, first nations, and NGOs. Methods for detecting AIS used by DFO and their partners, include settlement plates, trapping, seining, eDNA (water samples), qPCR (species confirmation), SCUBA surveys along wharves, floating docks and vessel hulls, and video surveys at high-risk harbours. The data collected from DFO's monitoring program and their partners provides an overview of the distribution of AIS in the NL Region. This information can be used by the general public, scientists, and DFO managers. AIS NL Biofouling Species Fisheries and Oceans Canada's (DFO) National Marine Biofouling Monitoring Program conducts annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and some non-native species in Newfoundland and Labrador (NL) Region since 2006. Standardized monitoring protocols employed by DFO's NL, Maritimes, Gulf, and Quebec regions include biofouling collector plates deployed from May to October at georeferenced intertidal and shallow subtidal sites, including public docks, and public and private marinas and nautical clubs. Initially, (2006-2017), the collectors consisted of three 10 cm by 10 cm PVC plates deployed in a vertical array and spaced approximately 40 cm apart, with the shallowest plate suspended at least 1 m below the surface to sample subtidal and shallow intertidal species (McKenzie et al 2016a). Three replicate arrays were deployed at least 5 m apart per site. Since 2018, collector networks have been modified to improve statistical replication, including up to 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. Since 2006, seven invasive biofouling organisms have been detected in Newfoundland and Labrador harbours, marinas and coastal areas. Should be cited as follows: DFO Newfoundland and Labrador Region Aquatic Invasive Species Marine Biofouling Monitoring Program. Published March 2024. Coastal and Freshwater Ecology, Northwest Atlantic Fisheries Centre, Fisheries and Oceans Canada, St. John’s, Newfoundland and Labrador. Reference: Tunicates Golden star tunicate (Botryllus schlosseri) 2006 The Golden star tunicate was the first invasive tunicate detected in NL waters. It was reported in Argentia by the US Navy around 1945. It was found in 2006 on wharf structures in Argentia, Placentia Bay during the first AIS survey (Callahan et al 2010). This colonial tunicate is recognized by it star shaped grouping of individuals within the colony. It is currently found in Placentia Bay, Fortune Bay, St. Mary’s Bay, Conception Bay and the west coast of NL. The data provided here indicates the detections of this AIS in coastal NL. Violet tunicate (Botrylloides violaceus) 2007 The violet tunicate was first detected in NL waters in 2007 in Belleoram, Fortune Bay on wharf structures and vessels (McKenzie et al. 2016b). This colonial tunicate forms irregular shaped colonies usually of a solid color (orange, purple, yellow or cream). It is currently found in relatively small colonies in four harbours in NL; Placentia Bay (1), Fortune Bay (1), Conception Bay (1) and the west coast of NL (2). The data provided here indicates the detections of this AIS in coastal NL. Vase tunicate (Ciona intestinalis) 2012 The Vase tunicate, is

Fisheries and Oceans Canada (DFO) has been conducting surface water trawl surveys since 1992 in the coastal waters of British Columbia, Washington, Oregon and Alaska and in the high seas of the Gulf of Alaska. These surveys initially focused on determining the migratory patterns (1992-2002) and on the growth and physiology (2003-2016) of juvenile Pacific Salmon. Since 2016, these surveys have been broadened to monitor the whole pelagic ecosystem, retaining a focus on juvenile Pacific Salmon. Surveys have been conducted on the continental shelf of north and west Vancouver Island, included associated sounds and inlets since 1992 and are ongoing. These data are for tows conducted in the continental shelf area for depths shallower than 400 meters.

PURPOSE: The research survey provides a fisheries-independent source of information about all marine living organisms that are captured by the fishing trawl used to obtain samples in the southern Gulf of St. Lawrence. DESCRIPTION: Tow, catch, length frequency, and biological information for fish caught during the annual September research vessel trawl surveys in the southern Gulf of St. Lawrence (NAFO Division 4T). Abundance indices and spatial distribution patterns of commercial and non-commercial groundfish. The catch data that appear in this dataset SHOULD NOT BE USED FOR ECOLOGICAL ANALYSES INVOLVING CATCH RATES. Important factors such as vessel, fishing gear and diurnal periods must be accounted for to use these data in analyses. Please contact the data custodians if you are interested in using this data for any kind of ecological analyses involving catch rates. PARAMETERS COLLECTED: abundance estimates (ecological); distribution (ecological); species counts (ecological); gear (fishing); vessel information (fishing); point (spatial) NOTES ON QUALITY CONTROL: Scientific names listed in the survey species list have been mapped to recognized standards - marine taxa have been mapped to the World Register of Marine Species (WoRMS) using their online taxon match tool. All sampling locations were plotted on a map to perform a visual check confirming that the latitude and longitude coordinates were within the described sampling area. In 2003, because of a fire aboard the Alfred Needler, the Wilfred Templeman was used for the survey. However, no comparative fishing experiments have been conducted between the Alfred Needler and the Wilfred Templeman. We are therefore unable to integrate the indices derived for 2003 to the remainder of the survey time-series. SAMPLING METHODS: Sampling Method: Consult the "Protocols for research vessel cruises within the Gulf Region (dermersal fish) (1970-1980)" report, link provided in the citations list. USE LIMITATION: To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

Tow, catch, and length frequency for fish caught during the August sentinel surveys in the southern Gulf of St. Lawrence (NAFO Division 4T). Abundance indices and spatial distribution patterns of commercial groundfish. Note: Due to delays caused by logistic complexities and Covid the project did not take place in 2020

Fisheries and Oceans Canada (DFO) conducts an annual multidisciplinary scientific survey with a bottom trawl in the Estuary and the northern Gulf of St. Lawrence since 1978. Over the years, this survey has been conducted on four vessels: the MV Gadus Atlantica (1978-1994), the MV Lady Hammond (1984-1990), the CCGS Alfred Needler (1990-2005) and the CCGS Teleost (2004-2021). It is important to note that the objectives, the methods used and the identification of the species during these surveys have improved over time in response to DFO requests and mandates. The data are therefore not directly comparable between these surveys. The specificities of the missions onboard the MV Lady Hammond are described below. Objectives: 1. Assess groundfish populations abundance and condition 2. Assess environmental conditions 3. Inventory species biodiversity 4. Monitor the pelagic ecosystem 5. Collect samples for various research projects Survey description The survey covers the northern Gulf of St. Lawrence, that is the divisions 4R, 4S and the northern part of division 4T of the Northwest Atlantic Fisheries Organization (NAFO). A stratified random sampling strategy is used for this survey and the fishing gear used on the MV Lady Hammond is a bottom trawl Western IIA. Standard trawling tows last 30 minutes, starting from the time the trawl touches the sea floor. Towing speed is 3.5 knots. Data For each fishing tow, the catch is sorted and weighed by taxa; individuals are counted and biological data are then collected. For fish, crab and squid, size and weight are gathered by individual and, for some species, sex, gonad maturity, and the weight of certain organs (stomach, liver, gonads) are also evaluated. The soft rays of the anal fin are counted for redfish and otoliths are collected for redfish and Atlantic cod. Invertebrates are weighted and counted (no individual measurements). The catches per tow for fish taxa are available below. For more information please contact the data management team (gddaiss-dmsaisb@dfo-mpo.gc.ca).

Fisheries and Oceans Canada (DFO) conducts an annual multidisciplinary scientific survey with a bottom trawl in the Estuary and the northern Gulf of St. Lawrence since 1978. Over the years this survey has been conducted on cinq vessels: the MV Gadus Atlantica (1978-1994), the MV Lady Hammond (1984-1990), the CCGS Alfred Needler (1990-2005), the CCGS Teleost (2004-2022) and the CCGS Cabot (2022-present). It is important to note that the objectives, the methods used and the identification of the species during these surveys have improved over time in response to DFO requests and mandates. The data are therefore not directly comparable between these surveys. The specificities of the missions onboard the CCGS Teleost are described below. Objectives: 1. Assess groundfish and northern shrimp population abundance and condition 2. Assess environmental conditions 3. Inventory species biodiversity 4. Assess phytoplankton and mesozooplankton abundance 5. Monitor the pelagic ecosystem 6. Inventory marine mammals populations 7. Inventory seabirds populations 8. Collect samples for various research projects Survey description The survey covers the Estuary and the northern Gulf of St. Lawrence, that is the divisions 4R, 4S and the northern part of division 4T of the Northwest Atlantic Fisheries Organization (NAFO). Since 2008, coverage of division 4T has been increased in the upstream part of the Lower Estuary in order to sample the depths between 37 and 183 m. A stratified random sampling strategy is used for this survey and the area of the study area is 118,587 km². The fishing gear used on the CCGS Teleost is a four-sided Campelen 1800 shrimp trawl equipped with a Rockhopper footgear (“bicycle”). The trawl lengthening and codend are equipped with a 12.7-mm knotless nylon lining. Standard trawling tows last 15 minutes, starting from the time the trawl touches the sea floor. The aimed towing speed is 3 knots. Data For each fishing tow, the catch is sorted and weighed by taxa; individual are then count and biological data are then collected on a subsample. For fish, crab and squid, size and weight are gathered by individual and, for some species, sex, gonad maturity, and the weight of certain organs (stomach, liver, gonads) are also evaluated. The soft rays of the anal fin are counted for redfish, and the otoliths are sampled for several species such as Atlantic cod, Atlantic halibut, and witch flounder. A roughly 2-kg shrimp sample is sorted and weighed by species (and by stage of maturity for northern shrimp). The shrimps are measured individually. The other invertebrates are counted (no individual measurements) and photographed. The catches per tow for fish taxa are available below. This data is also available via the 'Biodiversity' application on the St. Lawrence Global Observatory (SLGO). For more information please contact the data management team (gddaiss-dmsaisb@dfo-mpo.gc.ca).

Fisheries and Oceans Canada (DFO) has been conducting surface water trawl surveys since 1992 in the coastal waters of British Columbia, Washington, Oregon and Alaska and in the high seas of the Gulf of Alaska. These surveys initially focused on determining the migratory patterns (1992-2002) and on the growth and physiology (2003-2016) of juvenile Pacific Salmon. Since 2016, these surveys have been broadened to monitor the whole pelagic ecosystem, retaining a focus on juvenile Pacific Salmon. In this record, surveys were conducted in continental slope waters at depths greater than 400 m out to the Gulf of Alaska, between 1995 and 2011. Periodically, there are tows from surveys conducted in other regions that occurred on the continental slope and they are included here. This set of data also includes data collected during the International Year of Salmon survey in 2022.