AIS NL Biofouling Species Fisheries and Oceans Canada's (DFO) National Marine Biofouling Monitoring Program conducts annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and some non-native species in Newfoundland and Labrador (NL) Region since 2006. Standardized monitoring protocols employed by DFO's NL, Maritimes, Gulf, and Quebec regions include biofouling collector plates deployed from May to October at georeferenced intertidal and shallow subtidal sites, including public docks, and public and private marinas and nautical clubs. Initially, (2006-2017), the collectors consisted of three 10 cm by 10 cm PVC plates deployed in a vertical array and spaced approximately 40 cm apart, with the shallowest plate suspended at least 1 m below the surface to sample subtidal and shallow intertidal species (McKenzie et al 2016a). Three replicate arrays were deployed at least 5 m apart per site. Since 2018, collector networks have been modified to improve statistical replication, including up to 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. Since 2006, seven invasive biofouling organisms have been detected in Newfoundland and Labrador harbours, marinas and coastal areas. Should be cited as follows: DFO Newfoundland and Labrador Region Aquatic Invasive Species Marine Biofouling Monitoring Program. Published March 2024. Coastal and Freshwater Ecology, Northwest Atlantic Fisheries Centre, Fisheries and Oceans Canada, St. John’s, Newfoundland and Labrador. Reference: Tunicates Golden star tunicate (Botryllus schlosseri) 2006 The Golden star tunicate was the first invasive tunicate detected in NL waters. It was reported in Argentia by the US Navy around 1945. It was found in 2006 on wharf structures in Argentia, Placentia Bay during the first AIS survey (Callahan et al 2010). This colonial tunicate is recognized by it star shaped grouping of individuals within the colony. It is currently found in Placentia Bay, Fortune Bay, St. Mary’s Bay, Conception Bay and the west coast of NL. The data provided here indicates the detections of this AIS in coastal NL. From 2018-2022, the Coastal Environmental Baseline Program provided additional support to enhance sampling efforts in Placentia Bay.

AIS NL Biofouling Species Fisheries and Oceans Canada's (DFO) National Marine Biofouling Monitoring Program conducts annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and some non-native species in Newfoundland and Labrador (NL) Region since 2006. Standardized monitoring protocols employed by DFO's NL, Maritimes, Gulf, and Quebec regions include biofouling collector plates deployed from May to October at georeferenced intertidal and shallow subtidal sites, including public docks, and public and private marinas and nautical clubs. Initially, (2006-2017), the collectors consisted of three 10 cm by 10 cm PVC plates deployed in a vertical array and spaced approximately 40 cm apart, with the shallowest plate suspended at least 1 m below the surface to sample subtidal and shallow intertidal species (McKenzie et al 2016a). Three replicate arrays were deployed at least 5 m apart per site. Since 2018, collector networks have been modified to improve statistical replication, including up to 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. Since 2006, seven invasive biofouling organisms have been detected in Newfoundland and Labrador harbours, marinas and coastal areas. Should be cited as follows: DFO Newfoundland and Labrador Region Aquatic Invasive Species Marine Biofouling Monitoring Program. Published March 2024. Coastal and Freshwater Ecology, Northwest Atlantic Fisheries Centre, Fisheries and Oceans Canada, St. John’s, Newfoundland and Labrador. Reference: Tunicates Vase tunicate (Ciona intestinalis) 2012 The Vase tunicate, is a high impact solitary invader and was first detected by DFO in 2012 on the Burin Peninsula at Ship Cove and Little Bay, Placentia Bay. Various mitigation measures (McKenzie et al. 2016b) contained this invasive tunicate to a small area for six years within Placentia Bay. First detected in Fortune Bay as an established population in 2019, increasing reports of the Vase tunicate have been made along the south coast of Newfoundland. The data provided here indicates the detections of this AIS in coastal NL. From 2018-2022, the Coastal Environmental Baseline Program provided additional support to enhance sampling efforts in Placentia Bay.

Fisheries and Oceans Canada’s (DFO) National Biofouling Monitoring Program (BMP) has conducted annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and non-indigenous species (NIS) since 2006. Standardized monitoring protocols employed by DFO-Maritimes, -Gulf, and -Quebec Regions include biofouling collector plates deployed from May to October at intertidal and shallow subtidal, geo-referenced sites, including public and private docks, aquaculture lease sites, public and private marinas and yacht clubs. Initially in the Maritimes Region (2006-2017) collectors consisted of 3, 10 cm by 10cm PVC plates deployed in a vertical array and spaced approximately 40-cm apart with the shallowest plate hung at least 1 m below the surface to sample shallow subtidal and intertidal species (Sephton et al. 2011, 2017). Two replicate arrays were deployed at least 5 m apart per site. Since 2018, collector arrays were modified to enhance statistical replication, including 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. The percent cover of AIS on all collectors was determined by visual examination and scored as follows; (i) ‘0’ = absent, (ii) ‘1’ = ≤25 % cover, (iii) ‘2’ = 25 to ≤50 %, (iv) ‘3’ = 50–75% , and (v) ‘4’ = >75%. Average percent cover is provided for all NIS observed annually per site. Presence-absence indicates that an NIS was observed on at least one collector plate. One additional rocky intertidal species (Asian shore crab; Hemigrapsus sanguineus) was assessed via beach surveys as permitted by time and resources following its initial siting in St Mary’s Bay (Nova Scotia) in April 2020. Rapid assessment surveys conducted in the Fall of 2020 and 2021 were employed to delineate H. sanguineus’ distribution and relative abundance. Areas deemed suitable and at high risk for spread were targeted, including exposed rocky intertidal habitat in southwest regions of Nova Scotia and New Brunswick. Each rapid assessment consisted of 30-minute beach surveys per site conducted by 2 or 3 people (modified from Stephenson et al. 2011). During each survey, crabs were collected under rocks and seaweed in preferred cobble/boulder habitat (Lohrer et al. 2000). Count data was standardized for each site as the number of crabs collected per 30-min search per person. Cite as: DFO-Maritimes Biofouling Monitoring Program. Published October 2018, Updated December 2023. Coastal Ecosystems Science Division, Fisheries and Oceans Canada, Dartmouth, NS Citations: Sephton D, B Vercaemer, JM Nicolas, J Keays (2011) Monitoring for invasive tunicates in Nova Scotia, Canada (2006-2009) Aquatic Invasions 6: 391-403. Sephton D, B Vercaemer, A Silva, L Stiles, M Harris, K Godin (2017) Biofouling monitoring for aquatic invasive species (AIS) in DFO Maritimes Regions (Atlantic shore of Nova Scotia and southwest New Brunswick): May-November, 2012-2015. Canadian Technical Report of Fisheries and Aquatic Sciences 3158: 72 pp. Stephenson EH, RS Steneck, RH Seeley (2009) Possible temperature limits to range expansion of non-native Asian shore crabs in Maine. Journal of Experimental Marine Biology and Ecology 375: 21–31. doi:10.1016/j.jembe.2009.04.020

Aquatic invasive species (AIS) are non-native species that pose ecological and/or economic threats to Canada's coastal waters and resources. In response, Fisheries and Oceans Canada (DFO) established a program to detect and track the spread of aquatic invasive species in Canadian waters (2006). In the Newfoundland and Labrador (NL) Region, these species include biofouling organisms (tunicates, bryozoans, crustaceans, seaweeds), European Green Crab, and recently, several freshwater species (data not available at this time). DFO NL Science monitors for AIS in partnership with other DFO branches, the provincial government, Memorial University, including the Marine Institute, industry, first nations, and NGOs. Methods for detecting AIS used by DFO and their partners, include settlement plates, trapping, seining, eDNA (water samples), qPCR (species confirmation), SCUBA surveys along wharves, floating docks and vessel hulls, and video surveys at high-risk harbours. The data collected from DFO's monitoring program and their partners provides an overview of the distribution of AIS in the NL Region. This information can be used by the general public, scientists, and DFO managers. AIS NL Biofouling Species Fisheries and Oceans Canada's (DFO) National Marine Biofouling Monitoring Program conducts annual field surveys to monitor the introduction, establishment, spread, species richness, and relative abundance of native and some non-native species in Newfoundland and Labrador (NL) Region since 2006. Standardized monitoring protocols employed by DFO's NL, Maritimes, Gulf, and Quebec regions include biofouling collector plates deployed from May to October at georeferenced intertidal and shallow subtidal sites, including public docks, and public and private marinas and nautical clubs. Initially, (2006-2017), the collectors consisted of three 10 cm by 10 cm PVC plates deployed in a vertical array and spaced approximately 40 cm apart, with the shallowest plate suspended at least 1 m below the surface to sample subtidal and shallow intertidal species (McKenzie et al 2016a). Three replicate arrays were deployed at least 5 m apart per site. Since 2018, collector networks have been modified to improve statistical replication, including up to 10 individual collectors deployed per site at 1 m depth and at least 5 m apart (as above) from May to October. Since 2006, seven invasive biofouling organisms have been detected in Newfoundland and Labrador harbours, marinas and coastal areas. Should be cited as follows: DFO Newfoundland and Labrador Region Aquatic Invasive Species Marine Biofouling Monitoring Program. Published March 2024. Coastal and Freshwater Ecology, Northwest Atlantic Fisheries Centre, Fisheries and Oceans Canada, St. John’s, Newfoundland and Labrador. Reference: Tunicates Golden star tunicate (Botryllus schlosseri) 2006 The Golden star tunicate was the first invasive tunicate detected in NL waters. It was reported in Argentia by the US Navy around 1945. It was found in 2006 on wharf structures in Argentia, Placentia Bay during the first AIS survey (Callahan et al 2010). This colonial tunicate is recognized by it star shaped grouping of individuals within the colony. It is currently found in Placentia Bay, Fortune Bay, St. Mary’s Bay, Conception Bay and the west coast of NL. The data provided here indicates the detections of this AIS in coastal NL. Violet tunicate (Botrylloides violaceus) 2007 The violet tunicate was first detected in NL waters in 2007 in Belleoram, Fortune Bay on wharf structures and vessels (McKenzie et al. 2016b). This colonial tunicate forms irregular shaped colonies usually of a solid color (orange, purple, yellow or cream). It is currently found in relatively small colonies in four harbours in NL; Placentia Bay (1), Fortune Bay (1), Conception Bay (1) and the west coast of NL (2). The data provided here indicates the detections of this AIS in coastal NL. Vase tunicate (Ciona intestinalis) 2012 The Vase tunicate, is

Fisheries and Oceans Canada (DFO) has been conducting surface water trawl surveys since 1992 in the coastal waters of British Columbia, Washington, Oregon and Alaska and in the high seas of the Gulf of Alaska. These surveys initially focused on determining the migratory patterns (1992-2002) and on the growth and physiology (2003-2016) of juvenile Pacific Salmon. Since 2016, these surveys have been broadened to monitor the whole pelagic ecosystem, retaining a focus on juvenile Pacific Salmon. Surveys have been conducted on the continental shelf of north and west Vancouver Island, included associated sounds and inlets since 1992 and are ongoing. These data are for tows conducted in the continental shelf area for depths shallower than 400 meters.

PURPOSE: The research survey provides a fisheries-independent source of information about all marine living organisms that are captured by the fishing trawl used to obtain samples in the southern Gulf of St. Lawrence. DESCRIPTION: Tow, catch, length frequency, and biological information for fish caught during the annual September research vessel trawl surveys in the southern Gulf of St. Lawrence (NAFO Division 4T). Abundance indices and spatial distribution patterns of commercial and non-commercial groundfish. The catch data that appear in this dataset SHOULD NOT BE USED FOR ECOLOGICAL ANALYSES INVOLVING CATCH RATES. Important factors such as vessel, fishing gear and diurnal periods must be accounted for to use these data in analyses. Please contact the data custodians if you are interested in using this data for any kind of ecological analyses involving catch rates. PARAMETERS COLLECTED: abundance estimates (ecological); distribution (ecological); species counts (ecological); gear (fishing); vessel information (fishing); point (spatial) NOTES ON QUALITY CONTROL: Scientific names listed in the survey species list have been mapped to recognized standards - marine taxa have been mapped to the World Register of Marine Species (WoRMS) using their online taxon match tool. All sampling locations were plotted on a map to perform a visual check confirming that the latitude and longitude coordinates were within the described sampling area. In 2003, because of a fire aboard the Alfred Needler, the Wilfred Templeman was used for the survey. However, no comparative fishing experiments have been conducted between the Alfred Needler and the Wilfred Templeman. We are therefore unable to integrate the indices derived for 2003 to the remainder of the survey time-series. SAMPLING METHODS: Sampling Method: Consult the "Protocols for research vessel cruises within the Gulf Region (dermersal fish) (1970-1980)" report, link provided in the citations list. USE LIMITATION: To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.

The objective of this project was to gather data to develop a model of the food web of the lower trophic levels of the nearshore area of the Beaufort Sea. Sampling took place from 2005 to 2008 using the CCGS Nahidik. The multidisciplinary character of the Nahidik program produced measurements of biology/ecology (primary production, phytoplankton, zooplankton, benthos, fish), chemical and physical oceanography, contaminants, geology and hydro acoustics. The data were collected in July and August of each year. The Nahidik program provided data to provide a baseline for future studies as well as an information source for environmental assessment.

Tow, catch, and length frequency for fish caught during the August sentinel surveys in the southern Gulf of St. Lawrence (NAFO Division 4T). Abundance indices and spatial distribution patterns of commercial groundfish. Note: Due to delays caused by logistic complexities and Covid the project did not take place in 2020

This project was completed by the Coastal Environmental Baseline Program (Coastal and Freshwater Ecology Section) in the Newfoundland and Labrador Science Branch of Fisheries and Oceans Canada (DFO). The nearshore fish and invertebrate community was sampled annually in Placentia Bay at 14 sites for 5 years (2018 – 2022). The objective of this program was to collect data on juvenile fish (specifically Atlantic cod) communities and habitats within Placentia Bay. The sites and timing (October) were selected based on historical sampling. There were several data types collected during each of these sampling trips. Boat-based beach seining was used to collect data on fish and invertebrate diversity, abundance, distribution, and size. Visual transects were used to identify vegetation and substrate (habitat) types and proportions at seine sites. CTD profiles (temperature, conductivity, depth, and salinity) were obtained using a castaway CTD. Finally, eDNA samples were collected at each site (2018-2021) to obtain complementary information on the presence of juvenile fish and other species present. This record contains the geographic locations of the 14 sites, and information on the timing and type of data collected at each site.

PURPOSE: Provide early detection of newly arrived Aquatic Invasive Species (AIS) and determine the spread, establishment and spatial distribution of existing AIS within marine waters of the southern Gulf of St. Lawrence (sGSL), DFO Gulf Region boundaries (northern and eastern coastal shores of NB, Gulf shore of NS, and PEI shoreline). DESCRIPTION: DFO Science monitors for AIS in the Gulf Region. The data collected from DFO's biofouling monitoring program provides an overview of the distribution and abundance of Aquatic Invasive Species (AIS) in the Gulf Region. This information can be used by the general public, scientists and DFO managers. Monitoring program targeting aquatic invasive species (AIS). Native biofouling species are not included in this dataset. Botrylloides violaceus: Violet tunicate Botryllus schlosseri: Golden star tunicate Ciona intestinalis: Vase tunicate Styela clava: Clubbed tunicate Caprella mutica*: Japanese skeleton shrimp Membranipora membranacea: Coffin box bryozoan Carcinus maenas*: European green crab Codium fragile*: Oyster thief alga *indicates species that are not included as percent cover as they are not accurately captured by the sampling method, but are included as detections. Included here is a dataset of detection and percent cover data of AIS, as well as a monitoring station dataset. Environmental data collected, including from temperature loggers, are stored but not included here. PARAMETERS COLLECTED: Air and water temperature, salinity, depth, dissolved oxygen, weather conditions, list of biofouling AIS, percent cover of AIS on PVC plates. NOTES ON QUALITY CONTROL: Each sample and species is processed and identified in a standardized fashion using standardized DFO Science AIS protocols and taxonomic references. Data is manually entered into DFO Gulf Region's AIS Science biofouling database and randomly verified for accuracy. SAMPLING METHODS: Biofouling monitoring is conducted using PVC collector plates that are deployed in the water column approximately 1 meter below the sea surface in the spring of each year. Biofouling organisms settle on these plates which are collected in the fall of the same year. Abundances of AIS are given as percent plate cover. Physico-chemical data including temperature, conductivity, and depth as well as weather conditions are noted at each geo-referenced biofouling monitoring site during initial deployment and at time of retrieval. All biofouling organisms settled on the underside of the PVC plates are noted and percent cover of each AIS is estimated. USE LIMITATION: To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.