Gene array data files compared gene expression profiles in liver samples from young (21-45 years) and old (69+ years) men and women to determine changes in the expression of xenobiotic metabolism enzymes and transporters. We identified genes that were differentially expressed in males only, females only, or in all individuals between the young and old using microarray. This dataset is associated with the following publication: Corton, J., J. Lee, J. Liu, H. Ren, B. Vallanat, and M. Devito. Determinants of Gene Expression in the Human Liver: Impact of Aging and Sex on Xenobiotic Metabolism. Experimental Gerontology. Elsevier Science Ltd, New York, NY, USA, 169: 111976, (2022).

The raw values used to create figures 1 and 2 and table 3. This dataset is associated with the following publication: Liu, J., Y. Lu, C. Corton, and C. Klaassen. Expression of cytochrome P450 isozyme transcripts and activities in human livers. XENOBIOTICA. Taylor & Francis, Inc., Philadelphia, PA, USA, 51(3): 279-286, (2021).

Mitochondrial bioenergetics data collected in various viseral organs across different ages in Brown Norway rats. This dataset is associated with the following publication: Pandya, J., M. Valdez, J. Royland, R. MacPhail, P. Sullivan, and P. Kodavanti. Age- and Organ-specific Differences in Mitochondrial Bioenergetics in Brown Norway Rats. Journal of Aging Research. Hindawi Publishing Corporation, New York, NY, USA, 2020(ID7232614): 1-11, (2020).

Accession numbers for microarray datasets used in Oshida et al. Chemical and Hormonal Effects on STAT5b-Dependent Sexual Dimorphism of the Liver Transcriptome. PLoS One. 2016 Mar 9;11(3):e0150284. This dataset is associated with the following publication: Oshida, K., D. Waxman, and C. Corton. Chemical and Hormonal Effects on STAT5b-Dependent Sexual Dimorphism of the Liver Transcriptome.. PLoS ONE. Public Library of Science, San Francisco, CA, USA, 11(3): NA, (2016).

RR-1 is a validation flight to evaluate the hardware operational and science capabilities of the Rodent Research Project on the ISS. RNA DNA and protein were purified from liver tissues from RR-1 mice (female C57BL/6J 16wk old at time of launch). From each group two liver samples were collected and frozen immediately after euthanasia. The rest of the liver samples from each group were collected from frozen carcasses dissected post-flight. RNA-Seq whole genome and RNA BS-Seq (bisulfite sequencing) and proteomic expression profiling were performed. RNA extracted from these tissues was re-sequenced; these data are available as part of GLDS-168 (https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-168).

Data were collected from Xenopus laevis tadpoles exposed to clothianidin and thiamethoxam in two concentrations each, 100 ppm and 20 ppm. Gene expression was generated from brains and livers, and liver cells provided metabolic activity data.

The Rodent Reasearch-1 National Lab (RR-1 CASIS) experiment was performed to study the effect of microgravity on muscle wasting. RNA DNA and protein were purified from RR-1 CASIS (the Center for the Advancement of Science in Space) liver samples. Groups included: Flight (FLT) dissected on-orbit (21 or 22 days after launch); age-matched Ground Controls (GC); and Basal Controls (BC euthanized at time of launch). RNA-Seq whole genome BS-Seq (bisulfite sequencing) and proteomic expression profiling were performed.

These spreadsheets contain the underlying data, calculations, and R code for the figures of the manuscript "Persistent gene expression and DNA methylation alterations linked to carcinogenic effects of dichloroacetic acid". The first tab of every spreadsheet contains descriptions of the data (metadata). For clarifying questions regarding the data, please contact Dr. Brian Chorley (EPA-ORD) at chorley.brian@epa.gov. If you require a review token for the sequencing datasets located in the GEO links (RNAseq and RRBS), please contact Dr. Chorley. This dataset is associated with the following publication: Carswell, G., J. Chamberlin, B. Bennett, P. Bushel, and B. Chorley. Persistent gene expression and DNA methylation alterations linked to carcinogenic effects of dichloroacetic acid. Frontiers in Oncology. Frontiers, Lausanne, SWITZERLAND, 14: 1389634, (2024).

Female C57BL/6CR mice were flown onboard STS-135 for 13 days and returned to Earth for analysis. Livers were collected within 3-4 hours of landing and snap frozen in liquid nitrogen. Liver tissue samples that were used for microarray analysis for GLDS-25 were provided to GeneLab. GeneLab extracted RNA added ERCC control spike-in to the samples and performed RNA-Seq analysis.

The Rodent Research-3 (RR-3) mission was sponsored by the pharmaceutical company Eli Lilly and Co. and the Center for the Advancement of Science in Space to study the effectiveness of a potential countermeasure for the loss of muscle and bone mass that occurs during spaceflight. Twenty BALB/c 18-weeks old female mice (ten controls and ten treated) were flown to the ISS and housed in the Rodent Habitat for 39-42 days. Twenty mice of similar age sex and strain were used for ground controls housed in identical hardware and matching ISS environmental conditions. Basal controls were housed in standard vivarium cages. Spaceflight ground controls and basal groups had blood collected then were euthanized had one hind limb removed and finally whole carcasses were stored at -80 C until dissection. All mice in this data set received only the control/sham injection.