This dataset consists of trace organic contaminant concentrations and bacteria counts in wastewater effluents, and observed responses in fish exposed to effluents. The study was conducted from April to September 2022 at a facility located in Hutchinson, Minnesota. Nine pairs of primary-treated, activated sludge secondary treated, and membrane bioreactor secondary treated wastewater were characterized for select trace organic contaminants (pharmaceuticals, pesticides and pesticide degradates, hormones, alkylphenols, and bisphenols) and bacterial (total coliform, Escherichia coli) counts. Two 21-day exposures were conducted on-site in which fathead minnows were exposed to activated sludge secondary treated effluent and membrane bioreactor secondary treated effluent. Effluent samples were collected once per month in April, August, and September, and once weekly during the on-site exposures.

This dataset consists of trace organic contaminant concentrations and bacteria counts in wastewater effluents, and observed responses in fish exposed to effluents. The study was conducted from April to September 2022 at a facility located in Hutchinson, Minnesota. Nine pairs of primary-treated, activated sludge secondary treated, and membrane bioreactor secondary treated wastewater were characterized for select trace organic contaminants (pharmaceuticals, pesticides and pesticide degradates, hormones, alkylphenols, and bisphenols) and bacterial (total coliform, Escherichia coli) counts. Two 21-day exposures were conducted on-site in which fathead minnows were exposed to activated sludge secondary treated effluent and membrane bioreactor secondary treated effluent. Effluent samples were collected once per month in April, August, and September, and once weekly during the on-site exposures.

Water-borne contaminants were monitored in 69 tributaries of the Laurentian Great Lakes in 2010 and 2014 using semipermeable membrane devices (SPMDs), and polar organic chemical integrative samplers (POCIS). Analyses included 185 chemicals (143 detected) including PAHs, legacy and current-use pesticides, fire retardants, pharmaceuticals, fragrances, and others. Hazard quotients were calculated by dividing detected concentrations by biological effect concentrations reported in the ECOTOX Knowledgebase (Toxicity quotients, TQs) or ToxCast database (Exposure Activity Ratios, EARs). This dataset is associated with the following publication: Alvarez, D., S. Corsi, L. De Cicco, D. Villeneuve, and A. Baldwin. Identifying chemicals and mixtures of potential biological concern detected in passive samplers from Great Lakes tributaries using high-throughput data and biological pathways. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA,

The datafile contains data associated with analytical chemistry, fish gene expression, in vitro bioassays, chemical prioritization, and Attagene data for surface water monitoring and caged fish studies on the South Platte River (Colorado, USA) in 2018 and 2019. This dataset is associated with the following publication: Cavallin, J., J. Beihoffer, B. Blackwell, A. Cole, D. Ekman, R. Hofer, A. Jastrow, J. Kinsey, K. Keteles, E. Maloney, J. Parman, D. Winkelman, and D. Villeneuve. Effects-based monitoring of bioactive compounds associated with municipal wastewater treatment plant effluent discharge to the South Platte River, Colorado, USA. ENVIRONMENTAL POLLUTION. Elsevier Science Ltd, New York, NY, USA, 289: 117928, (2021).

The datafile contains data associated with analytical chemistry, fish gene expression, in vitro bioassays, chemical prioritization, and Attagene data for surface water monitoring and caged fish studies on the South Platte River (Colorado, USA) in 2018 and 2019. This dataset is associated with the following publication: Cavallin, J., J. Beihoffer, B. Blackwell, A. Cole, D. Ekman, R. Hofer, A. Jastrow, J. Kinsey, K. Keteles, E. Maloney, J. Parman, D. Winkelman, and D. Villeneuve. Effects-based monitoring of bioactive compounds associated with municipal wastewater treatment plant effluent discharge to the South Platte River, Colorado, USA. ENVIRONMENTAL POLLUTION. Elsevier Science Ltd, New York, NY, USA, 289: 117928, (2021).

Concentrations of a wide variety of environmental organic contaminants were measured in surface waters of the Lake Mead National Recreation Area (Nevada and Arizona) in 2010 to provide a measure of exposure to resident fish species. These contaminants were sampled using the semipermeable membrane device (SPMD) and the polar organic chemical integrative sampler (POCIS) which allow for the determination of the average contaminant concentrations over a month-long study period. Contaminants measured included polycyclic aromatic hydrocarbons, polychlorinated biphenyls, organochlorine pesticides, pharmaceuticals, select illicit drugs, and other chemicals related to wastewater treatment plant effluents. In addition, sediments, periphyton, fish feeds, and other materials from the Willow Beach fish hatchery were screened for polychlorinated biphenyls which are historically present in many hatcheries across the Nation.

Concentrations of a wide variety of environmental organic contaminants were measured in surface waters of the Lake Mead National Recreation Area (Nevada and Arizona) in 2010 to provide a measure of exposure to resident fish species. These contaminants were sampled using the semipermeable membrane device (SPMD) and the polar organic chemical integrative sampler (POCIS) which allow for the determination of the average contaminant concentrations over a month-long study period. Contaminants measured included polycyclic aromatic hydrocarbons, polychlorinated biphenyls, organochlorine pesticides, pharmaceuticals, select illicit drugs, and other chemicals related to wastewater treatment plant effluents. In addition, sediments, periphyton, fish feeds, and other materials from the Willow Beach fish hatchery were screened for polychlorinated biphenyls which are historically present in many hatcheries across the Nation.

The present study highlights the utility of bioeffects-based monitoring in conjunction with analytical chemical measurements of surface waters on the Colorado River associated with a historically bioactive wastewater treatment plant effluent. Concurrent with chemical monitoring and in vitro bioactivity measurements, in situ caged fish systems were employed to evaluate the potential bioavailability of predicted biologically-active contaminants associated with ER, GR, and PPAR-associated activities. The present study compares the effects of a wastewater treatment plant facility upgrade on bioactive contaminant loading. This dataset is associated with the following publication: Cavallin, J., W. Battaglin, J. Beihoffer, B. Blackwell, P. Bradley, A. Cole, D. Ekman, R. Hofer, J. Kinsey, K. Keteles, D. Winkelman, and D. Villeneuve. Effects-Based Monitoring of Bioactive Chemicals Discharged to the Colorado River Before and After a Municipal Wastewater Treatment Plant Replacement. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 55(2): 974-984, (2021).

Water and sediment was collected to assess the occurrence of contaminants of emerging concern (CECs) in National Park waters of the northern Colorado Plateau, USA. CEC presence in water and sediment is reported for 21 sites in eight U.S. national parks in the northern Colorado Plateau region. From 2012 to 2016, at least one PPCP and/or WWI was detected at most sites on over half of sampling visits, indicating that CECs are not uncommon even in isolated areas. Maximum concentrations in this study were generally below available water quality benchmarks, sediment quality guidelines, and concentrations known to induce biological activity in vitro. C occurrence patterns and similarities between continuous and isolated flow locations suggest that direct contamination from individual visitors may also occur. While the data indicate there is little aquatic health risk associated with CECs at our sites, results demonstrate the ubiquity of CECs on the landscape and a continued need for public outreach concerning resource-use ethics and the potential effects of upstream development. This dataset is associated with the following publication: Weissinger, R., B. Blackwell, K. Keteles, W. Battaglin, and P. Bradley. Bioactive contaminants of emerging concern in national park waters of the northern Colorado plateau, USA. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 636: 910-918, (2018).