This dataset contains quantitative results from, and covariate attributes of, environmental DNA water samples collected across multiple rivers in 2023. Samples were analyzed for eDNA of round goby in the Hudson River (NY), westslope cutthroat trout (Oncorhynchus clarkii lewisi) in Cherry Creek (MT), lake trout (Salvelinus namaycush) and Spectaclecase mussels (Cumberlandia monodonta) in Big Piney River (MO), and rainbow trout (O. mykiss) and Western Pearlshell (Margaritifera falcata) in Loggers Creek (ID).

This dataset contains quantitative results from, and covariate attributes of, environmental DNA water samples collected across multiple rivers in 2023. Samples were analyzed for eDNA of round goby in the Hudson River (NY), westslope cutthroat trout (Oncorhynchus clarkii lewisi) in Cherry Creek (MT), lake trout (Salvelinus namaycush) and Spectaclecase mussels (Cumberlandia monodonta) in Big Piney River (MO), and rainbow trout (O. mykiss) and Western Pearlshell (Margaritifera falcata) in Loggers Creek (ID).

This dataset contains environmental DNA (eDNA) concentrations and spawning adult abundances for sockeye salmon (Oncorhynchus nerka) in Hansen Creek, a small tributary in the Wood River watershed, southeast Alaska, USA. We took temporally- and spatially-replicated eDNA samples during the spawning period (mid-July through August), along with fish counts (live, naturally dead, and killed), dissolved oxygen (DO), discharge, and temperature data at each time of sampling. Samples were collected at each site before adults entered the stream, and then continued to be collected daily or every other day for the next three weeks.

This dataset contains environmental DNA (eDNA) concentrations and spawning adult abundances for sockeye salmon (Oncorhynchus nerka) in Hansen Creek, a small tributary in the Wood River watershed, southeast Alaska, USA. We took temporally- and spatially-replicated eDNA samples during the spawning period (mid-July through August), along with fish counts (live, naturally dead, and killed), dissolved oxygen (DO), discharge, and temperature data at each time of sampling. Samples were collected at each site before adults entered the stream, and then continued to be collected daily or every other day for the next three weeks.

The dataset contains qualitative detection and quantitative results of Esox Lucius DNA in environmental (e)DNA water samples collected from Miller Creek, Alaska, and analyzed with quantitative PCR.The dataset contains qualitative detection and quantitative results of Esox Lucius DNA in environmental (e)DNA water samples collected from Miller Creek, Alaska, and analyzed with quantitative PCR.

The dataset contains qualitative detection and quantitative results of Esox Lucius DNA in environmental (e)DNA water samples collected from Miller Creek, Alaska, and analyzed with quantitative PCR.The dataset contains qualitative detection and quantitative results of Esox Lucius DNA in environmental (e)DNA water samples collected from Miller Creek, Alaska, and analyzed with quantitative PCR.

The data support a study that surveyed the spatial and seasonal variability of northern pike (Esox lucius) environmental DNA (eDNA) between river kilometer 1103 and 1158 in Lake Roosevelt, Washington, USA. The metadata represent qPCR quantification cycle (Cq) values for a northern pike assay performed on water samples collected at the same locations in June and September 2022, water filtration volume, water temperature, and latitude and longitude for each sample site.

Tabular amplification data for individual technical replicates of tissue and water samples collected from Glacier National Park in 2020 and amplified with a Lednia tumana species-specific qPCR assay. Reported results include fractional detection rates, mean quantification cycles, and estimated gene copies per reaction.

Tabular amplification data for individual technical replicates of tissue and water samples collected from Glacier National Park in 2020 and amplified with a Lednia tumana species-specific qPCR assay. Reported results include fractional detection rates, mean quantification cycles, and estimated gene copies per reaction.

A 2007 pilot study was initiated by the University of Alaska – Southeast in which baseline levels of contaminants, including persistent organic pollutants (POPs) and total mercury, were determined in whole bodies of juvenile coho salmon collected in streams in and near national park units in the Southeast Alaska Inventory and Monitoring Network. Although the concentrations of POPs determined in the juvenile coho were relatively low (< 10 ng/g, wet weight), the preliminary findings of this study indicate that, in the Glacier National Park region, salmon from streams with higher density of spawners had increased levels of certain banned chlorinated pesticides (i.e., alpha-chlordane, o,p’-DDT, p,p’-DDD) compared to salmon collected in other streams with lower salmon spawning density. A follow up study will be initiated in spring/summer 2015 to determine concentrations of POPs in resident salmonids, as well as benthic macroinvertebrates collected from five streams that have fish barriers near or adjacent to Glacier National Park. Comparisons in contaminant concentrations and patterns will be made for fish collected below and above the barrier in each stream to help determine if certain classes of POPs are being transported by the spawners. As part of this study, the NWFSC will analyze whole bodies/muscle of salmonids and benthic macroinvertebrate samples POPs using gas chromatography / mass spectrometry (GC/MS). Percent lipid content of samples (with adequate sample mass) will also be determined. These data will be included in a report to the NIH-sponsored IDeA Network of Biomedical Research Excellence. Determining levels of persistent organic pollutants in salmonids and macroinvertebrates.