Abstract The purpose of this sampling was to collect matched aquatic insect and water samples to support metals mixture toxicity modeling. This is part of a larger study that included exposing naturally colonized insect communities to metals mixtures in long-term laboratory toxicity tests, field surveys, and modeling (Schmidt and others, 2019). The Panther Creek sampling was conducted to give a field comparison to laboratory exposures of aquatic insect communities to cobalt (Co) plus copper (Cu) mixtures, the results of which were presented as part 4 of Schmidt and others (2019). The water samples from the Panther Creek area were collected to interpret metals concentrations in the tissues of mayflies and caddisflies relative to experimental exposures and to compare to community composition. Samples were collected from sites expected to give a gradient of metals concentrations, ranging from natural background reference sites to concentrations expected to be high enough to produce substantial bioaccumulation of metals. Results The results are presented in a workbook consisting of 6 datasheets which respectively include data on: 1. Summary: includes sampling locations and summarized matched water and tissue samples 2. Water-detailed: Field measurements and analytical results of water sampling 3. Tissue-detailed: Analytical results of insect tissue analyses 4. Benthic macroinvertebrates: Counts of benthic macroinvertebrate samples that coincided with the water and tissue samples 5. Water-QC: Results of quality control analyses associated with the water samples 6. Tissue-QC: Results of quality control analyses associated with the tissue samples For the 12 environmental stream samples from the Panther Creek watershed, Co values ranged from <0.01 to 329 µg/L and Cu values ranged from 0.2 to 24 µg/L. Dissolved organic carbon ranged from 0.72 to 2.2 mg/L, pH ranged from 7.1 to 8.8 units, alkalinity ranged from 8 to 50 mg/L as CaCO3, and hardness ranged from 17 to 167 mg/L as CaCO3. Approximate diel swings in pH for Panther Creek, measured at the Deep Creek campground prior to sunrise and in late afternoon were 0.4 units, and in Big Deer Creek at the mouth measured in early morning and late afternoon, the changes were less than 0.1 units. Cobalt and copper tissue concentrations ranged widely in the insects. Cobalt values ranged from 0.4 to 634 mg/kg dry weight in insect tissues and copper values ranged from 12 to 1080 mg/kg. Funding was from USGS Mineral Resources Program, and the Panther Creek sampling was conducted in cooperation with the Blackbird Mine Site Group. References Schmidt, T.S., Mebane, C.A., Miller, J.L., and Balistrieri, L.S., 2019, Effects of metal mixtures on aquatic insect communities in experimental streams: cadmium (Cd), cobalt (Co), copper (Cu), nickel (Ni), and zinc (Zn): US Geological Survey Data Release, also available at http://dx.doi.org/10.5066/P9XXBSAK

This dataset is from the second of four experiments which test the toxicity of several metals with aquatic insect communities that were colonized in the field and then transferred and tested in experimental streams. Here we report original data from testing the toxicity of cadmium (Cd), zinc (Zn), and copper (Cu) singly and in mixtures, to natural aquatic insect communities. Thus, the exposures reproduced those in experiment 1, with the addition of a third metal, Cu Trays of cleaned gravel were placed in a stream (the Cache La Poudre River, Colorado) and allowed to colonize for about 40 days before being translocated to Aquatic Experimental Stream Laboratory (AXL) which was located at the USGS Fort Collins Science Center. There the insects were exposed for 30 days to metal mixtures in ratios and concentrations similar to those occurring in ambient conditions. Emerging adult insects were captured and collected daily throughout the experiment, while larvae and metal residues in periphyton, sediment, and Brachycentrus caddisflies (a common, large-bodied, robust taxa that could survive high metals accumulations) were collected on the final day of the experiment.

This dataset is from the second of four experiments which test the toxicity of several metals with aquatic insect communities that were colonized in the field and then transferred and tested in experimental streams. Here we report original data from testing the toxicity of cadmium (Cd), zinc (Zn), and copper (Cu) singly and in mixtures, to natural aquatic insect communities. Thus, the exposures reproduced those in experiment 1, with the addition of a third metal, Cu Trays of cleaned gravel were placed in a stream (the Cache La Poudre River, Colorado) and allowed to colonize for about 40 days before being translocated to Aquatic Experimental Stream Laboratory (AXL) which was located at the USGS Fort Collins Science Center. There the insects were exposed for 30 days to metal mixtures in ratios and concentrations similar to those occurring in ambient conditions. Emerging adult insects were captured and collected daily throughout the experiment, while larvae and metal residues in periphyton, sediment, and Brachycentrus caddisflies (a common, large-bodied, robust taxa that could survive high metals accumulations) were collected on the final day of the experiment.

Insect-Mediated Contaminant Flux model parameters and outputs. This dataset is associated with the following publication: Olson, C.I., G.B. Beaubien, R.R. Otter, D.M. Walters, and M.A. Mills. Ecotoxicological Studies Indicate That Sublethal and Lethal Processes Limit Insect-Mediated Contaminant Flux. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 42(9): 1982-1992, (2023).

Insect-Mediated Contaminant Flux model parameters and outputs. This dataset is associated with the following publication: Olson, C.I., G.B. Beaubien, R.R. Otter, D.M. Walters, and M.A. Mills. Ecotoxicological Studies Indicate That Sublethal and Lethal Processes Limit Insect-Mediated Contaminant Flux. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 42(9): 1982-1992, (2023).

This dataset is from the fourth of four experiments which test the toxicity of several metals with aquatic insect communities that were colonized in the field and then transferred and tested in experimental streams. Here we report original data from testing the toxicity of cobalt (Co), copper (Cu), and nickel (Ni), singly and in mixtures, to natural benthic communities including aquatic insect and algal communities. The methods are the same as those used in Experiment 3, except for the metals combinations. Trays of cleaned gravel were placed in a stream (the Cache La Poudre River, Colorado) and allowed to colonize for about 40 days before being translocated to Aquatic Experimental Stream Laboratory (AXL) which was located at the USGS Fort Collins Science Center. There the insects were exposed for 30 days to metal mixtures in ratios and concentrations similar to those occurring in ambient conditions. Emerging adult insects were captured and collected daily throughout the experiment, and larvae were collected at the end of the experiment. Additionally, metal residues were measured in sediments, periphyton and Brachycentrus caddisflies (a common, large-bodied, robust taxa that could survive high metals accumulations). Algal biomass responses to metals were measured in situ by in-vivo fluorescence.

This dataset is from the fourth of four experiments which test the toxicity of several metals with aquatic insect communities that were colonized in the field and then transferred and tested in experimental streams. Here we report original data from testing the toxicity of cobalt (Co), copper (Cu), and nickel (Ni), singly and in mixtures, to natural benthic communities including aquatic insect and algal communities. The methods are the same as those used in Experiment 3, except for the metals combinations. Trays of cleaned gravel were placed in a stream (the Cache La Poudre River, Colorado) and allowed to colonize for about 40 days before being translocated to Aquatic Experimental Stream Laboratory (AXL) which was located at the USGS Fort Collins Science Center. There the insects were exposed for 30 days to metal mixtures in ratios and concentrations similar to those occurring in ambient conditions. Emerging adult insects were captured and collected daily throughout the experiment, and larvae were collected at the end of the experiment. Additionally, metal residues were measured in sediments, periphyton and Brachycentrus caddisflies (a common, large-bodied, robust taxa that could survive high metals accumulations). Algal biomass responses to metals were measured in situ by in-vivo fluorescence.

These data are comprised of measurements of elements (e.g., uranium, cobalt, nickel, copper, zinc, cadmium, lead, etc.) in Canyon Mine containment pond invertebrate samples collected in calendar years 2017 and 2018.

These data are comprised of measurements of elements (e.g., uranium, cobalt, nickel, copper, zinc, cadmium, lead, etc.) in Canyon Mine containment pond invertebrate samples collected in calendar years 2017 and 2018.

This data release includes sampling location data, field-collected water chemistry data, cation and anion concentration data for water and tissues of submerged aquatic vegetation, aquatic insect larvae, adult aquatic insects and riparian spiders from 35 first- and second-order sub-alpine streams that ranged over several orders of magnitude in metal concentrations but were similar in elevation, geology, and stream morphology. Sampling was completed in late summer, after snowmelt runoff was complete and as the streams approached baseflow conditions.