Data pertaining to a NIS-expressing cell line, hNIS-HEK293T-EPA, and its screening capabilities for determining inhibitors of NIS-mediated iodide uptake. This dataset is associated with the following publication: Hallinger, D., A. Murr, A. Buckalew, S. Simmons, T. Stoker, and S. Laws. Development of a Screening Approach to Detect Thyroid Disrupting Chemicals that Inhibit the Human Sodium/Iodide Symporter (NIS). TOXICOLOGY IN VITRO. Elsevier Science Ltd, New York, NY, USA, 66-78, (2017).

No novel data were reported in association with this product. This dataset is not publicly accessible because: The associated publication is a review/forum-type article. No novel scientific data are reported. All data cited have been previously published elsewhere. It can be accessed through the following means: Not applicable. Format: This article is a review/forum-type article. No novel scientific data are included. This dataset is associated with the following publication: Knapen, D., E. Stinckens, J. Cavallin, G. Ankley, H. Holbech, D. Villeneuve, and L. Vergauwen. Toward an AOP network-based tiered testing strategy for the assessment of thyroid hormone disruption. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 54(16): 8491-8499, (2020).

Iodide uptake following a two-hour incubation with concentrations of 5-ATcompared to control iodide uptake in FRTL thyroid follicular cells. This dataset is associated with the following publication: Adams, V., M. Bazar, E. Reinke, A. Buckalew, and W. Eck. In vitro and in vivo effects of 5-Aminotetrazole (5-AT)- an energetic compound. REGULATORY TOXICOLOGY AND PHARMACOLOGY. Elsevier Science Ltd, New York, NY, USA, 111(104573): 1, (2020).

Dataset for the publication "Expanded High-Throughput Screening and Chemotype-Enrichment Analysis of the Phase II:e1k ToxCast Library for Human Sodium-Iodide Symporter (NIS) Inhibition". https://doi.org/10.1007/s00204-021-03006-2 Dataset1 contains data for figures. Dataset2 contains all supplemental tables data. This dataset is associated with the following publication: Wang, J., A. Richard, A. Murr, A. Buckalew, R. Lougee, M.A. Shobair, D. Hallinger, S. Laws, and T. Stoker. Expanded High-Throughput Screening and Chemotype-Enrichment Analysis of the Phase II:e1k ToxCast Library for Human Sodium-Iodide Symporter (NIS) Inhibition. Archives of Toxicology. Springer, New York, NY, USA, 95(5): 1723-1737, (2021).

How to access data for "Decrane R, Stoker T, Murr A, Ford J, El-Masri H. Cross species extrapolation of the disruption of thyroid hormone synthesis by oxyfluorfen using in vitro data, physiologically based pharmacokinetic (PBPK), and thyroid hormone kinetics models. Curr Res Toxicol. 2023 Nov 23;5:100138. doi: 10.1016/j.crtox.2023.100138. PMID: 38074188; PMCID: PMC10697989.". This dataset is not publicly accessible because: N/A. It can be accessed through the following means: Data will be made available on request from Hisham El-Masri (el-masri.hisham@epa.gov). Format: N/A. This dataset is associated with the following publication: Decrane, R., T. Stoker, A. Murr, J. Ford, and H. El-Masri. Cross species extrapolation of the disruption of thyroid hormone synthesis by oxyfluorfen using in vitro data, physiologically based pharmacokinetic (PBPK), and thyroid hormone kinetics models. Current Research in Toxicology. Elsevier B.V., Amsterdam, NETHERLANDS, 5: 100138, (2023).

This dataset was generated to support parameterization of a larval amphibian thyroid axis computational model. Specifically, plasma iodine levels were determined not only to inform a concentration value for model parameter specification in the model, but also to support the notion that aquatic and terrestrial species should be considered substantially different with regard to circulating iodide levels. Other computational model physical parameters were informed by evaluating Xenopus laevis thyroid gland histological images for morphological features including thyroid follicular cell numbers and areas. This dataset is associated with the following publication: Haselman, J., J. Nichols, K. Mattingly, M. Hornung, and S. Degitz. A biologically based computational model for the hypothalamic-pituitary-thyroid (HPT) axis in Xenopus laevis larvae. MATHEMATICAL BIOSCIENCES. Elsevier Science Ltd, New York, NY, USA, 362: 109021, (2023).

Data pertaining to a NIS-expressing cell line, FRTL-5 and it's screening capabilities for confirming inhibitors of NIS-mediated iodide uptake. This dataset is associated with the following publication: Buckalew, A., J. Wang, A. Murr, C. Deisenroth, W. Stewart, T. Stoker, and S. Laws. Evaluation of potential sodium-iodide symporter (NIS) inhibitors using a secondary Fischer rat thyroid follicular cell (FRTL-5) radioactive iodide uptake (RAIU) assay. Archives of Toxicology. Springer, New York, NY, USA, 94(3): 873-885, (2020).

180626 Thyroid Hormone PFOA PFOS Manuscript Data. This dataset is associated with the following publication: Selano, J., V. Richardson, J. Washington, and C. Mazur. Characterization of non-radiolabeled Thyroxine (T4) uptake in cryopreserved rat hepatocyte suspensions: Pharmacokinetic implications for PFOA and PFOS chemical exposure. TOXICOLOGY IN VITRO. Elsevier Science Ltd, New York, NY, USA, 58: 230-238, (2019).

Biomarker Image Cytometry. The cell-level frequency of NK2 Homeobox 1 (NKX2-1), Keratin 7 (KRT7), and Thyroglobulin (TG) protein staining were quantitatively evaluated by high-content imaging across huThyrEC cell line variants (1-4) in two medium formulations (huThyrEC and h7H) for verification of thyroid follicular epithelial cell enrichment. Data are the % positive expression frequency (mean ± SD) of two replicates. This dataset is associated with the following publication: Hopperstad, K., T. Truschel, T. Wahlicht, W. Stewart, A. Eicher, T. May, and C. Deisenroth. Characterization of Novel Human Immortalized Thyroid Follicular Epithelial Cell Lines. Applied In Vitro Toxicology. Mary Ann Liebert, Inc., Larchmont, NY, USA, 7(2): 39-49, (2021).