The pictures in the power point file were taken using a confocal microscope with green and red filters which represent viable and dead bacteria cells, respectively. Additionally, we provided the detailed procedures of bacteria propagation and viability staining in a MS word file. The data may provide background/supporting information for other researchers who are planning to perform for microscopic bacteria viability assays. This dataset is associated with the following publication: Alvarez, N., R. Noga, S. Chae, G. Sorial, H. Ryu, and V. Shanov. Heatable carbon nanotube composite membranes for sustainable recovery from biofouling. Biofouling. Taylor & Francis Group, London, UK, 33(10): 847-854, (2017).

The bacteria sequence data generated in this study is available in the Short Read Archive (SRA) of NCBI (https://www.ncbi.nlm.nih.gov/) under BioProject PRJNA509718. This dataset is associated with the following publication: Siponen, S., J. Ikonen, V. Gomez-Alvarez, A. Hokajärvi, M. Ruokolainen, B. Jayaprakash, M. Kolehmainen, I.T. Miettinen, T. Pitkänen, and E. Torvinen. Effect of Pipe Material and Disinfectant on Active Bacterial Communities in Drinking Water and Biofilms. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, USA, 136(1): lxaf004, (2025).

Excel dataset including reduced data for Evaluation of Water Treatments on Surfaces to Inhibit Bacterial Spore Resuspension manuscript. This dataset is associated with the following publication: Hook, D.A., J. Gilberry, C. Johnson, R. Yaga, and J. Archer. Evaluation of Water Treatments on Surfaces to Inhibit Bacterial Spore Resuspension. Remediation Journal. John Wiley & Sons, Inc., Hoboken, NJ, USA, 34(3): e21779, (2024).

This study aimed to (1) enrich microbial acetylenotrophs from trichloroethylene (TCE) contaminated groundwater and (2) evaluate whether these enrichments could degrade TCE coupled to acetylene degradation. Acetylenotrophs are microorganisms that use acetylene as their carbon and energy source. TCE contaminated groundwater was collected from wells at the Naval Air Warfare Center (NAWC) in West Trenton, New Jersey. Microbial acetylene uptake in groundwater samples was established by mixing the groundwater with a defined mineral medium to supply nutrients and providing acetylene as the sole electron donor and carbon source. The structure of the microbial community in those enrichments was characterized as shown by 16S rRNA gene sequencing and analysis. The acetylenotrophic groundwater enrichment cultures were then tested to assess whether they could utilize acetylene to drive reduction of TCE and tetrachloroethene (PCE) to vinyl chloride.

This study aimed to (1) enrich microbial acetylenotrophs from trichloroethylene (TCE) contaminated groundwater and (2) evaluate whether these enrichments could degrade TCE coupled to acetylene degradation. Acetylenotrophs are microorganisms that use acetylene as their carbon and energy source. TCE contaminated groundwater was collected from wells at the Naval Air Warfare Center (NAWC) in West Trenton, New Jersey. Microbial acetylene uptake in groundwater samples was established by mixing the groundwater with a defined mineral medium to supply nutrients and providing acetylene as the sole electron donor and carbon source. The structure of the microbial community in those enrichments was characterized as shown by 16S rRNA gene sequencing and analysis. The acetylenotrophic groundwater enrichment cultures were then tested to assess whether they could utilize acetylene to drive reduction of TCE and tetrachloroethene (PCE) to vinyl chloride.

Carbon nanotubes (CNTs) have great potential in industrial, consumer, and mechanical applications, based partly on their unique structural, optical and electronic properties. CNTs are commonly oxidized or treated with surfactants to facilitate aqueous solution processing, and these CNT surface modifications also increase possible human and ecological exposures to nanoparticle-contaminated waters. To determine the exposure outcomes of oxidized and surfactant-wrapped multiwalled carbon nanotubes (MWCNTs) on biochemical processes, metabolomics based profiling of human liver cells (C3A) was utilized. Cells were exposed to 0, 10, or 100 ng/mL of MWCNTs for 24 and 48 hr. MWCNT particle size distribution, charge, and aggregation were monitored concurrently during exposures. Following MWCNT exposure, cellular metabolites were extracted, lyophilized, and buffered for 1H NMR analysis. Acquired spectra were subjected to both multivariate and univariate analysis to determine the consequences of nanotube exposure on the metabolite profile of C3A cells. Resulting scores plots illustrated temporal and dose-dependent metabolite responses to all MWCNTs tested. Loadings plots coupled with t-test filtered spectra identified metabolites of interest. XPS analysis revealed the presence of hydroxyl and carboxyl functionalities on both MWCNTs surfaces. Metal content analysis by ICP-AES indicated that the total mass concentration of the potentially toxic impurities in the exposure experiments were extremely low (i.e. [Ni] ≤ 2 × 10−10 g/mL). Preliminary data suggested that MWCNT exposure causes perturbations in biochemical processes involved in cellular oxidation as well as fluxes in amino acid metabolism and fatty acid synthesis. Dose-response trajectories were apparent and spectral peaks related to both dose and MWCNT dispersion methodologies were determined. Correlations of the significant changes in metabolites will help to identify potential biomarkers associated with carbonaceous nanoparticle exposure. This dataset is associated with the following publication: Henderson, M., D. Bouchard, X. Chang, S. Al-Abed, and Q. Teng. Biomarker analysis of liver cells exposed to surfactant-wrapped and oxidized multi-walled carbon nanotubes (MWCNTs). SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 565: 777–786, (2016).

Recovery Data for non-spore-forming bacteria, used as surrogates for select agents in surface sampling laboratory tests. Three surface sampling devices (3M sponge stick, LabPlas Sani-stick, and Solar Cult sponge, were used to attempt collection of E. coli, Y. ruckerii, and S. marcescens on Glass, ABS Plastic, and Stainless Steel. These data are useful for selection of surface sampling methods following a contamination incident. This dataset is associated with the following publication: Aslett, L., M. Calfee, M. Monge, A. Abdel-Hady, T. Chamberlain, R. Baartmans, and A. Touati. Evaluation of Sponge Wipe Surface Sampling for Collection of Potential Surrogates for Non-spore-forming Bioterrorism Agents. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, USA, 135(5): lxae097, (2024).

Decontamination of Bacillus spores adhered to common drinking water infrastructure surfaces was evaluated using a variety of disinfectants. Corroded iron and cement-mortar lined iron represented the infrastructure surfaces, and were conditioned in a 23 m long, 15 cm diameter (75 ft long, 6 in diameter) pilot-scale drinking water distribution pipe system. Decontamination was evaluated using increased water velocity (flushing) alone at 0.5 m sec-1 (1.7 ft sec-1), as well as free chlorine (5 and 25 mg L-1), monochloramine (25 mg L-1), chlorine dioxide (5 and 25 mg L-1), ozone (2.0 mg L-1), peracetic acid 25 mg L-1) and acidified nitrite (0.1 mol L-1 at pH 2 and 3), all followed by flushing at 0.3 m sec-1 (1 ft sec-1). Flushing alone reduced the adhered spores by 0.5 and 2.0 log10 from iron and cement-mortar, respectively. Log10 reduction on corroded iron pipe wall coupons ranged from 1.0 to 2.9 at respective chlorine dioxide concentrations of 5 and 25 mg L-1, although spores were undetectable on the iron surface during disinfection at 25 mg L-1. Acidified nitrite (pH 2, 0.1 mol L-1) yielded no detectable spores on the iron surface during the flushing phase after disinfection. Chlorine dioxide was the best performing disinfectant with >3.0 log10 removal from cement-mortar at 5 and 25 mg L-1.The data show that free chlorine, monochloramine, ozone and chlorine dioxide followed by flushing can reduce adhered spores by >3.0 log10 on cement-mortar. This dataset is associated with the following publication: Szabo , J., G. Meiners, L. Heckman, G. Rice , and J. Hall. Decontamination of Bacillus spores adhered to iron and cement-mortar drinking water infrastructure in a model system using disinfectants. JOURNAL OF ENVIRONMENTAL MANAGEMENT. Elsevier Science Ltd, New York, NY, USA, 187: 1-7, (2017).

This study involves the use of biofiltration, which is a control technique to degrade trihalomethanes using a bioreactor containing living material to capture and biologically degrade pollutants. Common uses include processing waste water, capturing harmful chemicals or silt from surface runoff, and microbiotic oxidation of contaminants in air. This dataset is associated with the following publication: Mezgebe, B., G. Sorial, D. Wendell, and E. Sahle-Demessie. Effectiveness of biosurfactant for the removal of trihalomethanes by biotrickling filter. Wiley StatsRef: Statistics Reference Online. John Wiley & Sons, Inc., Hoboken, NJ, USA, 1(1): 12031, (2019).