This USGS data release includes data from experiments conducted with two species of caddisflies (Hydropsyche californica and Arctopsyche grandis (Order: Trichoptera) to quantify copper and cadmium uptake and loss after dissolved and dietary exposures. Both Arctopsyche and Hydropsyche belong to the family Hydropsychidae (O: Trichoptera). They are relatively sessile, net-spinning filter feeders. Data from these experiments can be used to characterize physiological parameters used in a bioaccumulation dynamic model.

These data were compiled for a mtDNA (CO1 gene) analysis of net-spinning caddisflies (Hydropsyche oslari) in the Colorado River Basin of the western United States. They were incorporated in a study that investigated the genetic diversity and population structure of H. oslari relative to the river network structure in the Upper and Lower Colorado River Basins. The data were collected from 2015 to 2016 through a collaboration with river runners. These citizen scientists collected insects by deploying light traps for one hour each night of their expedition. Light trap contents were preserved in ethanol on site and returned to the Southwest Biological Science Center in Flagstaff, Arizona for analysis in the laboratory.

Abstract The purpose of this sampling was to collect matched aquatic insect and water samples to support metals mixture toxicity modeling. This is part of a larger study that included exposing naturally colonized insect communities to metals mixtures in long-term laboratory toxicity tests, field surveys, and modeling (Schmidt and others, 2019). The Panther Creek sampling was conducted to give a field comparison to laboratory exposures of aquatic insect communities to cobalt (Co) plus copper (Cu) mixtures, the results of which were presented as part 4 of Schmidt and others (2019). The water samples from the Panther Creek area were collected to interpret metals concentrations in the tissues of mayflies and caddisflies relative to experimental exposures and to compare to community composition. Samples were collected from sites expected to give a gradient of metals concentrations, ranging from natural background reference sites to concentrations expected to be high enough to produce substantial bioaccumulation of metals. Results The results are presented in a workbook consisting of 6 datasheets which respectively include data on: 1. Summary: includes sampling locations and summarized matched water and tissue samples 2. Water-detailed: Field measurements and analytical results of water sampling 3. Tissue-detailed: Analytical results of insect tissue analyses 4. Benthic macroinvertebrates: Counts of benthic macroinvertebrate samples that coincided with the water and tissue samples 5. Water-QC: Results of quality control analyses associated with the water samples 6. Tissue-QC: Results of quality control analyses associated with the tissue samples For the 12 environmental stream samples from the Panther Creek watershed, Co values ranged from <0.01 to 329 µg/L and Cu values ranged from 0.2 to 24 µg/L. Dissolved organic carbon ranged from 0.72 to 2.2 mg/L, pH ranged from 7.1 to 8.8 units, alkalinity ranged from 8 to 50 mg/L as CaCO3, and hardness ranged from 17 to 167 mg/L as CaCO3. Approximate diel swings in pH for Panther Creek, measured at the Deep Creek campground prior to sunrise and in late afternoon were 0.4 units, and in Big Deer Creek at the mouth measured in early morning and late afternoon, the changes were less than 0.1 units. Cobalt and copper tissue concentrations ranged widely in the insects. Cobalt values ranged from 0.4 to 634 mg/kg dry weight in insect tissues and copper values ranged from 12 to 1080 mg/kg. Funding was from USGS Mineral Resources Program, and the Panther Creek sampling was conducted in cooperation with the Blackbird Mine Site Group. References Schmidt, T.S., Mebane, C.A., Miller, J.L., and Balistrieri, L.S., 2019, Effects of metal mixtures on aquatic insect communities in experimental streams: cadmium (Cd), cobalt (Co), copper (Cu), nickel (Ni), and zinc (Zn): US Geological Survey Data Release, also available at http://dx.doi.org/10.5066/P9XXBSAK

Abstract The purpose of this sampling was to collect matched aquatic insect and water samples to support metals mixture toxicity modeling. This is part of a larger study that included exposing naturally colonized insect communities to metals mixtures in long-term laboratory toxicity tests, field surveys, and modeling (Schmidt and others, 2019). The Panther Creek sampling was conducted to give a field comparison to laboratory exposures of aquatic insect communities to cobalt (Co) plus copper (Cu) mixtures, the results of which were presented as part 4 of Schmidt and others (2019). The water samples from the Panther Creek area were collected to interpret metals concentrations in the tissues of mayflies and caddisflies relative to experimental exposures and to compare to community composition. Samples were collected from sites expected to give a gradient of metals concentrations, ranging from natural background reference sites to concentrations expected to be high enough to produce substantial bioaccumulation of metals. Results The results are presented in a workbook consisting of 6 datasheets which respectively include data on: 1. Summary: includes sampling locations and summarized matched water and tissue samples 2. Water-detailed: Field measurements and analytical results of water sampling 3. Tissue-detailed: Analytical results of insect tissue analyses 4. Benthic macroinvertebrates: Counts of benthic macroinvertebrate samples that coincided with the water and tissue samples 5. Water-QC: Results of quality control analyses associated with the water samples 6. Tissue-QC: Results of quality control analyses associated with the tissue samples For the 12 environmental stream samples from the Panther Creek watershed, Co values ranged from <0.01 to 329 µg/L and Cu values ranged from 0.2 to 24 µg/L. Dissolved organic carbon ranged from 0.72 to 2.2 mg/L, pH ranged from 7.1 to 8.8 units, alkalinity ranged from 8 to 50 mg/L as CaCO3, and hardness ranged from 17 to 167 mg/L as CaCO3. Approximate diel swings in pH for Panther Creek, measured at the Deep Creek campground prior to sunrise and in late afternoon were 0.4 units, and in Big Deer Creek at the mouth measured in early morning and late afternoon, the changes were less than 0.1 units. Cobalt and copper tissue concentrations ranged widely in the insects. Cobalt values ranged from 0.4 to 634 mg/kg dry weight in insect tissues and copper values ranged from 12 to 1080 mg/kg. Funding was from USGS Mineral Resources Program, and the Panther Creek sampling was conducted in cooperation with the Blackbird Mine Site Group. References Schmidt, T.S., Mebane, C.A., Miller, J.L., and Balistrieri, L.S., 2019, Effects of metal mixtures on aquatic insect communities in experimental streams: cadmium (Cd), cobalt (Co), copper (Cu), nickel (Ni), and zinc (Zn): US Geological Survey Data Release, also available at http://dx.doi.org/10.5066/P9XXBSAK

These data are comprised of measurements of elements (e.g., uranium, cobalt, nickel, copper, zinc, cadmium, lead, etc.) in Canyon Mine containment pond invertebrate samples collected in calendar years 2017 and 2018.

These data are comprised of measurements of elements (e.g., uranium, cobalt, nickel, copper, zinc, cadmium, lead, etc.) in Canyon Mine containment pond invertebrate samples collected in calendar years 2017 and 2018.

These data describe the distribution and abundance of Hydropsyche, a widespread and diverse genus of net-spinning caddisflies, in the Colorado River Basin. Abundance data, measured as catch rates, were collected by citizen scientists using light traps. We subsampled Hydropsyche spp. from light trap samples collected throughout the Colorado River Basin and measured the lengths and widths of mesothoracic tibia and first tarsal segments. Samples and measurements were processed at Grand Canyon Monitoring and Research Center in Flagstaff, AZ.

These data are comprised of measurements of elements (e.g., uranium, cobalt, nickel, copper, zinc, cadmium, lead, etc.) in Pinyon Plain Mine containment pond invertebrate samples collected in calendar year 2022.

These data are comprised of measurements of elements (e.g., uranium, cobalt, nickel, copper, zinc, cadmium, lead, etc.) in Pinyon Plain Mine containment pond invertebrate samples collected in calendar year 2021.

These data are comprised of measurements of elements (e.g., uranium, cobalt, nickel, copper, zinc, cadmium, lead, etc.) in Pinyon Plain Mine containment pond invertebrate samples collected in calendar year 2022.