This data release presents chemical and biological results from investigations of water quality, fish endocrine disruption, and emergent insects in the Shenandoah River Watershed (Virginia and West Virginia, USA) conducted during 2014, 2015, and 2016. Multiple sampling campaigns were conducted at sites located throughout the Shenandoah River Watershed (Table 1). The complex inorganic and organic chemical characteristics of river waters and wastewater treatment plant effluents were characterized using 21 separate analytical methods at 7 laboratories (Tables 2, 3, and 4). To assess the relations between water composition and fish endocrine disruption, 21-day mobile laboratory adult fathead minnow exposure experiments with water quality and biological characterization (water collected on day 0, 7, 14, and 21; fish collected on day 0, 7, and 21) were conducted at 4 locations from August 12 to September 1, 2014, 3 locations from May 26 to June 16, 2015, and 5 locations from July 27 to August 17, 2016 (Tables 1 and 5). To assess changes in water chemistry resulting from point and non-point sources along the flow path, targeted stream-reach sampling for water quality characterization was conducted at 14 locations from August 25 to August 27, 2015 (Tables 1 and 6). Temporal variability was assessed by time-series sampling for water quality characterization at 2 locations associated with wild fish collection from April 4 to June 14, 2016 (Tables 1 and 7). Spatial variability across the watershed was assessed by landscape-directed sampling for water-quality characterization with biological assessment at 18 locations from August 9 to August 16, 2016, and 17 locations from October 17 to October 18, 2016 (Tables 1 and 8). Biological results for the 21-day mobile-laboratory adult fathead minnow exposure experiments are presented in Table 9. Biological results for the wild smallmouth bass and white sucker sampling on April 12, 2016 are presented in Table 10. Biological results for the 96-hour larval fathead minnow exposures from July, August, and October 2016 are presented in Table 11. Results for tissue analysis of pesticides and disinfection byproducts in adult fathead minnows collected at day 21 of the August to September 2014 exposure experiments are presented in Table 12. Emergent aquatic insects, terrestrial insects, and spider sampling was conducted at 4 locations from May 21 to May 25, 2015 (Tables 13, 14, and 15) and tissue samples analyzed for pesticides (Tables 16 and 17).

This data release presents the results of analyses of biota and water samples collected on multiple dates from 2007 to 2014 at 3 locations in Lake Mead National Recreation Area. Data are presented in 3 spreadsheets containing sample analyses for (1) stable isotopes in biota (2007-2014), (2) synthetic organic compounds in biota (2013-2014), and (3) synthetic organic compounds in water (2013-2014)

This data release presents the results of analyses of biota and water samples collected on multiple dates from 2007 to 2014 at 3 locations in Lake Mead National Recreation Area. Data are presented in 3 spreadsheets containing sample analyses for (1) stable isotopes in biota (2007-2014), (2) synthetic organic compounds in biota (2013-2014), and (3) synthetic organic compounds in water (2013-2014)

The datafile contains data associated with analytical chemistry, fish gene expression, in vitro bioassays, chemical prioritization, and Attagene data for surface water monitoring and caged fish studies on the South Platte River (Colorado, USA) in 2018 and 2019. This dataset is associated with the following publication: Cavallin, J., J. Beihoffer, B. Blackwell, A. Cole, D. Ekman, R. Hofer, A. Jastrow, J. Kinsey, K. Keteles, E. Maloney, J. Parman, D. Winkelman, and D. Villeneuve. Effects-based monitoring of bioactive compounds associated with municipal wastewater treatment plant effluent discharge to the South Platte River, Colorado, USA. ENVIRONMENTAL POLLUTION. Elsevier Science Ltd, New York, NY, USA, 289: 117928, (2021).

The datafile contains data associated with analytical chemistry, fish gene expression, in vitro bioassays, chemical prioritization, and Attagene data for surface water monitoring and caged fish studies on the South Platte River (Colorado, USA) in 2018 and 2019. This dataset is associated with the following publication: Cavallin, J., J. Beihoffer, B. Blackwell, A. Cole, D. Ekman, R. Hofer, A. Jastrow, J. Kinsey, K. Keteles, E. Maloney, J. Parman, D. Winkelman, and D. Villeneuve. Effects-based monitoring of bioactive compounds associated with municipal wastewater treatment plant effluent discharge to the South Platte River, Colorado, USA. ENVIRONMENTAL POLLUTION. Elsevier Science Ltd, New York, NY, USA, 289: 117928, (2021).

In September, 2015, a water sample was collected downstream of a major metropolitan waste water treatment plant that discharges to the South Platte River, Colorado, USA. The grab sample, 1L, was collected just below the water surface, directly into a pre-cleaned, organic-free, amber glass bottle. The water sample was extracted by solid phase extraction using an Oasis-HLB glass catridge. Cartidges were conditioned sequentially using 5mL each of ethyl acetate, 50:50 methanol (MeOH):dichloromethane (DCM), MeOH, and water. The extract in DMSO was tested in the Attagene cis- and trans-FactorialTM assays (http://www.attagene.com/technology.php; Martin and others 2010; Romanov and others 2008). Data were analyzed using an established analysis pipeline for analyzing ToxCast™ high throughput screening data (Filer and others 2017). "Active hits" in the Attagene assay are included in the data table. This dataset is associated with the following publication: Knapen, D., M. Angrish, M. Fortin, I. Katsiadaki, M. Leonard, L. Mariotta-Casaluci, S. Munn, J. O'Brien, N. Pollesch, L.C. Smith, X. Zhang, and D. Villeneuve. Adverse outcome pathway networks I: Development and applications. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 37(6): 1723-1733, (2018).

In September, 2015, a water sample was collected downstream of a major metropolitan waste water treatment plant that discharges to the South Platte River, Colorado, USA. The grab sample, 1L, was collected just below the water surface, directly into a pre-cleaned, organic-free, amber glass bottle. The water sample was extracted by solid phase extraction using an Oasis-HLB glass catridge. Cartidges were conditioned sequentially using 5mL each of ethyl acetate, 50:50 methanol (MeOH):dichloromethane (DCM), MeOH, and water. The extract in DMSO was tested in the Attagene cis- and trans-FactorialTM assays (http://www.attagene.com/technology.php; Martin and others 2010; Romanov and others 2008). Data were analyzed using an established analysis pipeline for analyzing ToxCast™ high throughput screening data (Filer and others 2017). "Active hits" in the Attagene assay are included in the data table. This dataset is associated with the following publication: Knapen, D., M. Angrish, M. Fortin, I. Katsiadaki, M. Leonard, L. Mariotta-Casaluci, S. Munn, J. O'Brien, N. Pollesch, L.C. Smith, X. Zhang, and D. Villeneuve. Adverse outcome pathway networks I: Development and applications. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 37(6): 1723-1733, (2018).

The present study highlights the utility of bioeffects-based monitoring in conjunction with analytical chemical measurements of surface waters on the Colorado River associated with a historically bioactive wastewater treatment plant effluent. Concurrent with chemical monitoring and in vitro bioactivity measurements, in situ caged fish systems were employed to evaluate the potential bioavailability of predicted biologically-active contaminants associated with ER, GR, and PPAR-associated activities. The present study compares the effects of a wastewater treatment plant facility upgrade on bioactive contaminant loading. This dataset is associated with the following publication: Cavallin, J., W. Battaglin, J. Beihoffer, B. Blackwell, P. Bradley, A. Cole, D. Ekman, R. Hofer, J. Kinsey, K. Keteles, D. Winkelman, and D. Villeneuve. Effects-Based Monitoring of Bioactive Chemicals Discharged to the Colorado River Before and After a Municipal Wastewater Treatment Plant Replacement. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 55(2): 974-984, (2021).

The present study highlights the utility of bioeffects-based monitoring in conjunction with analytical chemical measurements of surface waters on the Colorado River associated with a historically bioactive wastewater treatment plant effluent. Concurrent with chemical monitoring and in vitro bioactivity measurements, in situ caged fish systems were employed to evaluate the potential bioavailability of predicted biologically-active contaminants associated with ER, GR, and PPAR-associated activities. The present study compares the effects of a wastewater treatment plant facility upgrade on bioactive contaminant loading. This dataset is associated with the following publication: Cavallin, J., W. Battaglin, J. Beihoffer, B. Blackwell, P. Bradley, A. Cole, D. Ekman, R. Hofer, J. Kinsey, K. Keteles, D. Winkelman, and D. Villeneuve. Effects-Based Monitoring of Bioactive Chemicals Discharged to the Colorado River Before and After a Municipal Wastewater Treatment Plant Replacement. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 55(2): 974-984, (2021).

Detection Limit Study. This dataset is associated with the following publication: Reddy, T., R. Flick , J. Lazorchak , M. Smith, B. Wiechman, and D. Lattier. Experimental paradigm for in-lab proxy aquatic studies under conditions of static, non flow through chemical exposures. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 34(12): 2796-2802, (2015).