qPCR detection and quantitative results for Northern Pike (Esox lucius) from environmental (e)DNA samples collected along Miller Creek, Kenai Peninsula, Alaska in February and March of 2024

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The dataset contains qualitative detection and quantitative results of Esox Lucius DNA in environmental (e)DNA water samples collected from Miller Creek, Alaska, and analyzed with quantitative PCR.The dataset contains qualitative detection and quantitative results of Esox Lucius DNA in environmental (e)DNA water samples collected from Miller Creek, Alaska, and analyzed with quantitative PCR.

Environmental DNA detection data of Northern pike (Esox lucius) using a portable, field-based platform and a lab-based platform

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We tested the sensitivity of a portable, field-based environmental DNA (eDNA) platform relative to widely used lab-based eDNA approaches for detecting invasive northern pike (Esox lucius) in eight lakes on Alaska’s Kenai Peninsula. Raw data reported in this dataset report detect/non-detect data for technical replicates of water samples.

Environmental DNA detection data of Northern pike (Esox lucius) using a portable, field-based platform and a lab-based platform

공공데이터포털

We tested the sensitivity of a portable, field-based environmental DNA (eDNA) platform relative to widely used lab-based eDNA approaches for detecting invasive northern pike (Esox lucius) in eight lakes on Alaska’s Kenai Peninsula. Raw data reported in this dataset report detect/non-detect data for technical replicates of water samples.

Environmental DNA qPCR detection results and covariates from Idaho, Missouri, Montana, and New York rivers sampled in 2023

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This dataset contains quantitative results from, and covariate attributes of, environmental DNA water samples collected across multiple rivers in 2023. Samples were analyzed for eDNA of round goby in the Hudson River (NY), westslope cutthroat trout (Oncorhynchus clarkii lewisi) in Cherry Creek (MT), lake trout (Salvelinus namaycush) and Spectaclecase mussels (Cumberlandia monodonta) in Big Piney River (MO), and rainbow trout (O. mykiss) and Western Pearlshell (Margaritifera falcata) in Loggers Creek (ID).

Environmental DNA qPCR detection results and covariates from Idaho, Missouri, Montana, and New York rivers sampled in 2023

공공데이터포털

This dataset contains quantitative results from, and covariate attributes of, environmental DNA water samples collected across multiple rivers in 2023. Samples were analyzed for eDNA of round goby in the Hudson River (NY), westslope cutthroat trout (Oncorhynchus clarkii lewisi) in Cherry Creek (MT), lake trout (Salvelinus namaycush) and Spectaclecase mussels (Cumberlandia monodonta) in Big Piney River (MO), and rainbow trout (O. mykiss) and Western Pearlshell (Margaritifera falcata) in Loggers Creek (ID).

Environmental DNA qPCR concentration results and environmental covariates from a Montana headwater stream sampled in 2023

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Environmental DNA quantitative results at the PCR technical replicate level for the target species Arctic Grayling and covariate information associated with these eDNA samples.

Preliminary Assessment of Pacific Lamprey Environmental DNA Samples from Western Washington Rivers.

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qPCR detection results for Pacific Lamprey eDNA surveys from Western Washington Rivers and Streams. Water samples were collected via filtration and shipped to the laboratory for analysis. DNA was extracted from filters using commercially available extraction kits. Lab staff used two existing Pacific Lamprey qPCR eDNA surveys to screen DNA extracts for the presence of Pacific Lamprey DNA. Eight molecular replicates were run for each samples. Results presented in this table show the number of positive detections out of 8 for each sample and the corresponding mean Cq values for each sample with a detection.

qPCR amplification data for tissue and water samples collected from Glacier National Park in 2020 amplified with a Lednia tumana specific assay

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Tabular amplification data for individual technical replicates of tissue and water samples collected from Glacier National Park in 2020 and amplified with a Lednia tumana species-specific qPCR assay. Reported results include fractional detection rates, mean quantification cycles, and estimated gene copies per reaction.

qPCR Results for An Assessment of Naegleria fowleri in Grand Teton National Park, Wyoming

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In 2016, a multidisciplinary team from the U.S. Geological Survey Wyoming-Montana Water Science Center, National Park Service, The Centers for Disease Control and Prevention, and Montana State University’s Department of Microbiology and Immunology in cooperation with the Teton Conservation District, initiated a small study to document the presence or absence of Naegleria fowleri at several hot springs in Grand Teton National Park/John D. Rockefeller, Jr. Memorial Parkway. Over four sampling events in 2016-2017, Naegleria fowleri was detected in several of the Grand Teton National Park/John D. Rockefeller, Jr. Memorial Parkway hot springs for the first time. Additionally, the team found that the prevalence of Naegleria fowleri may be subject to seasonal fluctuations.

Droplet digital PCR data for the detection of Smoky Mountain Madtoms in Great Smoky Mountains National Park

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Environmental DNA water samples were collected at multiple sites in Great Smoky Mountains National Park in 2016 and 2017 and analyzed with a droplet digital PCR assay to screen for the presence of smoky mountain madtoms. Smoky Mountain madtoms were detected within a section of Abrams Creek, known as the only stream in Great Smoky Mountains National Park that supports this species.